Results 1 to 10 of about 16,417 (175)

Spatiotemporal changes of construction land expansion in Hangzhou city(杭州城市建设用地扩张的时空演变分析)

open access: yesZhejiang Daxue xuebao. Lixue ban, 2022
基于杭州市1914—2018年间6期近代地图与遥感影像数据,通过目视解译数字化的近代地图,使用面向对象的分类方式对CORONA、Landsat TM和OLI影像进行分类并提取建设用地数据,计算扩张速度、重心变化、景观格局指数和增长类型,分析建设用地的扩张过程与扩张模式。结果表明:(1)近百年来杭州城市建设用地扩张明显,扩张过程主要集中在改革开放后。(2)杭州城市重心经历了“北—西北—北—东南—南”的变化过程,由向单一方向扩张转变为多方向均衡发展的态势。(3)建设用地扩张过程基本符合“扩散—融合”假说 ...
LUHuan(卢奂)   +2 more
doaj   +1 more source

The impact of implicit debt on cities′spatial expansion(隐性债务对城市空间扩张的影响研究)

open access: yesZhejiang Daxue xuebao. Lixue ban, 2022
为推动城市开发与基础设施建设,满足城市新增人口的公共服务需求,地方隐性债务规模快速增长,经济下行加剧了地方政府所面临的债务风险。利用2007—2018年我国285个地级市隐性债务数据,采用固定效应模型,从债务规模与发债主体特征两个视角评估隐性债务对城市空间扩张的驱动机制。主要结论有:(1)隐性债务规模对城市空间扩张有显著正向影响,上期隐性债务规模的增加将推动当期城市建成区扩张。发债规模增加1亿元,城市建成区面积增加0.192 km2,“借债建城”成为地方政府推动城市空间扩张的主要模式。(2 ...
ZHANGJuanfeng(张娟锋)   +3 more
doaj   +1 more source

Detection of Nitrobacteria Populations in Wastewater of Biological Nitrification Stage by Polymerase Chain Reaction Analysis(用PCR技术检测生物硝化池污水中硝化细菌(Nitrobacteria)的研究)

open access: yesZhejiang Daxue xuebao. Lixue ban, 1999
从生物硝化池污水水样中分离了硝化细菌DNA,以其为模板,针对其16SrRNA基因及23SrRNA基因而设计合成了两对引物并分别进行了PCR扩增,扩增产物的2%琼脂糖凝胶电泳结果显示,硝化细菌DNA模板得到了特异性扩增.参照分子量标准(Marker)的电泳结果,被扩增的DNA条带大小分别约为400bp和730bp.实验结果表明PCR 技术可用于污水中硝化细菌的快速检测.
MINGZhen-huan(明镇寰)   +3 more
doaj   +1 more source

Analysis of urban expansion and driving forces in Jiaxing city based on remote sensing image(基于遥感影像的嘉兴市城市扩张与驱动力分析)

open access: yesZhejiang Daxue xuebao. Lixue ban, 2016
在当前高速城市化的背景下,为及时了解嘉兴市的城市发展现状,使用遥感手段提取各时间段内城市的建设区域,并分析了扩张过程的时空特征,再结合社会经济数据探究了城市扩张的驱动力因素.结果表明:嘉兴市在1993〜2013年间城市扩张明显,扩张速度和强度先增后减,扩张方向由西北转向南,扩张模式经历了“主动型-被动型-稳定型”的转变过程,扩张合理性较差,城市扩张速率高于人口增长速率,扩张驱动因子主要为经济发展、产业结构调整和人口增长.
CHENKexin(陈可欣)   +3 more
doaj   +1 more source

