Results 11 to 20 of about 270,512 (154)

肝星状细胞通过诱导产生CD8+CD28-T淋巴细胞抑制T细胞增殖

Zhongshan Daxue xuebao. Yixue kexue ban, 2016
【目的】研究肝星状细胞(HSC)是否通过诱导产生CD8+CD28-T淋巴细胞抑制T细胞增殖。【方法】流式细胞仪分选出CD3+ T细胞，用羧基荧光素双乙酸盐琥珀酰亚胺酯(CFSE)标记后与人肝星状细胞系LX2细胞共培养, PHA刺激72 h后,流式细胞仪检测T细胞增殖的百分比;为了研究肝星状细胞调控T细胞增殖的机制，将CD3+ T细胞与LX2细胞共培养3 d，流式细胞仪检测CD8+CD28-T淋巴细胞的百分比。进一步将CD3+ T细胞与LX2细胞共培养3 d，通过流式细胞仪分选出CD8+CD28 ...
doaj   +1 more source

Nrf2通过上调自噬促进C17.2神经干细胞的增殖

Zhongshan Daxue xuebao. Yixue kexue ban, 2016
摘 要: 【目的】 通过使用化合物调控细胞内信号通路,最终引起脑内神经干细胞的增殖和分化?这被为认为是一种崭新的修复受损大脑功能的治疗策略?我们发现Nrf2能够调控C17.2神经干细胞的增殖,并试图阐明其可能的作用机制?【方法】 采用MTT法检测细胞活力,BrdU掺入法检测细胞增殖,western blot法检测细胞内蛋白水平的变化,流式细胞术检测细胞内ROS水平的变化?【结果】 Nrf2的激动剂t-BHQ和SFN可以促进C17.2神经干细胞的增殖,而使用Nrf2的抑制剂brusatol则抑制其增殖 ...
doaj   +1 more source

和厚朴酚对人白血病细胞系U937细胞增殖和凋亡的影响

Zhongshan Daxue xuebao. Yixue kexue ban, 2009
【目的】 探讨和厚朴酚(HNK)对白血病细胞系U937细胞增殖和凋亡的影响65377;【方法】 将U937细胞和正常外周血单核细胞分别与HNK共培养,Hoechst33342荧光染色检测细胞凋亡形态,用噻唑蓝比色法(MTT)检测HNK对细胞增殖的抑制作用;流式细胞仪检测凋亡;罗丹明123检测线粒体膜电位;Caspase3/7活性检测试剂盒检测Caspase3/7活性;荧光定量PCR(FQ-PCR)检测Caspase-365380;Caspase-7 mRNA水平变化65377;【结果 ...
doaj   +1 more source

miR-34a在低氧诱导的大鼠肺动脉平滑肌细胞增殖中的作用 [PDF]

Zhongshan Daxue xuebao. Yixue kexue ban, 2019
【目的】观察miR-34a在低氧诱导的大鼠肺动脉平滑肌细胞增殖中的作用并探讨其可能的机制。【方法】原代分离和培养大鼠肺动脉平滑肌细胞（PASMC），并给予3%低氧处理后，用Real-time PCR法检测miR-34a和Notch1mRNA在大鼠PASMC中的表达；低氧条件下用细胞转染法过表达和抑制miR-34a、沉默Notch1基因的表达后用EDU法观察细胞增殖情况，并用Real-time PCR和Western blot法检测细胞核增殖抗原PCNA的表达。【结果】分离和培养大鼠PASMC并给予3 ...
马兰
doaj   +1 more source

Targeting the PI3K/Akt signaling pathway in pancreatic β‐cells to enhance their survival and function: An emerging therapeutic strategy for type 1 diabetes 一种新兴的1型糖尿病治疗策略:靶向胰腺β细胞中的PI3K/Akt信号通路以增强其存活和功能

Journal of Diabetes, Volume 14, Issue 4, Page 247-260, April 2022., 2022
Highlights Phosphoinositide 3‐kinase (PI3K)/Akt signaling is an emerging promising therapeutic target for diabetes. Modulation of the PI3K/Akt signaling pathway, via protein and peptide‐based biologics, parasite‐derived molecules, plant extracts, or through microRNA interactions, will regulate the processes that determine the function of β‐cells and ...
Inah Camaya, Sheila Donnelly, Bronwyn O'Brien   +2 more
wiley   +1 more source

