Results 31 to 40 of about 13,813 (166)

脂质体流感疫苗免疫效果评价

open access: yesZhongguo shiyan zhenduanxue, 2006
目的对脂质体流感疫苗体液免疫和细胞免疫效果进行评价。方法血凝抑制法(HI)和ELISA法检测特异性抗体;MTT法检测小鼠淋巴细胞增殖情况;流式细胞仪测小鼠T淋巴细胞表面标记;ELISA法检测小鼠肺内溶物中细胞因子含量。结果脂质体流感抗原可激发更强的体液免疫;脾细胞增殖效率,CD4水平和CD4/CD8比例,以及细胞因子(IL-4、IFN-γ)水平均有显著的增强作用。结论脂质体流感疫苗能够刺激机体产生较高的体液免疫,并且能够激发机体产生细胞免疫。
戚凤春   +5 more
doaj  

Preparation and Characterization of Anti-Neuropilin2 Monoclonal Antibody and Its Preliminary Application [PDF]

open access: yes, 2016
研究背景和目的 Neuropilin2(NRP2)是一种多功能的新型受体,在神经系统发育、心血管系统、免疫系统及淋巴管生成等过程中发挥重要作用。最新研究表明,NRP2受体广泛表达于肿瘤细胞表面,与肿瘤血管形成以及肿瘤的发生发展、凋亡、粘附、侵袭和转移等生物学行为密切相关。多种肿瘤细胞均高表达NRP2,提示NRP2可能成为一种新型肿瘤标志物。NRP2通过多种机制调控肿瘤进展,NRP2-b1b2是其发挥作用的重要结构域,NRP2-b1b2结构域可以结合配体Ⅲ型Semaphorin和VEGF ...
杨芸
core  

Analysis of the immunomodulatory properties of mycobacterium cell wall fraction on the cytokine production of peripheral blood mononuclear cells of healthy dogs

open access: yesVeterinary Dermatology, Volume 35, Issue 6, Page 595-604, December 2024.
Background – Mycobacterium cell wall fraction (MCWF) is derived from nonpathogenic Mycobacterium phlei and is used as an immunomodulatory compound in clinical practice, yet its mode‐of‐action requires further research. Objective – To evaluate the host response to MCWF in canine peripheral blood mononuclear cells (PBMCs) by using enzyme‐linked ...
Robert Ward   +9 more
wiley   +1 more source

Development of Novel Immunoassays for Varicella Zoster Virus (VZV) Vaccine and Preliminary Study on Functional Domain of ORF7 protein [PDF]

open access: yes, 2016
水痘-带状疱疹病毒(Varicella-ZosterVirus,VZV)是水痘和带状疱疹的病原体。VZV具有高度传染性,初次感染可引发水痘,并可潜伏于患者背根神经节,在年老或免疫力低下时可能激活导致带状疱疹,具有严重的危害性。接种疫苗是预防VZV感染的有效方式,但目前使用的VZV减毒活疫苗株v-Oka仍具有感染和潜伏人神经系统的能力,安全隐患不容忽视,因此,亟需研制更为安全有效的新型疫苗。高效、精确的免疫分析技术体系是研制新型疫苗的重要基础。VZV是一种基因组约125Kb,拥有70个ORF ...
刘剑
core  

Development of a Pt(II) compound based on indocyanine green@human serum albumin nanoparticles: integrating phototherapy, chemotherapy and immunotherapy to overcome tumor cisplatin resistance

open access: yesRare Metals, Volume 43, Issue 11, Page 6006-6022, November 2024.
Graphical abstract AbstractCisplatin resistance is the main cause for the failure of cancer therapy. To solve the problem, we proposed to develop a novel human serum albumin (HSA) nanoplatform to integrate chemotherapy, photothermal therapy (PTT) and immunotherapy.
Xue‐Yu Man   +7 more
wiley   +1 more source

