Results 1 to 10 of about 771,799 (185)
[Advances in separation and analysis of aromatic amino acids in food]. [PDF]
Se Pu, 2022 苯丙酮尿症等代谢病患者因基因缺陷无法正常代谢食物中的芳香族氨基酸(aromatic amino acids, AAA),常规饮食可能会造成永久性生理损伤,低AAA肽是其重要的蛋白质等同物来源。AAA分离分析技术对低AAA肽的制备和检测至关重要。该领域研究者探索了多种高效的吸附、分离材料,从复杂的蛋白质水解液中选择性吸附脱除AAA以制备符合特定氨基酸限量的低AAA肽类食品,或者建立对AAA特异性提取富集的分离分析策略。该文分析了AAA的结构特点与理化性质,总结了近年来基于活性炭 ...
Lu C, Zhang Y, Su Y, Wang W, Feng Y.
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[Advances in applications of activity-based chemical probes in the characterization of amino acid reactivities]. [PDF]
Se Pu, 2023 原创药物的研制得益于蛋白质新靶标的发现,而新靶标的发现依赖于高可信度、高通量的药物-蛋白质相互作用分析方法。蛋白质作为生命功能的执行者,其表达量、空间定位与结构差异直接影响药效的发挥。目前,超过85%的蛋白质尚被认为是无法成药的,主要原因是缺少药物分子靶向的空腔以及相应的反应活性位点。因此,基于蛋白质组学层次实现对氨基酸反应活性位点的表征成为原创共价靶向药物设计的关键,也是克服难以成药靶标蛋白问题的关键。近年来,质谱技术的飞速发展极大地推动了基于蛋白质组学技术的药物-靶蛋白相互作用研究 ...
Li J, Wang G, Ye M, Qin H.
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[Simultaneous determination of glycerol， acetic acid， and sodium bisulfite in compound amino acid injection （14AA） using ion exclusion chromatography]. [PDF]
Se Pu采用离子排斥色谱法，建立了同时测定复方氨基酸注射液（14AA）中甘油、醋酸及亚硫酸氢钠的方法。色谱柱采用Xtimate Sugar‐H柱（300 mm×7.8 mm，5 μm），流动相为7.5 mmol/L 硫酸溶液，等度洗脱，流速为0.5 mL/min，检测波长200 nm，柱温为65 ℃，进样量为10 μL。以紫外检测器进行检测，外标法定量。结果表明，本方法专属性良好，氨基酸成分不干扰甘油、醋酸及亚硫酸根测定，主峰与相邻色谱峰分离度良好，甘油、醋酸及亚硫酸根在一定范围内线性关系良好，相关系数（r）均>
Gu YS, Zuo LM, Gao XL, Shan GZ, Zhao J.
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[Determination of 18 free amino acids in strawberries at different ripening stages by ultra performance liquid chromatography-triple quadrupole mass spectrometry based on hydrophilic interaction]. [PDF]
Se Pu不同成熟时期草莓中游离氨基酸的组成和含量测定对草莓的品质评价和营养研究具有重要意义。本研究建立了基于亲水相互作用的超高效液相色谱-串联质谱技术快速直接测定不同成熟阶段草莓中18种游离氨基酸的分析方法。将经过研磨后的草莓样品用水提取并通过离心和膜过滤后,进行液相色谱-串联质谱分析。采用ACQUITY UPLC Glycan BEH Amide亲水相互作用液相色谱柱(150 mm×2.1 mm, 1.7 μm),以含有5 mmol/L甲酸铵和0.1%甲酸的乙腈水溶液为流动相进行梯度洗脱。采用三重四极杆质谱仪,
Ju M +9 more
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Principal Component Analysis and Comprehensive Evaluation of Free Amino Acids of Different Peppers in Hunan(湖南常见辣椒品种游离氨基酸主成分分析及综合评价) [PDF]
Shipin kexue jishu xuebao, 2021 The free amino acids (FAAs) are involved in the synthesis of flavor compounds in peppers. The types and contents of FAAs are closely related to pepper varieties.
GE Shuai(葛帅) +8 more
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Se pu = Chinese journal of chromatography, 2022 游离氨基酸不仅是一类重要的营养物质,更与中华绒螯蟹独特的滋味和香味密切相关。对游离氨基酸含量进行测定,能为中华绒螯蟹产品的品质评价和风味研究提供有价值的信息。该工作通过优选提取溶剂、优化仪器测定参数,最终使用5%(v/v)高氯酸水溶液提取目标物,采用XDB-C18色谱柱(100 mm×4.6 mm, 1.7 μm),以0.1%(v/v)甲酸水溶液-乙腈为流动相进行梯度洗脱,在电喷雾正离子电离、选择离子扫描模式下进行检测,外标法定量,建立了超高效液相色谱-高分辨质谱(UHPLC-HRMS ...
Ling Gao +8 more
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Se pu = Chinese journal of chromatography, 2021 近年来羊奶粉和骆驼奶粉备受消费者青睐,它们具有潜在的低致敏性,因此成为牛乳不耐受人群尤其是婴幼儿的母乳替代品,其营养价值备受关注。牛奶粉、羊奶粉和骆驼奶粉中氨基酸含量的比较研究鲜有报道。利用酸水解得到游离氨基酸,选择6-氨基喹啉-N-羟基琥珀酰亚胺氨基甲酸酯(AQC)进行柱前衍生,超高效液相色谱分离并检测,外标法定量。18种氨基酸在各自线性范围内线性关系良好,相关系数(r 2)大于0.999;以3倍和10倍信噪比(S/N)确定方法的检出限(LOD)和定量限(LOQ),分别为1.3~2.5 (mg/100
Li Qu +4 more
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Study on Optimization of Extration Process and Nutritional Value of Proteins from Lathyrus sativus Linn.(山黧豆蛋白质的提取及营养价值研究) [PDF]
Shipin kexue jishu xuebao, 2016 Lathyrus sativus Linn. protein was extracted using the alkaline method and amino acid analysis and comprehensive assessment of nutritional value of protein were investigated.
LI Xiaobo(李小波) +6 more
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Journal of Diabetes, Volume 15, Issue 12, Page 1020-1028, December 2023., 2023 Highlights Childhood obesity was significantly associated with the increased risk of insulin resistance (IR). There were 12 metabolites co‐associated with childhood obesity and IR, including seven amino acid and five fatty acid metabolites. The combination of body mass index standard deviation score and key metabolites could effectively predict the ...
Wu Yan +5 more
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Journal of Diabetes, Volume 15, Issue 10, Page 900-910, October 2023., 2023 Highlights Mild acquired impaired thyroid hormone sensitivity was widespread among the general populations. Reduced thyroid hormone sensitivity was related with metabolic syndrome and its components. Impaired thyroid hormone sensitivity might affect the distribution of adipose tissue and muscle.
Fang Lv +6 more
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