Relationships between type 2 diabetes, cell dysfunction, and redox signaling: A meta‐analysis of single‐cell gene expression of human pancreatic α‐ and β‐cells 2型糖尿病、细胞功能障碍和氧化还原信号的关系:人胰腺α‐和β‐细胞单细胞基因表达的meta分析

Journal of Diabetes, Volume 14, Issue 1, Page 34-51, January 2022., 2022
Highlights Meta‐analysis of α‐ and β‐cell transcriptomes reveals modified energy metabolism, immune response, autophagy, and cellular stress pathways in cells from type 2 diabetes mellitus. The proportion of immature and senescent β‐cells is increased in islets from type 2 diabetes mellitus.
Emily Sara Marques, Emily Formato, Wenle Liang, Emily Leonard, Alicia R. Timme‐Laragy   +4 more
wiley   +1 more source

Effect of basal insulin supplement therapy on diabetic retinopathy in short‐duration type 2 diabetes: A one‐year randomized parallel‐group trial 在短病程2型糖尿病患者的治疗方案中增加基础胰岛素对糖尿病视网膜病变的影响:一项1年随机平行对照临床研究

Journal of Diabetes, Volume 11, Issue 12, Page 949-957, December 2019., 2019
Highlights This study suggests that basal insulin‐supported oral antidiabetic drugs (OADs) therapy is superior to OADs alone in preventing diabetic retinopathy. This will help clinicians to select a suitable subsequent therapy after initial intensive insulin therapy. Abstract Background In this study, we compared the effect on diabetic retinopathy (DR)
Pan‐Wei Mu, Xi‐Xiang Tang, Ying Tan, Yi‐Na Wang, Shuo Lin, Man‐Man Wang, Jiong Shu, Jing Wang, Yong‐Jun Zhang, Hua Liang, Bai‐Run Lin, Meng‐Yin Cai, Yan‐Ming Chen   +12 more
wiley   +1 more source

Clinical Benefit of Sorafenib Combined with Paclitaxel and Carboplatin to a Patient with Metastatic Chemotherapy‐Refractory Testicular Tumors

The Oncologist, Volume 24, Issue 12, Page e1437-e1442, December 2019., 2019
Abstract Testicular cancer is one of the few tumor types that have not yet benefited from targeted therapy. Still no new active agents for treating this cancer have been identified over the past 15 years. Once patients are refractory to cisplatin‐based chemotherapy, they will be expected to die from testicular cancer.
Bijun Lian, Wenhui Zhang, Tiegong Wang, Qingsong Yang, Zepeng Jia, Huan Chen, Lei Wang, Jing Xu, Wei Wang, Kai Cao, Xu Gao, Yinghao Sun, Chengwei Shao, Zhiyong Liu, Jing Li   +14 more
wiley   +1 more source

3D Printed High‐Strength Hydrogel for Dual Release of Cu2+ and Ag+ Ions to Enhance Bone Repair and Antibacterial Properties

Rare Metals, Volume 45, Issue 6, June 2026.
ABSTRACT Bone restoration is often hindered by the spatiotemporal dysregulation of immune and regenerative signals. Conventional bio‐inert implants, providing only passive support, fail to modulate this microenvironment, leading to infections and poor healing.
Zhizhong Sun, Yifei Wang, Yi Zheng, Minying Li, Longbao Feng, Ziwei Jiang, Hengrui Liu, Rui Guo, Weipeng Sun   +8 more
wiley   +1 more source

Cancellous Bone‐Like Sr‐Doped Hydroxyapatite/Gelatin Porous Scaffolds With Nanofibrous Architecture and Ion Release for Promoting Osteogenesis

Rare Metals, Volume 45, Issue 4, April 2026.
ABSTRACT Constructing a porous scaffold with the essential features of natural cancellous bone, including structural architecture and biochemical cues, is one of the pivotal factors for effective bone regeneration. In this study, a biomimetic composite porous Gelatin fibrous scaffold (SrHA/Gelatin) incorporating ultralong strontium‐doped hydroxyapatite
Yin‐Chuan Wang, Zhao‐Rui Lv, Shuo‐Fu Liang, Meng Zhang, Hong‐Jin Xue, Yi‐Feng Xiong, Yong‐Gang Li, Gui‐Yong Xiao, Yu‐Peng Lu   +8 more
wiley   +1 more source