Results 21 to 30 of about 1,793,633 (116)

The Microstructure Evolution and Mechanical Properties of (FeCoCrNi)100‐x(AlCu)x High‐Entropy Alloys

open access: yesRare Metals, Volume 45, Issue 3, March 2026.
ABSTRACT This study systematically investigates the effects of AlCu addition on the microstructural evolution and mechanical properties of (FeCoCrNi)100‐x(AlCu)x (x = 0, 5, 10, 15, 20; at%) high‐entropy alloys (HEAs). The results reveal that the microstructure evolves from coarse equiaxed to refined equiaxed, dendritic, and ultimately dual‐phase ...
Shunfu Xie   +7 more
wiley   +1 more source

piggyBac共转染系统的构建以及转座活性的验证

open access: yes四川大学学报. 自然科学版, 2011
piggyBac(PB)转座子的末端反向重复序列(ITR)和转座酶编码序列(PBase)分别克隆于质粒PB1156、atub-pBac-K10.1TR与包含红色荧光蛋白表达序列(RFP)的质粒pmClherry-N1重组构成供体质粒(Donor);PBase与真核表达载体pEFrF-PGK重组构成辅助质粒(Helper).使用由供体质粒与辅助质粒组成的PB二元共转染系统转染HEK293细胞,结果表明该二元共转染系统具有较好的转座活性,并且在分子水平验证了转座的发生以及使用反向PCR(IPCR ...
缪辉, 刘华华, 吴传芳
doaj  

Adaptive Crystallization of NiFe‐MIL‐55 Nanorods: Atomically Precise Shape Control and Dual‐Function Electrocatalytic Performance

open access: yesRare Metals, Volume 45, Issue 3, March 2026.
ABSTRACT NiFe‐based MIL‐55 metal‐organic frameworks (MOFs) face challenges in achieving atomically precise composition and reliable methods for uniform growth. This study addresses these challenges by applying a slow evaporation crystallization technique to crude colloidal MIL‐55 products from hydrothermal synthesis, resulting in highly uniform ...
H. R. Haris   +10 more
wiley   +1 more source

中国人(广州地区)红细胞GPT遗传性多态现象的特点及血痕GPT的可测性研究

open access: yesZhongshan Daxue xuebao. Yixue kexue ban, 1986
谷丙转氨酶(Glutamic-Pyruvic Transaminase,GPT)是一种具有遗传性多态现象的细胞酶。它在机体各器官组织中广泛存在,尤以肝细胞含量最高。红细胞内GPT含量较少,但也有相当的活性。GPT有两种形式,分别存在于细胞质(可溶性型)和线粒体(线粒体型)之中。GPT分子由两个亚基组成,分子量约114,000道尔顿。
陈跃龙
doaj  

Twin‐Wire In Situ Additive Manufacturing of NiTiNb Eutectic Alloys: Eliminating Unmelted Nb Particles and Tailored Superelasticity

open access: yesRare Metals, Volume 45, Issue 2, February 2026.
ABSTRACT A major challenge in fabricating eutectic NiTiNb alloys is the incomplete melting of Nb due to its much higher melting point than the NiTi matrix. In this work, a novel electron‐beam freeform additive manufacturing strategy is developed to eliminate unmelted Nb particles and achieve a fully eutectic structure through independent dual‐wire ...
Qing‐Da Zhang   +9 more
wiley   +1 more source

人类端粒酶活性重组及其重组特异性

open access: yesZhongshan Daxue xuebao. Yixue kexue ban, 2000
【目的】对Hela 细胞端粒酶进行活性重组并探讨其重组特异性。【方法】以RT-PCR 法从纯化的人端粒酶复合 体中扩增人类端粒酶RNA(hTR)基因片段, 将其克隆至含有SP6 启动子的pGEM3f +质粒中, 构建hTR 的体外转录体系以获 得体外合成的hTR。与端粒酶蛋白组分进行活性重组。四膜虫端粒酶RNA 及16S rRNA 作为重组反应对照组, 以探测其重 组特异性。【结果】经RT-PCR 扩增出450 bp 片段;体外转录重组载体经酶切图谱和PCR 扩增鉴定构建成功;体外转录的 ...
周俊宜, 罗超权
doaj  

基于粒子群算法优化支持向量机的变压器绕组变形分类方法

open access: yesGaoya dianqi, 2020
变压器是电网最为核心的设备,绕组变形是变压器主要的故障类型之一,频率响应分析法(frequency response analysis, FRA)是目前广泛应用的绕组变形检测方法。为提高绕组变形分类诊断的性能,文中提出基于粒子群算法优化支持向量机(particle swarm optimization-support vector machine, PSO-SVM)的变压器绕组变形分类方法,采用数学统计方法提取频率响应曲线的特征参量,并输入到支持向量机模型进行训练,利用粒子群算法优化支持向量机模型参数 ...
刘伟   +4 more
doaj  

In Situ MOF Pyrolysis Construction of Hierarchical Porous Co‐Nanoparticles/Carbon Cloth Composites for Enhanced Electromagnetic Wave Shielding and Absorption

open access: yesRare Metals, Volume 45, Issue 2, February 2026.
ABSTRACT The development of high‐performance electromagnetic protection materials integrating broadband absorption and effective shielding capabilities is hindered by challenges in simultaneously optimizing multiple electromagnetic properties through conventional material designs.
Changlong Du   +9 more
wiley   +1 more source

NK4基因在重组腺病毒中的表达与初步鉴定

open access: yesZhongguo shiyan zhenduanxue, 2003
目的 构建含有肝细胞生长因子 (HGF)NK4基因的重组腺病毒载体 ,为应用HGF/NK4进行肿瘤基因治疗奠定实验基础。方法 应用AdEasy腺病毒载体系统 ,将PCR扩增所得的NK4基因片段与穿梭质粒pShuttle CMV进行连接 ,获得重组质粒pShuttle CMV NK4 ,将该质粒与腺病毒骨架质粒pAdEasy 1共转化大肠杆菌BJ5 183获得重组腺病毒质粒pAdCMV NK4 ,用该质粒转染 2 93细胞包装产生出重组腺病毒rvAdCMV NK4。重组腺病毒分别经电子显微镜技术及RT ...
介建政   +5 more
doaj  

Interfacial Electronic Interactions in Hierarchical Heterostructure Electrocatalyst for Energy‐Saving Hydrogen Production at Industrial Current Densities

open access: yesRare Metals, Volume 45, Issue 2, February 2026.
ABSTRACT Developing low‐energy‐input electrocatalytic systems for efficient hydrogen production is crucial for advancing sustainable energy technologies. The overall hydrazine splitting (OHzS) has emerged as a promising strategy to reduce the overall energy consumption by replacing the sluggish oxygen evolution reaction.
Haozhou Chen   +13 more
wiley   +1 more source

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