Results 101 to 110 of about 13,612 (158)

成人骨髓间充质干细胞体外定向诱导分化为神经元样细胞的研究

Zhongguo shiyan zhenduanxue, 2007
目的研究人骨髓间充质干细胞（mesenchymal stem cells,MSCs）向神经细胞分化的可能性。方法利用Per-coll梯度分离及贴壁筛选法分离培养和扩增MSCs;利用bFGF、化学诱导剂DMSO和BHA联合诱导MSCs向神经元转化,观察分化过程中细胞形态的变化,利用免疫细胞化学和RT-PCR方法检测神经元特异性标志物的表达情况。结果经Percoll梯度分离及贴壁筛选法获得了纯度较高的人MSCs,诱导分化后的细胞呈现双极、多极和锥形的典型神经元细胞的形态 ...
辛颖, 李玉林, 张丽红
doaj  

脉冲电刺激对改性纳米羟基磷灰石（op-HA）/PLGA复合材料表面成骨细胞粘附、增殖的影响

Zhongguo shiyan zhenduanxue, 2009
目的研究脉冲电场刺激对改性纳米羟基磷灰石（op-HA）/PLGA复合材料上培养的成骨细胞的粘附和增殖的影响。方法将组织块法原代培养的兔成骨细胞与材料体外培养,在2 V,1Hz的脉冲电刺激下,用FITC荧光染色和NIH Image J软件对图像进行数据分析观察1 d,3 d和7 d成骨细胞在材料表面生长的形态、单个细胞面积比;MTT法检测各组吸光度（A）值。结果随着培养时间的增长,细胞形态由三角形、梭形转变为多角形,单个细胞面积比逐渐增大。op-HA/PLGA表面的单个细胞面积比高于PLGA的 ...
于婷, 章培标, 邬海涛, 崔巍巍, 李波, 陈学思, 刘娅   +6 more
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小鼠骨髓来源的树突状细胞体外诱导分化的实验研究

Zhongguo shiyan zhenduanxue, 2003
目的　研究小鼠骨髓树突状细胞体外诱导分化、成熟及对T细胞增殖的影响 ,为进一步研究树突状细胞的功能及临床肿瘤治疗的应用提供技术方法。方法　应用IL 4、GM CSF培养小鼠骨髓细胞 5～ 7d ,镜下观察细胞形态学变化 ;培养至第 5～ 6d ,加入黑色素瘤 （B16 ）冻融抗原继续培养 1～ 2d ,流式细胞仪检测细胞表面分子CD83、CD86 ,并与同种异体T细胞混合培养 72h ,终止培养前 16h加入 3H TdR（0 .5 μCi 孔 ） ,γ 液体闪烁仪测定cpm值。 结果　IL 4、GM ...
陈玉丙, 张红梅, 刘亚娟, 刘林林, 范洪学   +4 more
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[Chinese expert consensus on the technical and clinical practice specifications of artificial intelligence assisted morphology examination of blood cells (2024)]. [PDF]

Zhonghua Xue Ye Xue Za Zhi
Laboratory Diagnosis Group, Chinese Society of Hematology, Chinese Medical Association.   +2 more
europepmc   +1 more source

[Molecular mechanisms and clinical applications of anti-angiogenic therapy in precision treatment of breast cancer]. [PDF]

Beijing Da Xue Xue Bao Yi Xue Ban
Wang H, Jiang Y.
europepmc   +1 more source

[Niemann-Pick disease with two missense mutations in SMPD1 gene: a case report and literature review]. [PDF]

Zhonghua Xue Ye Xue Za Zhi
Wei Y, Guo H, Xu JN, Chen YY, Shi HX.
europepmc   +1 more source

[Oral homeostasis imbalance under hypoxia]. [PDF]

Hua Xi Kou Qiang Yi Xue Za Zhi
Xu Y, Wang S, Zhou J.
europepmc   +1 more source

[The role and mechanism of chemokine network in promoting osteoarthritis progression by regulating synovial macrophage heterogeneity]. [PDF]

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
Lin Y, Wu L, Chen Q, Shen B.
europepmc   +1 more source

[Advances in oral and craniofacial bone regeneration modulated by stem cells and biomaterials]. [PDF]

Beijing Da Xue Xue Bao Yi Xue Ban
Li Z, Lv L, Zhang X, Xia D, Zhang P, Liu Y, Zhou Y.   +6 more
europepmc   +1 more source

[Silybin inhibits fibrosis after glaucoma filtration surgery in rabbits by promoting fibroblast autophagy]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Zhang Y, Shen Y, Tong X, Duan Y, Luo Y, Guo W.   +5 more
europepmc   +1 more source