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Identification of autoanti-B antibody produced by B blood group, a case report
目的 对1例罕见的B抗原特异性自身抗体标本进行鉴定分析。方法 采用经典试管法、吸收放散法、微柱凝胶法检测血型抗原及抗体。巯基试剂处理血浆、直接抗球蛋白分型鉴定抗体性质。序列特异引物引导的聚合酶链反应进行ABO基因分型,测序分析ABO基因1~7外显子核苷酸序列。结果 ABO血型正反定型不符,正定型B抗原4+,血浆与反定型B细胞凝集2+s,不规则抗体筛查阴性。吸收放散试验证实:存在特异性抗-B,抗体性质为IgM,4℃条件体外溶血试验阳性。SSP基因分型为B/O1;1 ...
Yuan ZHAO, Tongtong LI, Hongyu LIU
doaj +2 more sources
【目的】 建立合适的方法解决混合抗体型自身免疫性溶血性贫血的血型定型和抗体鉴定? 【方法】 分离标本的血清和红细胞,将红细胞进行轻度热放散和氯喹放散实验后,用于ABO正定型?Rh血型抗原分型和后续的自身吸收实验;取适量患者血清,加入等量O型红细胞进行抗体吸收,吸收后的血清用于ABO反定型实验;分别采用PEG法和自身吸收法对自身抗体进行吸收,比较两者的吸收时间?吸收次数?吸收后血清抗体鉴定结果;取适量红细胞进行乙醚放散,放散液用于抗体鉴定?
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【目的】 评价联合检测谷氨酸脱羧酶抗体(GADA)?胰岛细胞抗体(ICA)?胰岛素自身抗体(IAA)等自身抗体对Ⅰ型糖尿病的预报价值?【方法】 采用间接酶联免疫吸附法检测GADA?ICA和IAA;GADA?ICA和IAA不同组合结果的诊断率矩阵决策法? 【结果】Ⅰ型糖尿病患者的GADA?ICA和IAA阳性率分别为68.8%?43.0%和39.8%,显著高于2型糖尿患者和正常组(P < 0.01),其中(GADA阳性率显著高于ICA和IAA(P < 0.05),3种自身抗体联合检测的敏感性,特异性 ...
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Towards fairer conservation: Perspectives and ideas from early‐career researchers
Abstract The Black Lives Matter Movement, which gained unprecedented global momentum in mid‐2020, triggered critical reflection on systemic discrimination of disadvantaged groups across many domains of society. It prompted us, as early‐career researchers (ECRs) in conservation science, to examine our own awareness of ongoing injustices within our field,
Lucy J. Archer +7 more
wiley +1 more source
An empowered sense of “self” is crucial for emotional well‐being and positive relationships. Certain family interaction patterns can disrupt the adult's sense of “mattering” to the child, eroding their perceived self‐efficacy. Mattering can be understood as a felt sense of relational agency which is necessary for experiencing one's interactions as ...
Willem Beckers +2 more
wiley +1 more source
Highlights This prospective study compares 15 patients with immune‐checkpoint induced diabetes mellitus (CPI‐DM) with a propensity score matched cohort of patients with other diabetes types. Diabetic ketoacidosis at onset and autoimmune comorbidity is more frequent in CPI‐DM patients. This can be avoided by regular glucose measurements and education on
Sascha R. Tittel +10 more
wiley +1 more source
The development of fungicide resistance greatly threatens food security and ecological sustainability, and it is urgent need to develop agricultural practices which can ameliorate this problem. Our results show that potato crop with a genetic diversity is associated with a late blight pathogen of higher fungicide sensitivity and lower potential of ...
Li‐Na Yang +8 more
wiley +1 more source
Highlights Several psychiatric disorders were associated with levothyroxine therapy (LT4). Depression, anxiety disorders, and attention‐deficit/hyperactivity disorder showed the strongest relation to LT4. Thyroid autoimmunity itself (no LT4) was not associated with psychiatric disorders.
Alexander Eckert +9 more
wiley +1 more source
一、狼疮肾炎治疗的演变和局限系统性红斑狼疮(systemic lupus erythematosus,SLE)是自身免疫介导的,以免疫性炎症为突出表现的弥漫性结缔组织病。血清抗核抗体为代表的多种自身抗体阳性和多系统受累是SLE的主要临床特征。狼疮肾炎(lupus nephritis,LN)是SLE临床最常见的严重并发症。诊断SLE时50%伴有肾脏损害,而在SLE整体病程中超过60 ...
胡章学
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Highlights A machine learning model can significantly predict imminent development of islet autoimmunity based on environmental, genetic, and metabolic features. The machine learning algorithm feature selection identified type 1 diabetes‐associated single nucleotide polymorphisms from The Environmental Determinants of the Diabetes in the Young (TEDDY ...
Bobbie‐Jo M. Webb‐Robertson +10 more
wiley +1 more source

