Results 11 to 20 of about 70,351 (171)
BRCA-1、ERCCmRNA表达水平与在大肠癌铂类化疗预后的相关性研究
Zhongguo shiyan zhenduanxue, 2013 目的探讨以铂类为基础化疗方案的大肠癌患者中乳腺癌敏感蛋白Ⅰ型（BRCA-1）、核苷酸切除修复交叉互补基因（ERCC-1）表达与患者临床病理特征的关系及其预后意义。方法采用实时荧光定量逆转录聚合酶联反应（RT-qPCR）检测54例大肠癌患者组织标本中BRCA-1、ERCCmRNA表达,比较其表达水平与患者临床病理特征和生存时间之间的关系。结果 （1）BRCA-1mRNA低表达20例（37.04%）,高表达34例（62.97%）。BRCA-1mRNA表达水平与淋巴结转移及临床分期有关（P=0.037,P=0.
刘燕文 +4 more
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Functions of DHP and its nuclear receptor on reproductive neuroendocrine in zebrafish (Danio rerio) [PDF]
, 2016 本文研究了DHP对野生型和孕酮核受体敲除纯合型两种类型雄性斑马鱼垂体中促性腺激素β亚基基因（fshb和lhb）、脑中多巴胺合成限速酶酪氨酸羟基化酶基因（th2）及多巴胺二型受体基因（drd2a和drd2b）和促性腺激素释放激素基因（gnrh2和gnrh3）mRNA表达的影响。主要研究结果如下： 1.野生型雄性斑马鱼垂体中促性腺激素β亚基基因（fshb和lhb）mRNA表达呈现周日变化趋势，可分为基础表达期、上升表达期和高峰表达期3个期，高峰表达期的mRNA表达量显著高于其它2个期 ...
王翠丽
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Learner‐Focused Strategy Instruction From the Teachers’ and the Learners’ Perspective
International Journal of Applied Linguistics, EarlyView.ABSTRACT Numerous large‐scale quantitative studies have been conducted to yield a macro‐level picture of the effectiveness of strategy instruction. However, little is understood about learners' actual processing of strategy instruction and interaction with the teachers delivering it.
Isobel Kai‐Hui Wang, Andrew D. Cohen
wiley +1 more source
International Journal of Applied Linguistics, EarlyView.ABSTRACT Translating local research into English as a lingua franca (ELF) connects local scholarship with global readership, but this process remains constrained by language barriers. Large language models (LLMs) offer advanced accessible solutions, but their responsible integration into academic translation requires a deeper understanding of the ...
Yueyue Huang, Yao Yao, Dechao Li
wiley +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2006 【目的】了解常见的皮肤肿瘤组织环氧合酶-2（COX-2）和凋亡相关基因BCL-2表达的情况及其临床意义。初步探讨COX-2与BCL-2在皮肤肿瘤中过表达对肿瘤形成和发展的作用。【方法】免疫组织化学法检测脂溢性角化病（SK）15例，Bowen’s病（BD）15例，基底细胞上皮瘤（BCE）20例，鳞癌（SCC）20例及正常组织5例COX-2和BCL-2蛋白表达。【结果】各标本的肿瘤组织均有COX-2的表达，总体上的阳性率无显著性差异（x^2=0．148，P〉0．05），染色强度有显著性差异（Hc=90．70，
毛越苹 +4 more
doaj
Zhongguo shiyan zhenduanxue, 2022 目的 探讨组织谷氨酸脱氢酶1（GLUD1）与乳腺癌分子分型、临床病理学特征的关系。方法 回顾性分析2014年3月—2017年10月期间在联勤保障部队第九八七医院接受乳腺癌手术的145例患者临床资料，采用实时荧光定量PCR法检测组织GLUD1mRNA表达，采用免疫组化法检测组织GLUD1蛋白表达；比较所有患者肿瘤组织中GLUD1mRNA、蛋白表达与乳腺癌分子分型及临床病理学特征之间的相关性。结果 基于美国公共癌症基因数据库（TCGA）中的数据 ...
刘昊灵 +5 more
doaj
New Phytologist, EarlyView.Network of VAMP714‐dependent interactions during salt stress. Summary Plant responses to salt stress include an altered root architecture mediated by ion imbalances, reactive oxygen species (ROS) accumulation, and altered hormonal responses. Auxin is implicated in these processes.
Jialei Sun +3 more
wiley +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2018 【目的】建立稳定miR-18a过表达及表达的人鼻咽癌细胞系，为研究miR-18a在鼻咽癌发生发展中 的功能奠定基础。【方法】将miR-18a前体的编码基因片段及miR-18a反义寡核苷酸克隆到慢病毒载体中，DNA 测序鉴定；将构建好的载体转染293T细胞，获得重组miR-18a过表达及抑制表达的慢病毒载体，感染人鼻咽癌细 胞CNE1和CNE2，嘌呤霉素筛选获得稳定干扰miR-18a表达及过表达miR-18a的细胞系，PCR及western blot鉴 定。【结果】成功构建真核表达的慢病毒miR ...
常利红 +5 more
doaj
尼罗罗非鱼TCP-1-beta和TCP-1-eta的分子特征及其低温诱导表达 [PDF]
, 2012 为了研究尼罗罗非鱼耐寒性状的分子基础并为耐寒品种选育提供参考,研究从尼罗罗非鱼中克隆了HSP60家族TCP-1-beta和TCP-1-eta基因并对其在低温诱导下的表达特征进行了分析。尼罗罗非鱼TCP-1-beta cDNA长度为1755 bp,包括1605 bp的完整开放阅读框,编码534个氨基酸;尼罗罗非鱼TCP-1-eta cDNA长度1651 bp,包括1638 bp的完整开放阅读框,编码545个氨基酸。与其他物种同源基因的蛋白序列比对结果显示,TCP-1-beta和TCP-1 ...
曾兰 +4 more
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Narrative power in the narrative policy framework
Policy Studies Journal, EarlyView.Abstract The Narrative Policy Framework lacks clear and empirical explanations of power. Yet, the study of narratives is inherently the study of power in shaping policy outputs and decisions. We develop a conceptual model positing that expressions of power (power to, with, and over) may be discovered in narrative constructs (e.g., narrative structure ...
Elizabeth A. Shanahan +8 more
wiley +1 more source