[Clinical analysis of donor-purified CD34(+) stem cell boost in 11 patients with poor hematopoietic reconstruction after haploid hematopoietic stem cell transplantation for aplastic anemia]. [PDF]
Zhonghua Xue Ye Xue Za ZhiHe Y +13 more
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, 2003 报道了一种从团头鲂卵壳中提取卵壳蛋白的有效方法。该提取液经SDS PAGE电泳鉴定后 ,证明有三条主要的蛋白带 ,其分子量分别为 6 4KDa,5 6KDa和 5 2KDa ,借助于制备型SDS PAGE电泳和电洗脱分离纯化技术 ...
黎雯,徐盈
core
[Selected donor CD34(+) cell boosts for salvage treatment of poor graft function following allogeneic hematopoietic stem cell transplantation in primary myelofibrosis: 3 cases report]. [PDF]
Zhonghua Xue Ye Xue Za ZhiShi HX +7 more
europepmc +1 more source
[Preparation of purified proteins from fresh Pheretima and their inhibitory effect against pulmonary fibrosis in mice]. [PDF]
Nan Fang Yi Ke Da Xue Xue Bao, 2022 Li S +7 more
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, 2013 本发明涉及一种从菊芋叶片中提取纯化绿原酸的方法。本发明以菊芋叶为原料，经加热回流或超声提取，固液分离后得绿原酸提取液，再经膜分离和膜浓缩后过大孔树脂柱，流加洗杂液洗杂，用洗脱液洗脱至高效液相色谱(HPLC)检测无绿原酸流出，并收集洗脱液，洗脱液减压浓缩，经活性炭脱色，再经喷雾干燥后得绿原酸纯度为94%以上的高纯度绿原酸。该方法不仅样品处理量大，操作简单，适用于工业化生产，而且弥补了金银花等中药资源作为绿原酸提取原料的不足，变废为宝，为提取绿原酸提供一个新的来源，具有较好的经济效益 ...
方圣涛, 夏传海, 孙鹏程
core
[Selenium-containing polyurethane-modified titanium modulates osteogenic differentiation of mesenchymal stem cells and M2 polarization of macrophages]. [PDF]
Beijing Da Xue Xue Bao Yi Xue BanZhai M, Bai X, Fu Y, Xu H, Zhou Y, Lv L.
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[Performance of phenylboronic acid-modified GelMA hydrogel loaded with copper-doped cerium oxide nanozyme and its effect on healing of infected burn wounds in mice]. [PDF]
Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za ZhiShang YJ, Chen YR, Liang Z, Wang XP.
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基因工程乙肝疫苗的研制——Ⅲ.MT-5细胞的HBsAg纯化与鉴定
Zhongshan Daxue xuebao. Yixue kexue ban, 1990 采用超过滤、PEG沉淀和超速离心综合步骤对MT-5细胞培养上清中的HBsAg进行纯化。所得纯化HBsAg经SDS-PAGE银染色结果显示两条多肽,分子量分别为23k和27k,与血源HBsAg的多肽成分相同,为重组HBsAg的两条特异性多肽,且在凝胶中无其它杂蛋白带存在,纯度高。经PAGE银染色结果证实纯HBsAg中的杂蛋白含量符合疫苗生产的要求。经此综合方法提纯的HBsAg回收率可达44.1％以上。
doaj
[Development of high-performance chromatographic materials and their application in herbalome research since 2020]. [PDF]
Se PuFeng J +8 more
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