基于ＤＥＮＶ－ｐｒＭ／Ｅ基因构建ＤＥＮＶ－ＶＬＰ及其免疫学特性
Zhongshan Daxue xuebao. Yixue kexue ban, 2014 摘 要：【目的】 构建和表达登革病毒４型病毒样颗粒，初步研究其免疫学特性。【方法】 用ＲＴ－ＰＣＲ法扩增ＤＥＮＶ４－ｐｒＭ／Ｅ基因，构建重组质粒ｐＧＡＰＺ－ｓｐｒＭ／Ｅ－Ｄ４，转化Ｘ３３酵母菌株，ＳＤＳ－ＰＡＧＥ、ＷｅｓｔｅｒｎＢｌｏｔ鉴定目的蛋白表达。切片电镜检测病毒样颗粒，蔗糖密度梯度离心纯化。免疫三组Ｂａｌｂ／Ｃ小鼠，每组１５只。采用间接ＥＬＩＳＡ检测抗原特异性抗体，间接免疫荧光法检测免疫血清与天然病毒粒子的结合，流式细胞术分析小鼠脾淋巴细胞表型，酶联免疫斑点法（ＥＬＩＳＰＯＴ）检测脾分泌ＩＦＮ ...
doaj
[Nanomaterial-driven Lateral Flow Immunoassay: Technological Development and Application]. [PDF]
Sichuan Da Xue Xue Bao Yi Xue BanZheng J, Hu X, Gu B.
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Zhongshan Daxue xuebao. Yixue kexue ban, 2014 【目的】 探讨ＭＲＩ分子靶向示踪剂细胞间粘附分子链接微米级氧化铁颗粒（ＩＣＡＭ－ＭＰＩＯ）对放射性脑损伤早期无创性诊断的能力及敏感性。【方法】 制备分子靶向示踪剂ＩＣＡＭ－ＭＰＩＯ；ＲＢＩ动物模型建立并分组（靶向组、非靶向组、对照组及钆增强组，每组６只）；通过体外细胞及活体动物实验评价ＩＣＡＭ－ＭＰＩＯ对早期放射性脑损伤的靶向性，以及临床３．０Ｔ磁共振对ＩＣＡＭ－ＭＰＩＯ检测的敏感性。【结果】体外细胞实验显示ＩＣＡＭ－ＭＰＩＯ靶向识别激活内皮细胞，其结合效率约为非靶向组的５倍。活体动物实验显示ＩＣＡＭ ...
doaj
[A preliminary study on the role of platelet mitochondria in the proliferation and metabolism of multiple myeloma cells]. [PDF]
Zhonghua Xue Ye Xue Za ZhiZhang LY, Xiang YH, Li YY, Zhang J.
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[Allergens induce activation of blood CCR3<sup>+</sup> granulocyte subsets in patients with perennial allergic rhinitis]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoWang Q +5 more
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[Advances in the role of miR-378a in skeletal muscle development and diseases]. [PDF]
Zhongguo Dang Dai Er Ke Za ZhiDing ZT, Lan D.
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[Intraperitoneal administration of Allium macrostemon-derived carbon quantum dots alleviates cisplatin-induced acute kidney injury and restores mitochondrial function in mice]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoYang J +7 more
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[Niemann-Pick disease with two missense mutations in SMPD1 gene: a case report and literature review]. [PDF]
Zhonghua Xue Ye Xue Za ZhiWei Y, Guo H, Xu JN, Chen YY, Shi HX.
europepmc +1 more source
[Mechanism of silibinin in renal injury induced by periodontitis in mice]. [PDF]
Hua Xi Kou Qiang Yi Xue Za ZhiHu M +5 more
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