抗原致敏外周血树突状细胞治疗脑胶质瘤的实验研究

open access: yesZhongguo shiyan zhenduanxue, 2008
目的观察树突状细胞(DCs)从凋亡脑胶质瘤细胞获取抗原后,体外诱导抗肿瘤免疫应答及对胶质瘤细胞的特异性免疫杀伤效果。方法用粒-巨噬细胞集落刺激因子(GM-CSF)加白介素-4(IL-4)从人外周血分化、诱导DCs、γ-射线在体外诱导培养的人脑胶质瘤细胞凋亡,将DCs、T淋巴细胞和凋亡胶质瘤细胞共培养,同时设计不同类型肿瘤细胞(U937及培养胶质瘤细胞)作对照,分离、富集DCs、T淋巴细胞进行免疫应答及肿瘤细胞杀伤试验。结果与凋亡胶质瘤细胞共培养之DCs可以有效提呈胶质瘤细胞抗原,有强烈的免疫应答 ...
苗壮, 付玉, 薄丽娟, 高正
doaj  

Proteomics and Expression of Immunity Related Genes Research in Fujian Oyster Under the Stress of Iron Salt and Vibrio Parahemolyticus [PDF]

open access: yes, 2016
牡蛎隶属于软体动物门(Mollusca)、双壳纲(Bivalvia)珍珠贝目(Pterio)。牡蛎具有很高的食用价值,现已成为中国乃至世界养殖产量最大的经济贝类。然而牡蛎养殖业面临着种种威胁,其中包括了病原体的感染。 铁是脊椎动物血红蛋白的重要组成成分,对机体免疫机制也具有重要作用。但铁对无脊椎动物免疫系统的影响鲜有研究。本论文以福建牡蛎为实验物种,以牡蛎的血淋巴和鳃为实验材料,选择铁盐为胁迫方法和诸多分析技术研究铁盐对牡蛎血淋巴和鳃产生的的影响。 本文采用3mg/L的铁盐胁迫牡蛎96小时 ...
张熠玮
core  

A prospective study evaluating the correlation between local weather conditions, pollen counts and pruritus of dogs with atopic dermatitis

open access: yesVeterinary Dermatology, Volume 35, Issue 5, Page 500-507, October 2024.
Abstract Background Canine atopic dermatitis (cAD) is a hereditary, generally pruritic and predominantly T‐cell‐driven inflammatory skin disease, involving an interplay between skin barrier abnormalities, allergen sensitisation and microbial dysbiosis.
L. Widorn   +2 more
wiley   +1 more source

用低分化鼻咽癌细胞株(CNE2)为靶细胞测定鼻咽癌(NPC)患者ADCC作用

open access: yesZhongshan Daxue xuebao. Yixue kexue ban, 1986
抗体依赖的细胞介导的细胞毒(AntibodyDependent Cell-Mediated Cytotoxicity,ADCC.)是机体细胞免疫与体液免疫两大系统的协调作用,在机体的肿瘤免疫、移植免疫,某些真菌,病毒,寄生虫疾患及某些自身免疫疾患的过程中,均发挥重要作用。ADCC对那些吞噬细胞无法吞噬的体积较大的实体瘤 ...
刘庆伦
doaj  

Effects of Ciji Hua’ai Baosheng Decoction on Hematopoiesis & Tumor Cells Apoptosis Related Factors of Tumor-bearing Chenmotherapy Mice with H22 Hepatoma Carcinoma Cells [PDF]

open access: yes, 2015
目的:研究并证实慈济化癌保生汤(CijiHua’aiBaoshengDecoction,CHBD)对肝癌化疗后机体整体机能的改善作用,明确慈济化癌保生汤对肝癌化疗后机体血细胞的保护作用、造血相关因子的生成作用、促肿瘤细胞凋亡作用,为慈济化癌保生汤在临床的应用提供科学依据。 研究方法:培养昆明(KM)小鼠肝癌H22细胞,稀释为2×107/ml,接种于60只小鼠右前腋下,常规饲养7天后,全部形成皮下移植瘤,以环磷酰胺(CyclophosphamideTabletsandInjections,CTX ...
程尧
core  

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