Results 41 to 50 of about 2,424 (93)
Zhongshan Daxue xuebao. Yixue kexue ban, 1995 将聚合酶链反应（ＰＣＲ）成份分两组。先加一组含ｄＮＴＰｓ、引物和ＭｇＣｌ２，并加１粒组织切片用石蜡凝固为盖层。再加另一组反应液含待扩增模板、ＴａｑＤＮＡ聚合酶和ＫＣｌ，以与第一组反应液分隔。ＰＣＲ循环时高温可使中隔蜡层融化，两组反应液相混，蜡油上浮作为防蒸发屏障。由此避免室温混合加样和预置导致引物聚体形成和错导非特异扩增，提高ＰＣＲ特异性和灵敏性。作者应用这一技术检测５３例单项抗ＨＢｃ阳性血清，检出ＨＢＶＤＮＡ１３例，检测６９例ＨＢｓＡｇ阳性和抗ＨＢｅ阳性血清，检出ＨＢＶＤＮＡ６１例 ...
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[Research progress of hydrogel-based growth factors for treatment of intervertebral disc degeneration]. [PDF]
Zhongguo Xiu Fu Chong Jian Wai Ke Za ZhiHuo X, Li Z.
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Zhongshan Daxue xuebao. Yixue kexue ban, 1997 摘 要 经温度诱导含正向目的基因工程菌粗提液的DNA 酶活性比宿主菌本身的DNA 酶活性高7 倍以 上, 且比等蛋白含量激发的Raji 细胞粗提液DNA 酶活性高2 倍以上;阳性血清对重组EB 病毒(EBV)-DNA 酶 的中和率高达83 .5 %;用重组酶粗提液和Raji 细胞DNA 酶检测的106 例鼻咽癌(NPC)病人血清抗EBV-DNA 酶抗体(EDAb)水平, 结果无显著性差异, 检出鼻咽癌的效率亦无显著性差异;表明重组酶经进一步提纯并标准 化定量后可用于临床常规检测及大规模普查。
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[Progress in metabolomics of multiple myeloma]. [PDF]
Zhonghua Xue Ye Xue Za ZhiYe X, Sun ZX, Qu XY, Fan L.
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[Effect and mechanism of astaxanthin on the aging of high glucose-treated human skin fibroblasts]. [PDF]
Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za ZhiTang LJ +6 more
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[Extracellular matrix materials and tissue regeneration and repair]. [PDF]
Zhongguo Xiu Fu Chong Jian Wai Ke Za ZhiZhao L, Xie H.
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Zhongshan Daxue xuebao. Yixue kexue ban, 1992 随机引物引导的探针标记足一种比切中移位更为有效的探针标记方法,作者应用这种方法制备32P标记的乙型肝炎病毒（HBV）DNA探针。经液闪测定,探什比放射性为lxl09cpm/ug,（32）P掺入率为71.43％,均比切口移位有关参数上限高出数倍。将这种探针用于尼龙膜载样杂交,在每100cm2的样膜所加探针放射性强度为5 x106cpm、浓度为20ug/L条件下,放射自显影24小时即可出示清晰结果。并且检出HBV DNA灵敏度达0.1Pg,检测HBSAg和HBCAg双阳性血清,HBVDNA阳性率达87 ...
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[Research progress on lactylation modification in pathogenesis of osteoarthritis]. [PDF]
Zhongguo Xiu Fu Chong Jian Wai Ke Za ZhiWu Y +7 more
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[Research progress on cellular metabolic reprogramming in skin fibrosis]. [PDF]
Zhejiang Da Xue Xue Bao Yi Xue BanQian S, Dai S, Guo C, Xu J.
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[Application and advances of exosome-hydrogel system in wound healing]. [PDF]
Zhongguo Xiu Fu Chong Jian Wai Ke Za ZhiQiu Y, Tan Q.
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