Results 41 to 50 of about 183,892 (152)

Synergetic Ruthenium Clusters and Ligand Motifs in Insoluble Polyoxometalates for Efficient Urea Electrosynthesis

open access: yesRare Metals, Volume 45, Issue 8, August 2026.
ABSTRACT The electrocatalytic co‐reduction of CO2 and nitrate (NO3− ${\text{NO}}_{3}^{-}$) to synthesize urea presents a sustainable alternative to traditional industrial processes, yet its efficiency is fundamentally limited by the kinetically mismatched activation and coupling of CO2 and NO3− ${\text{NO}}_{3}^{-}$.
Jiayu Liu   +9 more
wiley   +1 more source

PCR 技术对Wilson 病基因进行定点突变的研究

open access: yesZhongshan Daxue xuebao. Yixue kexue ban, 2002
【目的】利用聚合酶链反应(PC R)技术对Wilson 病(WD)基因进行体外定点突变的研究。【方法】采用PCR 定点 突变技术, 首先设计两对引物, 将突变位点设计在引物上, 通过重叠延伸法两次PCR 扩增, 扩增片段上含有所需要的突变位 点, 最后将扩增片段克隆入pRc/ CMV 载体中。【结果】DNA 测序表明在预期位点已经发生突变,WD 基因第778 位密码子由 精氨酸(Arg)残基突变为亮氨酸残基(Leu), 用PCR 定点突变技术成功构建Wilso n 病基因突变体。【结论】PCR ...
闫振文   +4 more
doaj  

Synergy Effects of Dual‐Gel‐Assisted Structural Engineering and Bimetallic Doping Regulation Realize Ultrastable High‐Rate Performance of NASICON Cathodes for Sodium‐Ion Batteries

open access: yesRare Metals, Volume 45, Issue 8, August 2026.
ABSTRACT Na‐superionic‐conductor‐type (NASICON) Na4MnV(PO4)3 (NMVP) is an appealing cathode for sodium‐ion batteries (SIBs), but its application is hindered by Jahn–Teller distortions, sluggish Na+ kinetics and poor conductivity, leading to rapid capacity fade.
Huan Liu   +8 more
wiley   +1 more source

21例葡萄糖-6-磷酸脱氢酶缺乏症基因突变型分析

open access: yes四川大学学报. 自然科学版, 2002
G6PD是磷酸戊糖旁路代谢的限速酶 ,也是重要的看家酶 .G6PD缺乏症是人类常见的遗传性疾病 .作者应用突变特异性扩增系统 (ARMS) ,对 2 1例G6PD缺乏症患儿进行基因检测 .结果在2 1例患儿中检出G1388A突变 7例 ,G1376T突变 4例 ,A95G突变 3例 ,未定型 7例 .成都常见的G6PD基因突变型为中国人中常见突变型 ,分析四川人群G6PD基因突变型 ,估计基因突变频率 ,可为四川省G6PD缺乏症的临床防治提供理论依据 .
杨春蕾   +4 more
doaj  

肝硬化患者血浆cfDNA中TERT热点突变率研究

open access: yesZhongguo shiyan zhenduanxue, 2021
目的探讨肝硬化患者血浆游离DNA(cfDNA)中TERT基因-124(C/T)和-146(C/T)热点突变率及其突变与临床指标相关性。方法纳入航天中心医院2019年10月-2020年11月肝硬化患者80例,收集患者临床基本资料。EDTA抗凝全血分离500μL血浆提取cfDNA。TERT热点突变检测采用ARMS-qPCR方法。结果在80例肝硬化患者血浆cfDNA中,检测到TERT热点突变6例(7.5%),其中-124(C/T)4例(5%),-146(C/T)2例(2.5%)。与非突变组比较 ...
薛晓霞, 何美琳, 梁国威
doaj  

Hypertension at 5 months postpartum in women with gestational diabetes

open access: yesUltrasound in Obstetrics &Gynecology, Volume 68, Issue 2, Page 202-210, August 2026.
ABSTRACT Objective Gestational diabetes mellitus (GDM) is associated with future maternal cardiovascular disease, independent of the development of Type‐2 diabetes mellitus. As a major cardiovascular risk factor, we examined the incidence and predictors of hypertension at 5 months postpartum in women with previous GDM.
C. Gómez Fernández   +7 more
wiley   +1 more source

固体破坏的损伤演化诱致突变现象

open access: yes, 1997
介绍了基于细观非线性动力学模型的研究所取得的进展。发现系统显示一种共性特征,称为演化诱致突变,即演化模式从整体稳定向灾变性模式转变,宏观破坏的样本个性行为 ...
白以龙, 柯孚久, 夏蒙棼
core   +2 more sources

Revitalizing Traditional Tank Systems: Case Study Insights From Sri Lanka's Village Tank Cascade Systems for Climate Resilience and Water Governance

open access: yesWorld Water Policy, Volume 12, Issue 3, August 2026.
ABSTRACT Village tank cascade systems (VTCS) are a long‐established form of water management in Sri Lanka's dry zone. However, their role in groundwater recharge, climate adaptation, and water governance remains insufficiently recognized in policy and planning.
Ananthini Nanthakumaran   +1 more
wiley   +1 more source

β-珠蛋白基因突变与红细胞参数的相关研究

open access: yesZhongguo shiyan zhenduanxue, 2012
目的对已明确基因突变的轻型β-珠蛋白生成障碍性贫血患者的红细胞参数进行比较分析,探讨β-珠蛋白基因的突变类型与红细胞参数的相关性。方法分析118例已知基因突变的轻型β-珠蛋白生成障碍性贫血患者的红细胞平均容积、红细胞平均血红蛋白含量、红细胞平均血红蛋白浓度、红细胞体积分布宽度标准差、红细胞体积分布宽度变异系数,对这些参数依据突变类型进行比较。结果在已知β-珠蛋白基因突变中,携带codon41/42(-TTCT)突变的患者红细胞平均容积、红细胞平均血红蛋白含量及红细胞平均血红蛋白浓度高于其它突变患者 ...
彭运生   +4 more
doaj  

Polyphenol oxidase depletion in Nicotiana benthamiana enhances recombinant protein purification and preserves native protein integrity

open access: yesNew Phytologist, Volume 251, Issue 3, Page 1283-1293, August 2026.
Polyphenol oxidases (PPOs) depletion preserves endogenous proteins at their predicted molecular weights and enhances detectable enzymatic activities. (a) Less RbcL crosslinking in ppo mutant of Nicotiana benthamiana. Leaf extracts were incubated for 1 h, separated on protein gels, stained with Coomassie or analyzed by Western blot with the anti‐RbcL ...
Kaijie Zheng   +4 more
wiley   +1 more source

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