Results 21 to 30 of about 822,650 (184)
, 2007 【目的】构建以基因佐剂hGM-CSF基因为基础的真核表达质粒pIGM-CSF,并在宫颈癌细胞HeLa中表达与鉴定。【方法】采用聚合酶链反应从载体pORF-hGM-CSF中,扩增出hGM-CSF基因,克隆到双顺反子真核表达载体pIRES的多克隆位点B（MCSB）中,构建pIGM-CSF真核表达载体。然后采用脂质体转染法将其转染HeLa细胞,用RT-PCR在RNA水平和ELISA在蛋白水平检测其在真核细胞中的表达。【结果】所克隆hGM-CSF基因片段经测序完全正确;重组质粒转染Hela细胞后,检测有hGM ...
卢燕茹, 江振友
core
The Making of the Right to Food: Tracing Food Policy Conflict and Negotiated Outcomes in Colombia
World Food Policy, Volume 12, Issue 2, November 2026.ABSTRACT The 2016 Peace Agreement between the Colombian Government and the FARC guerrilla group marked a historic step toward ending more than five decades of armed conflict. It also reshaped national food policy with lasting implications. During the negotiations, deep ideological divisions emerged between food sovereignty and food security, and these ...
Felipe Roa‐Clavijo
wiley +1 more source
World Food Policy, Volume 12, Issue 2, November 2026.ABSTRACT Globally, there is collective agreement on the need to advance equity within food systems to promote health equity, food security, and social justice through structural transformation. Food systems are shaped by power asymmetries across scales and actors, which influence the realization of the right to food and constrain the agency of ...
Joan Kaburia, Susan J. Elliott
wiley +1 more source
Partisanship, Deservingness, and the Attitudinal Policy Feedback Process for Social Policy
Policy Studies Journal, Volume 54, Issue 4, November 2026.ABSTRACT In an era of identity‐based partisan polarization, we examine whether social policies can still generate positive attitudinal feedback among beneficiaries. Drawing on nationally representative survey data, we demonstrate that partisanship conditions the policy feedback process through divergent perceptions of group deservingness.
Chris Faricy, Christopher Ellis
wiley +1 more source
Policy Studies Journal, Volume 54, Issue 4, November 2026.ABSTRACT Public consultations are a cornerstone of democratic policymaking, yet the sheer volume and heterogeneity of submissions often overwhelm traditional analysis. Emerging large language models (LLMs) offer new opportunities for making sense of such data, but their performance in contexts with high linguistic and technical diversity remains ...
Edgar Mathevet, Lauriane Cailleux
wiley +1 more source
Rare Metals, Volume 45, Issue 10, October 2026.ABSTRACT To improve the strength–toughness balance of IN718, and alleviate the brittleness commonly associated with refractory high‐entropy alloys, this study proposes a novel strategy to fabricate defect‐free IN718/WMoTaNb heterogeneous multiprincipal element alloy coatings via laser‐directed energy deposition.
Zhao Wang +7 more
wiley +1 more source
PI3KI/Akt1在辐射诱导的乳腺癌细胞自噬发生中表达调控及作用机制
Zhongguo shiyan zhenduanxue, 2010 目的研究不同剂量X线诱导乳腺癌细胞株MCF-7自噬发生过程中Akt表达的变化以及探讨小分子干扰RNA沉默Akt基因后对自噬的影响及可能的作用机制。方法采用实时荧光定量PCR方法检测MCF-7中Akt基因表达,Western blot方法检测Akt蛋白表达;实时荧光定量PCR方法检测Akt过表达和沉默模型中MAP1LC3B基因表达。结果正常照射组MCF-7细胞量效研究表明,在4、8、16 h Akt1表达分别在24、、8Gy区间内呈剂量依赖下降变化,照射后Akt1总体表达均低于假照组 ...
高琳, 刘晓冬, 孔德娟, 马淑梅
doaj
Rare Metals, Volume 45, Issue 10, October 2026.ABSTRACT Wearable sweat sensors face persistent trade‐offs between multiplexed detection, signal stability, and wearing comfort. Here, we present a Janus electronic skin (e‐skin) with dual‐mode functionality, offering both thermal and moisture comfort.
Zhi‐Mei Mu +12 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2007 目的探讨β-连环蛋白（β-cat）在卵巢癌组织中的表达及意义。方法①应用4097点基因芯片对5例卵巢癌及5例正常卵巢组织进行基因表达谱分析,筛查差异表达基因。②对60例卵巢癌组织、24例卵巢瘤组织及24例正常卵巢组织石蜡标本进行免疫组织化学方法染色,检测β-cat的表达情况,分析β-cat的表达与临床分期及组织分级的相关性。结果①基因芯片筛查出卵巢癌差异表达基因58个,其中基因表达增高（上调趋势）30个,基因表达降低（下调趋势）28个,β-cat为表达增高基因。②β ...
张晓霞 +4 more
doaj
, 1999 在转瓶和２Ｌ搅拌反应器中，利用重组杆状病毒ＡｃＮＰＶ感染ｓｆ９昆虫细胞表达尿激酶原。在转瓶中，细胞接毒密度１２×１０６／ｍＬ、ＭＯＩ＝３０时，尿激酶原活性达到１０６５ＩＵ／ｍＬ。研究了尿激酶原表达过程中葡萄糖、乳酸的代谢变化。实验结果表明细胞状态对尿激酶原的表达水平有显著影响。
李佐虎, 常韶华, 孙洪亮
core