Results 101 to 110 of about 2,424 (148)

基于NOD样受体热蛋白结构域相关蛋白3炎症小体信号筛选猴痘病毒调控炎症的关键蛋白

Zhongshan Daxue xuebao. Yixue kexue ban
目的筛选并鉴定猴痘病毒（MPXV）调控NOD样受体热蛋白结构域相关蛋白3（NLRP3）炎症小体信号的关键蛋白。方法以MPXV国内流行毒株的基因组为模板，构建可能具有炎症调控功能的病毒基因表达质粒库（共82个）。将NLRP3、凋亡相关斑点样蛋白（ASC）、半胱氨酸蛋白酶-1（Caspase-1）、含有荧光素酶报告基因的白细胞介素1β前体（pro-IL-1β-Luc）蛋白的真核表达质粒以及病毒基因表达质粒共转染HEK293T细胞，通过检测细胞上清液中的荧光素酶活性来监测NLRP3炎症小体信号通路的活化 ...
罗铭兴, 裴晨辰, 龚李云, 郑新雷, 钱军, 刘全, 郑君   +6 more
doaj  

[Research progress of molecular imprinting technology in the field of disease diagnosis and therapy]. [PDF]

Se Pu
Zhu R, Cai GP, Zheng HJ, Jia Q.
europepmc   +1 more source

[Research advances on the targeted programmed cell death regulatory network for treatment of refractory diabetic wounds]. [PDF]

Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za Zhi
Zhang ZH, He WF.
europepmc   +1 more source

HCV5’－NCR序列克隆和转录重组质粒构建

Zhongshan Daxue xuebao. Yixue kexue ban, 1996
选取ＩＶ基因型ＨＣＶ及中国和台湾代表株ＨＣＶ的５’ＮＣＲ区保守序列合成引物，以ＲＴ－ＰＣＲ从输血后非甲非乙型肝炎病人血浆中扩增一段３００ｂｐ的ｃＤＮＡ片段作目的基因，钝端插入ｐＵＣ１９的ＳｍａＩ切点，构建了ｐＵＨＣＶ－ＮＣ重组质粒。对ｐＵＨＣＶ－ＮＣ分别或混合应用ＨＣＶ序列特异的内外引物和载体序列特异的通用引物进行ＰＣＲ扩增，所用产物分子量均同预期相符；酶切分析查出ＨＣＶ基因所带有和克隆位点融合所产生的两个外源ＮｃｏＩ切点也存在；证实ｐＵＨＣＶ－ＮＣ中克隆基因是ＨＣＶ的５’ＮＣＲ序列且插入方向为反向 ...
doaj  

[Research on Bacteriophage Resistance: Coevolutionary Arms Race Between Bacteria and Phages Drives Novel Antibacterial Therapies]. [PDF]

Sichuan Da Xue Xue Bao Yi Xue Ban
Li R, Wan F, Wei J, Wen Y, Jia P, Lin P, Liu L, Wu M.   +7 more
europepmc   +1 more source

[Bioactive substances delivery for bone repair via nanocomposites: advantages, mechanisms, and emerging trends]. [PDF]

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
Ma Y, Du Z, Wang J, Li X.
europepmc   +1 more source

[Mefloquine HCl promotes DNA repair and alleviates radiation-induced lung epithelial cell injury]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Zhang Y, Wen Q, Huang H, Zhou M, Wang J.
europepmc   +1 more source

[The role and mechanism of chemokine network in promoting osteoarthritis progression by regulating synovial macrophage heterogeneity]. [PDF]

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
Lin Y, Wu L, Chen Q, Shen B.
europepmc   +1 more source

[Heterogeneity in the regulation of cellular stress responses by <i>FUS</i> gene mutations associated with amyotrophic lateral sclerosis]. [PDF]

Zhong Nan Da Xue Xue Bao Yi Xue Ban
Yu C, Zeng W, Meekrathok P, Bu Y, Wang J, Qiu J.   +5 more
europepmc   +1 more source

[Development of a predictive model for severe <i>Mycoplasma</i><i>pneumoniae</i> pneumonia in children younger than 5 years]. [PDF]

Zhongguo Dang Dai Er Ke Za Zhi
Li YT, Long XY, Zhou J, Zhang HY.
europepmc   +1 more source