Results 21 to 30 of about 11,798 (132)
Abstract Background Canine pyoderma is one of the most common presentations in small animal practice, frequently leading to antimicrobial prescribing. Objectives To provide clinicians with antimicrobial treatment guidelines for staphylococcal pyoderma, including those involving meticillin‐resistant staphylococci.
Anette Loeffler +9 more
wiley +1 more source
Reactive oxygen species act as signaling molecules to control root hair initiation and tip growth
Summary Root hairs (RHs) increase the surface area of roots, facilitating nutrient and water uptake and plant anchorage. RHs form from root epidermal cells and elongate by polar tip growth. Reactive oxygen species (ROS) have recently been implicated as important signals that drive RH formation and elongation using both genetic and imaging approaches ...
Megan E. Gerber +2 more
wiley +1 more source
Background – Canine interdigital furunculosis (CIF) is a complex, relapsing inflammatory condition, typically complicated by deep bacterial infections requiring prolonged systemic antibiotics. Hypothesis/Objectives – This split‐body study, where dogs acted as their own control, evaluated whether the adjunctive use of fluorescent light energy (FLE ...
A. Lange +12 more
wiley +1 more source
Background – Canine pyoderma is one of the most common presentations in small animal practice, frequently leading to antimicrobial prescribing. Objectives – To provide clinicians with antimicrobial treatment guidelines for staphylococcal pyoderma, including those involving meticillin‐resistant staphylococci.
Anette Loeffler +9 more
wiley +1 more source
Background – Mycobacterium cell wall fraction (MCWF) is derived from nonpathogenic Mycobacterium phlei and is used as an immunomodulatory compound in clinical practice, yet its mode‐of‐action requires further research. Objective – To evaluate the host response to MCWF in canine peripheral blood mononuclear cells (PBMCs) by using enzyme‐linked ...
Robert Ward +9 more
wiley +1 more source
Background – Lop‐eared rabbits may be predisposed to otitis externa (OE) as a consequence of their ear conformation. Although otoscopy, otic cytological evaluation, and culture are valuable tools in dogs and cats, published data on rabbits remain lacking. Hypothesis/Objectives – This study aimed to assess the utility of otoscopy and cytological results
Nikoleta Makri +8 more
wiley +1 more source
【目的】建立体外成肌细胞分离培养、纯化和鉴定方法。【方法】40 只小鼠分 10 次采用分离消化法培养成肌细 胞,分别用 A、B、C、D 4组不同培养液培养,以 Ficoll梯度液纯化, 进行 Desmin 免疫组化鉴定。【结果】成肌细胞培养后经台盼 蓝检查成活率达 95%以上,在接种后 4~ 5 d增殖达高峰, A、B、C 3 组培养液对成肌细胞的培养效果无差异。Ficoll 分离纯化 的成肌细胞纯度较高,有 97%的成肌细胞 Desmin 胞浆呈阳性反应。【结果】采用 Ficoll纯化以国产血清培养,
王 训 +3 more
doaj
目的探讨地塞米松对多核白细胞诱导的角膜基质细胞胶原降解作用及机制。方法利用角膜基质细胞三维胶原凝胶培养模型,加入多核白细胞或其培养上清及不同浓度地塞米松,在MEM培养液中培养48 h后,通过测定培养上清液中的羟脯氨酸(HYP)的量作为胶原降解的活性单位。免疫印记法分析MMP活性变化。结果在纤维蛋白溶酶原存在的情况下,多核白细胞单独无胶原降解作用,多核白细胞与角膜基质细胞混合培养,及多核白细胞培养上清均能增加角膜基质细胞胶原降解量,差异有显著性 ...
李勤 +5 more
doaj
目的探讨FTY720对体外培养MCF-7细胞增殖及凋亡的影响。方法体外培养MCF-7细胞,将其培养液中加入不同浓度的FTY720,继续培养48小时,MTT法检测MCF-7细胞的增殖情况;瑞士-姬姆萨染色观察细胞形态变化;流式细胞仪检测FTY720对MCF-7细胞的细胞周期、凋亡率的影响。结果当FTY720浓度为6 400 ng/ml时,体外培养MCF-7细胞的增殖受到明显抑制,抑制率为62.0 ...
张淑芳 +3 more
doaj
目的:对表皮细胞在不同的培养方法、不同的培养液及不同底物中的生长情况进行 3 方面的探讨,以便选出较好的一种组合进 行表皮细胞培养,为改善烧伤皮源不足的问题打下基础。方法:①采用人包皮或体皮制成皮块、皮粒、表皮细胞悬液进行液体培养,比较 3 种方 法中表皮细胞生长情况;②应用纯表皮细胞悬液进行空气液体界面培养,平铺平皿培养及培养瓶液体培养,比较表皮细胞贴壁、生长情况;③另 将表皮细胞悬液接种于 3 种不同的培养液,即 RPM I 1640, RPMI 1640+M EM 及 MEM 比较细胞生长差异 ...
朱家源 利天增 苏爱云 郭浩光 唐 庆 朱 斌
doaj

