Results 1 to 10 of about 70,351 (171)
Research on expression of HIV-1 env in baculovirus [PDF]
, 2000 昆虫细胞杆状病毒表达系统(BEVS)是真核表达系统的一种，以一种无需额外辅助的重组昆虫杆状病毒作为表达载体，目前已被应用来表达不同来源的基因，如细菌、病毒、植物和哺乳动物的基因。它具有表达产量高，蛋白经过完善的后处理过程，如内含子剪切、糖基化等特点。其原理是通过外源基因置换病毒的核多角体蛋白基因，使外源基因在核多角体蛋白启动子下高效表达。本论文中采用BEVS表达HIV-1包膜蛋白，目的在于弥补原核表达系统不能表达大片段的、高度糖基化的重组蛋白；并探索其应用在检测试剂中的前景。Baculovirus ...
郭庆
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A link between increased temperature and avian body condition in a logged tropical forest
Conservation Biology, EarlyView.Abstract The combined effects of anthropogenic disturbances, such as logging and climate change, remain poorly understood; yet, they are the main threats to tropical biodiversity. Most tropical African countries lack long‐term climate data, so climate impacts on biodiversity cannot be assessed.
Moreen Uwimbabazi +7 more
wiley +1 more source
Brd4 participates MyoD auto-regulation via its core enhancer element [PDF]
, 2016 肌肉分化决定因子MyoD是调节成肌细胞分化方向的关键转录因子。在成肌细胞形成早期MyoD开始表达出现，一直持续表达到肌肉分化完成，成肌细胞的命运也受到MyoD表达变化的影响。当MyoD最早开始表达时，表达量极低，但其自激活作用会迅速形成正反馈推升其表达，进而激活肌肉功能基因，使成肌细胞分化为肌细胞；而成肌细胞中MyoD表达抑制则会导致肌肉祖细胞生产，由于被MyoD抑制的细胞周期相关基因恢复表达，成肌细胞经过不对称分裂具有再生能力的卫星细胞便会产生，这个步骤对肌肉组织受损应急来说至关重要 ...
邵锐
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Comprehensive framework for assessing and optimizing existing research networks
Conservation Biology, EarlyView.Abstract Conservation, monitoring, and research networks, or collections of ecological research sites unified under a common mission of data collection or a research mission, are essential infrastructure for understanding large landscapes. However, most networks developed opportunistically over decades rather than through systematic design, creating ...
Alyson East +2 more
wiley +1 more source
The effects of axon guidance factor Slit3 on metastasis of human fibrosarcoma [PDF]
, 2015 我们通过在纤维肉瘤细胞HT1080过表达Slit3基因，分析Slit3对肿瘤细胞侵袭转移作用.Transwell侵袭实验中，Slit3过表达后抑制HT1080的迁移侵袭，在裸鼠皮下成瘤实验中，肿瘤体积明显变小、重量明显降低，Slit3过表达后抑制HT1080肿瘤细胞的侵袭转移。此外肿瘤切片HE染色后发现GFP组有病灶点，而slit3过表达则少见，这个结果说明，Slit3显著抑制HT1080细胞转移。slit3过表达后MMP2,MMP9的表达明显降低，而MMP3，MMP13表达量上调 ...
刘咏梅
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Representation of obligate groundwater‐dwelling copepod diversity in European protected areas
Conservation Biology, EarlyView.Abstract Groundwaters sustain diverse surface ecosystems and are populated by metazoan species, mostly invertebrates, that provide fundamental ecological functions and are often of prominent conservation value due to narrow endemism and high phylogenetic rarity.
Francesco Cerasoli +7 more
wiley +1 more source
Involvement of Abnormal MicroRNA Expression in the Mechanism of Drug Resistance with Acute Myeloid Leukemia [PDF]
, 2016 目的：分析miRNA-98的表达异常在急性髓系白血病化疗耐药机制的作用，通过靶向过表达miRNA-98的表达水平探讨以miRNA-98为靶点增加化疗药物敏感性的可能性并分析其可能作用的机制。评价异常表达的血清miRNA-155是否可作为急性髓系白血病的预后标志。 方法：离体实验中，采用qRT-PCR方法检测K562和其耐药株K562/A02中miR-98、E2F1基因表达情况。通过瞬时转染miR-98mimic处理耐药细胞K562/A0248h后，通过qRT-PCR法检测miR-98表达情况；通过qRT-
黄英丹
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Conservation Biology, EarlyView.Abstract Achieving global conservation policy goals requires the ability to set and measure progress toward science‐based targets for biodiversity. The species threat abatement and restoration (STAR) metric was developed to enable actors to set science‐based targets for species.
Louise Mair +17 more
wiley +1 more source
赤点石斑鱼促甲状腺激素TSHβ启动子的克隆及其在斑马鱼胚胎原始性腺和垂体中定位表达 [PDF]
, 2010 促甲状腺激素(Thyroid-stimulating hormone,TSH)具有调节生长,发育,代谢和生殖的功能,但是在低等脊椎动物中的功能研究较少。斜带石斑鱼(Epinephelus coioides)促甲状腺激素TSHβ除在垂体中表达外还在性腺中表达,进一步发现TSHβ也在赤点石斑鱼(Epinephelus akaara)性腺中表达。且在人工诱导的赤点石斑鱼中,随着雄性化的转变,TSHβ转录水平升高。为研究石斑鱼TSHβ特殊表达方式的调控机制,采用基因组步移技术扩增获得赤点石斑鱼TSHβ5 ...
周莉, 李辰昱, 桂建芳, 汪洋
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Taxonomic and functional diversity of urban bees of the world
Conservation Biology, EarlyView.Abstract Bees play a pivotal role in terrestrial environments. Urbanization can affect these organisms and the ecosystem services they provide. However, knowledge of the global diversity of urban bees is limited. Thus, we summarized data on urban bee species identities and occurrences; compared distributions of all bees with those found in urban ...
João C. F. Cardoso +10 more
wiley +1 more source