Results 41 to 50 of about 1,784 (148)
Zhongshan Daxue xuebao. Yixue kexue ban, 1998 目的: 为明确卵巢早衰者外周血 T 淋巴细胞亚群及补体的特点。方法: 运用免疫酶组化技术———碱性磷酸酶— 抗碱 性磷酸酶( APAAP)法 ,检测卵巢早衰( POF)患者( n =30)、自然绝经后妇女(n=20)及健康育龄妇女( n =30)的外周血 T 淋巴细胞亚群,同时检测其补体水平。结果:①健康育龄妇女、卵巢早衰者及自然绝经者在外周血成熟 T 淋巴细胞总体水平 是一致的。 ②POF 患者及自然绝经后妇女的 CD+ 8 、CD+ 16 T 淋巴细胞数明显升高(P
王文军 邝健全
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Objective: To observe the effects of NaoShu capsule on immune function of aging mice. Methods: Aging mice was induced by subcutaneous injections model was induced by subcutaneous injections with 5% D-galactose at the neck for 6 weeks, IL-2 and IL-6 ...
Chen, Hai-xia +5 more
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Protective Effect and Mechanism of Paeonia suffruticosa Seed Ethanol Extract on Photoaging of HaCaT Cells Induced by UVB [PDF]
Objective: To study the protective effect and mechanism of ethanol extract from Paeonia suffruticosa seed on photoaging of HaCaT cells induced by UVB. Method: The anti-photoaging active ingredients in the ethanol extract of Paeonia suffruticosa seed were
Bin WANG +4 more
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Study of the effect of Strong-bone granules on the differentiation of ROS1728 osteoblasts with silenced ER expression [PDF]
, 2017 目的探讨健骨颗粒对成骨细胞中ERalpha介导的TERT信号通路的调控作用。方法采用雌激素受体拮抗剂ICI182780(Faslodex)阻断成; 骨细胞中雌激素受体alpha(ERalpha)的表达,建立ER抑制的大鼠成骨细胞株ROS1728细胞模型,采用酶联免疫吸附法(ELISA)检测成; 骨细胞液中碱性磷酸酶(alkaline phosphatase,ALP) 、骨钙素(osteocalcin,BGP); 、Ⅰ型胶原(collagen I,Col Ⅰ)的含量。采用实时荧光定量SYBR ...
吴银生 +6 more
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Zhongguo shiyan zhenduanxue目的探讨脐带间充质干细胞源外泌体对子宫内膜基质细胞衰老的影响机制。方法浓度梯度的米非司酮处理子宫内膜基质细胞后选取适宜浓度诱导细胞衰老。培养脐带间充质干细胞并提取外泌体。米非司酮组、米非司酮与外泌体共处理组分别通过Real-time qPCR检测衰老相关分泌表型mRNA的表达量；流式细胞术检测胞内活性氧含量；β-半乳糖苷酶染色检测细胞衰老情况；Western blot检测衰老相关蛋白表达水平。采用SNK-q检验及Dunnett-t检验分别进行多组间比较和组间比较。结果选用40 μmol ...
高心月 +5 more
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人参皂苷Rb1通过Sirt3/SOD2通路延缓高糖诱导的人脐静脉内皮细胞衰老
Zhongshan Daxue xuebao. Yixue kexue ban, 2019 【目的】本研究拟探讨人参皂苷Rb1通过调节沉默信息调节因子3/超氧化物歧化酶2（Sirt3/SOD2）通路延缓高糖诱导的人脐静脉内皮细胞（HUVEC）衰老的作用和机制。【方法】建立高糖（40mmol/L葡萄糖）诱导的HUVEC早熟性衰老模型，根据衰老相关β-半乳糖苷酶（SA-β-Gal）阳性率及Ⅰ型纤溶酶原激活物抑制因子（PAI-1）和P16表达评估HUVEC衰老，并采用AnnexinV-FITC/PI排除细胞凋亡的发生。采用Westernblot检测各组Sirt3与SOD2表达的变化 ...
柯世业 +6 more
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Progress in epigenetic modification of ferroptosis involved in chronic obstructive pulmonary disease [PDF]
Ferroptosis is a special type of programmed cell death, which is different from autophagy, apoptosis and other modes of death, and characterized by mitochondrial shrinkage, membrane density increase and ridge reduction.
CAI Xiaoyu +3 more
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, 1998 现代肿瘤学研究认为肿瘤是多种癌基因突变、扩增或抑癌基因突变或失活累积的结果；按实验肿瘤发生的性质可分为启动、促进和演进3个不同而又相关的过程。不同组织类型肿瘤的癌基因和抑癌基因变化尚无统一的变化规律。端粒酶（TElOMErASE）在绝大多数恶性肿瘤都..
杨善民
core
Protein kinase C and JN K mediate lysophosphatidic acid-induced monocyte chemo-attractant protein-1 expression in human fetal lung fibroblasts [PDF]
, 2017 目的:; 研究蛋白激酶C(PKC)和c-Jun氨基末端激酶(JNK)在溶血磷脂酸(LPA)诱导人肺成纤维细胞(HLF-1)单核细胞趋化蛋白-1(MCP-1; )表达中的作用。方法:培养HLF-1细胞,以不同浓度(0、1、3和10 mumol/L)的LPA处理细胞不同时间(0.5、6、12和24; h)后,ELISA检测细胞培养基上清液中MCP-1的蛋白表达,荧光定量PCR检测MCP-1; mRNA的表达水平。用不同浓度的PKC抑制剂Bisindolylmaleimide I(0、0.1、1和10 ...
傅玉才, 尹齐, 许铭炎, 邓小玲
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Mechanisms of Carboxymethylpachymaran on Regulation of Immune Functions and Plasma Lipid in Aged Mice By iTRAQ-Based Proteomic Technology(基于iTRAQ蛋白质组学技术分析羧甲基茯苓多糖对老龄小鼠免疫功能和血脂调节的机理) [PDF]
Shipin kexue jishu xuebaoNumerous studies have proven that fungal polysaccharides have anti-aging potential. However, the biological mechanisms of anti-aging of fungal polysaccharides have not been fully elucidated.
XU Xiaofei(徐晓飞) +3 more
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