circRNA-miRNA-mRNA调控网络的挖掘以筛选缺血性脑卒中的潜在治疗药物
目的挖掘缺血性脑卒中潜在的circRNA-miRNA-mRNA调控网络及其相应的治疗药物。方法利用GEO数据库发布的数据并选择研究大脑中动脉栓塞(MCAO)小鼠RNA表达水平的3个数据集GSE115697、GSE51586和GSE137482,使用R中的Limma包来筛选差异表达的mRNA(DEmRNA)、DEcircRNA和DEmiRNA。采用MiRWalk、StarBase等多种生物信息学方法结合的综合策略来挖掘与脑卒中相关的circRNA-miRNA-mRNA网络 ...
吴玉敏, 王钟兴, 于瑜
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