Results 81 to 90 of about 145,031 (133)

3-methyladenine抑制自噬诱导胰腺癌细胞SW1990失巢凋亡

, 2013
【目的】 探讨3-methyladenine(3-MA)对人胰腺癌SW1990细胞增殖?迁移及失巢凋亡的影响作用及其机制?【方法】 以胰腺癌细胞SW1990及永生化胰腺导管上皮细胞H6C7为研究对象,设3-MA处理组及PBS对照组,采用软琼脂克隆培养法及Poly-HEMA悬浮培养法筛选抵抗失巢凋亡的细胞?Annexin V-FITC/PI双染和TUNEL试剂盒法检测细胞凋亡;免疫荧光法及透射电镜检测细胞自噬;CCK-8法检测细胞增殖; Transwell小室检测细胞迁移能力?【结果 ...

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[Geniposide inhibits retinal cell apoptosis induced by glaucoma through the Hspa1a pathway]. [PDF]

Zhong Nan Da Xue Xue Bao Yi Xue Ban
Hu M, Wang S.
europepmc   +1 more source

[Role and mechanisms of FoxO3a-related signaling pathways in breast cancer cell apoptosis]. [PDF]

Zhong Nan Da Xue Xue Bao Yi Xue Ban
Wang Z, Tang H, Shao X, Zhu Z, Li W, Chen S.   +5 more
europepmc   +1 more source

[Mechanism of Trifolin in Attenuating Hypertension-Induced Renal Cell Apoptosis via Modulation of the MAPK Signaling Pathway]. [PDF]

Sichuan Da Xue Xue Bao Yi Xue Ban
Wu M, Guo Z, Xie Y, Liu H, Chen H, Lin X, Chen R, Shen A, Peng J.   +8 more
europepmc   +1 more source

PKCα由线粒体向细胞核转运与胃癌细胞凋亡诱导密切相关

, 2002
PKC在细胞生长、分化、凋亡和信号转导调节中具有重要作用.通过激光扫描共聚焦显微镜证实:在胃癌BGC-823细胞中,一部分PKCα定位于线粒体,一部分定位在胞浆,细胞经TPA处理后,位于线粒体和胞浆的PKCα向细胞核转运;Western blot检测则发现PKCα蛋白表达水平在TPA处理前后没有发生变化.此外,应用凋亡诱导剂和特异性PKC抑制剂的实验结果进一步证实:胃癌细胞内PKCα由线粒体和胞浆向细胞核转运与细胞凋亡的诱导密切相关.提示PKCα在细胞内的定向转运可能是与细胞凋亡过程相关联的重要事件之一.
丁亮, 叶晓峰, 吴乔, 刘苏, 苏文金   +4 more
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[Niranthin ameliorates Crohn's disease-like colitis in mice via antagonizing intestinal epithelial cell apoptosis and regulating intestinal Th1/Th2 immune homeostasis]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Wen H, Lin J, Zuo L, Liu M.
europepmc   +1 more source

Bcl-xl基因对硝普钠诱导SY5Y细胞凋亡的抑制作用

, 2012
【目的】 研究Bcl-xl基因对成人神经母细胞廇SH-SY5Y细胞毒性的影响.【方法】 将构建好的Bcl-xl基因重组真核表达载体pIRES2-EGFP/Bcl-xl转染SH-SY5Y细胞,并分为正常组、pIRES2-EGFP空质粒转染组、pIRES2-EGFP/Bcl-xl质粒转染组,利用凋亡诱导剂硝普钠(50 μmol/L)诱导各组细胞凋亡,行Hoechst33258细胞染色对比观察各组细胞凋亡情况,通过MTT法分析各组细胞存活率?RT-PCR与Western blotting检测转染后Bcl ...
蒋龙元, 刘淑琼, 陶恩祥, 吴秀勤, 蔡晓冬, 杨炼红   +5 more
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[Elevated expressions of GRP78/CHOP in lupus nephritis: their diagnostic value and association with PERK/IRE1α pathway-mediated renal cell apoptosis]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Wang Y, Zhang W, Fang T, Xie Z, Fan Y, Wang X.   +5 more
europepmc   +1 more source

[Oxidative Stress Induced by High Glucose Aggravates Immunosuppression in Diabetes Mellitus by Promoting Effector T Cell Apoptosis and Regulatory T Cell Differentiation]. [PDF]

Sichuan Da Xue Xue Bao Yi Xue Ban
Ma X, Li Z, Chen W, Zhang W, Zhang D.
europepmc   +1 more source

迷迭香精油诱导宫颈癌Hela细胞凋亡的实验研究

, 2008
【目的】 研究迷迭香精油对体外培养Hela细胞生长的抑制作用65377;【方法】 采用水蒸气蒸馏法提取迷迭香精油,气相色谱-质谱联用仪分析成份65377;以不同浓度的迷迭香精油作用于体外培养的Hela细胞,MTT法测定迷迭香精油抗肿瘤活性,透射电镜观察药物对细胞形态学的影响65377;流式细胞仪检测细胞凋亡率65377; 【结果】 MTT法证明迷迭香精油可显著抑制Hela细胞生长,抑制率与药物浓度正相关,呈现明显的量-效和时-效关系,细胞经药物作用48h后的IC50为0.0736 mg/L ...

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