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β-环糊精对苯丙氨酸解氨酶反应影响的研究

, 1995
在含有较高浓度底物反式肉桂酸(trans-cinnamic acid,CA)和氨反应介质中,研究了β-环糊精(β-CD)对红酵母苯丙氨酸解氨酶(Phenylalanine ammonia-lyase,PAL,EC4.3.1.5)催化反应的影响。加入适量β-CD可以克服高浓度CA对PAL活性的抑制作用,显著增加产物L-苯丙氨酸(L-phe)的产量。β-CD最适用量随CA浓度升高而增加,其摩尔数量约为CA用量的1/12—1/4。CA浓度范围为100—200mmol/L时,对β-CD有效浓度范围为6 ...
赵健身,杨顺楷, 杨顺楷
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