[Hesperetin alleviates doxorubicin-induced cardiotoxicity by regulating the AMPK/NLRP3 pathway]. [PDF]
Yan A, Luo M, Chang J, Li X, Zhu J.
europepmc +1 more source
Polarizing macrophages derived from human THP-1 cells in vitro: methods and protocols. [PDF]
Li P, Chen L, Yuan W, Li X, Feng X.
europepmc +1 more source
[Research progress on metal pollutants inducing neurotoxicity through ferroptosis]. [PDF]
Qin Z, Chen Y, Zhao X, Yu S.
europepmc +1 more source
[Lipidomics analysis of glycine-induced bacterial outer membrane vesicles]. [PDF]
Song JY, Qi XL, Guo HZ, Hu LH.
europepmc +1 more source
[Poricoic acid A alleviates dextran sulfate sodium-induced colitis in mice by regulating AMPK/mTOR-mediated autophagy and inhibiting intestinal epithelial cell apoptosis]. [PDF]
Qiao T +7 more
europepmc +1 more source
摘要: 【目的】 探讨人诱导多能干细胞(hiPS细胞)在体外向外周神经元分化的能力。【方法】人诱导多能干细胞在无饲养层培养条件下维持培养,然后在神经培养基中悬浮培养分化为神经球,通过把神经球贴壁在多聚赖氨酸和层粘连蛋白包被的培养板上,利用流式分选细胞方法获得神经嵴干细胞(NCSC),通过把神经嵴干细胞在外周神经诱导培养基中诱导分化,获得外周神经元,利用免疫荧光染色的方法检测神经嵴干细胞和外周神经元的标记物。【结果】贴壁的神经球出现特征性的神经花环(Neural rosettes)结构 ...
doaj
[Analysis of nutritional status before and after different induction therapies in children with Crohn's disease]. [PDF]
Chen LL, Yu Y, Luo YY, Chen J.
europepmc +1 more source
神经干细胞的单层培养及分化过程中HES1和MASH1的表达变化
摘 要: 【目的】 建立神经干细胞(NSC)体外培养方案,使NSC的体外可以得到增殖和分化,并检测NSC分化过程中HES1和MASH1的表达变化。【方法】无菌条件下取SD大鼠胚胎端脑,无血清悬浮培养,形成P2代神经球后,将神经球消化成单细胞,分为神经球悬浮培养和单层贴壁培养,并对两种不同培养方式下的NSC进行干性鉴定和分化能力鉴定。用不同浓度(20、40、80 μg/L)的脑源性神经生长因子(BDNF)诱导NSC分化,选出最合适的浓度。用BDNF最佳诱导浓度诱导NSC分化,Q ...
doaj
[Prognostic value of circulating plasma cell in newly diagnosed multiple myeloma treated with bortezomib, lenalidomide, and dexamethasone]. [PDF]
Liu RR +6 more
europepmc +1 more source
Effects of hirudin for the thrombin-induced expression of adhesive molecules in neutrophils and human umbilical vein endothelial cells [PDF]
Cui, LQ +4 more
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