Experimental study of ex vivo expanded hematopoietic stem/progenitor in the two step culture from human umbilical cord blood transplanted into NOD/SCID mice(体外分阶段共培养法扩增的脐血造血干/祖细胞移植于NOD/SCID小鼠的实验研究)

open access: yesZhejiang Daxue xuebao. Lixue ban, 2005
研究用分阶段共培养法进行体外扩增的脐血造血干/祖细胞对NOD/SCID小鼠重建造血功能的作用,探讨新扩增技术体系对将来临床移植的可行性.分离脐血单个核细胞,分别用rhSCF+rhG-CSF+rhMDGF组合(非共培养组合)和rhSCF+rhG-CSF+rhMDGF+人骨髓间充质干细胞组合(共培养组合)进行脐血造血干/祖细胞的体外扩增,并对扩增细胞进行流式细胞术和细胞集落形成能力分析.在移植实验中,将经致死剂量射线照射后的NOD/SCID小鼠随机分为3组,每组10只.A组:每只移植8.5 ...
WANGJin-fu(王金福)   +3 more
doaj   +1 more source

利用改良Nest-tetra-primerspecificPCR技术对两种石斑鱼的鉴别 [PDF]

open access: yes, 2009
云纹石斑鱼(EPInEPHEluS MOArA)和褐石斑鱼(E.brunEuS)为石斑鱼属内近缘种,外形相似常被混淆.本研究改良建立了nEST-TETrA-PrIMEr SPECIfIC PCr方法,获得了云纹石斑鱼和褐石斑鱼线粒体dnAnd2基因内的3个特异性条带,分别为内参序列nC1(394bP)、特异性条带nd2-M(268bP)和nd2-b(122bP),以及核基因组中核糖体dnAITS1区的5个特异性条带,分别为内参序列nC2(588bP)、nC3(563bP),特异性条带rdnA-M ...
丁少雄   +4 more
core  

Rapid construction of insect–plant interaction networks via multiplexed long‐fragment DNA metabarcoding and NGS

open access: yesMethods in Ecology and Evolution, EarlyView.
Abstract Molecular identification of insect food webs can accurately reveal complex trophic interactions and serve as a foundation for understanding ecosystem functioning and advancing biodiversity conservation. This process typically involves DNA library construction, high‐throughput sequencing and subsequent steps, including data assembly, annotation,
Xiao‐man Zhang   +9 more
wiley   +1 more source

Effects of parachute science on local research capacity 降落伞科学对当地研究能力的影响

open access: yesConservation Biology, EarlyView.
Abstract Strengthening research capacity is essential to address the global biodiversity crisis. Yet, parachute science often undermines this goal, and its prevalence, costs, and benefits are unclear. We analyzed 13,502 publications on primate research that we extracted from Scopus (1960–2022) to evaluate the effects of parachute science on local ...
Li Yang   +11 more
wiley   +1 more source

New Devices for Droplet Generation and Their Application in Single-Molecule Amplification and Single Cell Manipulation [PDF]

open access: yes, 2016
单分子、单细胞是生物体系的最基本单位。分子之间的不同决定了其引发反应的不同,细胞之间的不同决定了生命活动的不同。因此,深入研究单分子和单细胞对人们探索生命活动本质是十分必要的。与传统研究对象不同,单个分子、单个细胞的研究需要更加精准的研究平台,从而避免大背景的干扰,提高信噪比。微流控芯片,是一种将常规反应小型化、集成化新型的技术。其具有反应迅速、反应体积小、信噪比高、通量高、结果精准、试剂节约等特点,这些优点十分符合目前对单分子、单细胞研究的要求。因此在近些年来,微流控芯片技术在单分子 ...
李星锐
core  

The collective application of shorebird tracking data to conservation

open access: yesConservation Biology, EarlyView.
Abstract Addressing urgent conservation issues, such as the drastic declines of North American migratory birds, requires creative, evidence‐based, efficient, and collaborative approaches. The abundance of over 50% of monitored North American shorebird populations has declined by over 50% since 1980. To address these declines, we developed a partnership
Autumn‐Lynn Harrison   +71 more
wiley   +1 more source

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