Results 11 to 20 of about 201,027 (156)
本发明涉及一种在蛋白质芯片表面固定蛋白质的方法。该方法使用组分A和组分B的无水乙醇溶液浸泡蛋白质芯片,所述组分A为能够使蛋白质芯片表面呈疏水性的有机化合物;所述组分B为一端含有能够与所需固定的蛋白质分子以共价连接的活性基团、一端含有极性基团的有机化合物;然后使用常规方法活化组分B的极性基团;最后将此蛋白质芯片放入所需固定的蛋白质溶液中浸泡。该方法固定的蛋白质比较稳定;而且能够有效降低蛋白质芯片表面与蛋白质分子间的相互作用,减少蛋白质芯片表面对蛋白质分子的空间构像的影响 ...
王战会, 靳刚
core +2 more sources
本文介绍一种新型光学蛋白质芯片技术 .它通过表面格式化、表面改性和配基固定形成多元生物活性感应表面 ;借助蛋白质的特异结合性和高分辨率的生物光学显微成像技术达到识别和检测蛋白质的目的 .它是一种非标记的多元蛋白质定量分析方法
王战会, 靳刚
core +2 more sources
ABSTRACT Ti‐15Mo‐2.5Ag alloy exhibited a low modulus and strong antibacterial ability but poor bioactivity. To resolve this problem, sandblasting and anodization were used to create a hydrophilic and nano‐Ag2O‐containing TiO2 coating. Subsequently, the microstructure and physicochemical properties were investigated by scanning electron microscopy, X ...
Yanchun Xie +11 more
wiley +1 more source
ABSTRACT Due to their exceptional porosity, structural versatility, and modular coordination chemistry, metal–organic frameworks (MOFs) have emerged as versatile platforms for targeted drug delivery. These structures enable fine‐tuning of pore size, surface activity, and framework stability for the encapsulation of a broad range of therapeutics ...
Rui Hua +9 more
wiley +1 more source
Polyphenol oxidases (PPOs) depletion preserves endogenous proteins at their predicted molecular weights and enhances detectable enzymatic activities. (a) Less RbcL crosslinking in ppo mutant of Nicotiana benthamiana. Leaf extracts were incubated for 1 h, separated on protein gels, stained with Coomassie or analyzed by Western blot with the anti‐RbcL ...
Kaijie Zheng +4 more
wiley +1 more source
ABSTRACT Biodegradable zinc (Zn)‐based materials are considered promising candidates for orthopedic applications because of their favorable mechanical properties, controllable biodegradability, and good biosafety profiles. However, their clinical translation is limited by early‐stage corrosion‐induced cytotoxicity and inflammation, as well as ...
Jinlong Mao +9 more
wiley +1 more source
Read the free Plain Language Summary for this article on the Journal blog. Abstract Soil organic nitrogen (SON) transformation is critical for global nutrient cycling and ecosystem productivity, yet how its responsiveness to climate change differs across diverse land use types remains poorly resolved.
Xinyi Yang +10 more
wiley +1 more source
Network of VAMP714‐dependent interactions during salt stress. Summary Plant responses to salt stress include an altered root architecture mediated by ion imbalances, reactive oxygen species (ROS) accumulation, and altered hormonal responses. Auxin is implicated in these processes.
Jialei Sun +3 more
wiley +1 more source
ABSTRACT Bone restoration is often hindered by the spatiotemporal dysregulation of immune and regenerative signals. Conventional bio‐inert implants, providing only passive support, fail to modulate this microenvironment, leading to infections and poor healing.
Zhizhong Sun +8 more
wiley +1 more source
ABSTRACT Objective To investigate the incidence of pre‐eclampsia (PE) and the predictive performance of the Fetal Medicine Foundation (FMF) first‐trimester PE screening algorithm across pregnancies conceived using assisted reproductive technology (ART), after ovulation induction (OI) and through spontaneous conception (SC).
A. S. L. Kjaer +23 more
wiley +1 more source

