Results 11 to 20 of about 409,033 (158)
Zhongshan Daxue xuebao. Yixue kexue ban, 2006 【目的】 研究成釉细胞瘤细胞在无血清培养基中的生长特性，建立成釉细胞瘤细胞的无血清培养法。【方法】 用Defined Keratinocyte-SFM（DK-SFM）无血清培养基和DMEM培养成釉细胞瘤细胞，观察细胞的生长特性、细胞形态和传代次数，流式细胞仪（FCM）测定S期细胞比率（SPF）和增殖指数（PI）。【结果】在DK-SFM培养基中，细胞传代6次，平均生长92d；细胞形态清晰，仅见少许散在的成纤维细胞生长：SPF值为12．3％～14．5％，PI值为11．6％～15．3％。在DMEM中 ...
曾东林, 黄洪章, 张彬, 潘朝斌
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Zhongshan Daxue xuebao. Yixue kexue ban, 2010 【目的】 研究不同浓度内毒素(LPS)对NRK52E细胞生长和细胞间缝隙连接功能的影响65377;【方法】 体外培养NRK52E细胞,随机分为对照组及LPS处理组,LPS处理组分别用5个不同浓度的LPS(10 ng/mL65380;50 ng/mL65380;100 ng/mL65380;500 ng/mL65380;1 000 ng/mL)作用24 h65377;采用四甲基偶氮唑盐法检测NRK52E细胞的生长;细胞荧光免疫示踪法测定NRK52E细胞间缝隙连接传递的功能;Western ...
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Zhongshan Daxue xuebao. Yixue kexue ban, 2010 【目的】 研究二烯丙三硫(DATS)对人前列腺癌PC-3细胞的生长抑制作用及相关机制65377; 【方法】 MTT法测定DATS对PC-3细胞生长的影响;细胞形态学,流式细胞仪(FCM)等检测细胞凋亡;western blot65380;比色法分别检测凋亡相关蛋白Bcl-2,Bax和Bcl-xL/Bcl-xS的表达以及Caspase-3活性变化65377; 【结果】 DATS明显抑制PC-3细胞的生长,呈浓度和时间依赖性;DATS作用72 h的IC50为14 μmol/L;14 μmol/L ...
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Zhongshan Daxue xuebao. Yixue kexue ban, 2013 【目的】 探讨硫化氢单独及与顺铂联用对骨肉瘤细胞生长的影响及其可能机制?【方法】 分别用0.2?0.4?0.6?0.8?1.0?2.0?3.0?4.0?5.0 mmol/L NaHS(硫化氢载体)单独或与20 mg/L顺铂共同作用骨肉瘤U2OS细胞24 h,CCK-8比色法法检测细胞存活率;分别用0.4及1.0 mmol/L NaHS作用骨肉瘤U2OS细胞24 h,1.0 mmol/L NaHS单独或与20 mg/L顺铂联用作用骨肉瘤U2OS细胞24 h,细胞单克隆形成法检测各组单克隆数;1.0 ...
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Zhongshan Daxue xuebao. Yixue kexue ban, 2016 摘 要: 【目的】 探讨前列腺素E受体4(EP4)对人甲状腺乳头状癌TPC-1细胞生长的影响?【方法】 体外培养人甲状腺乳头状癌细胞株TPC-1及人甲状腺滤泡上皮细胞株Nthy-ori 3-1,Nthy-ori 3-1细胞作为对照,用ELISA法检测细胞上清液中前列腺素E2(PGE2)含量;用实时荧光定量PCR法检测四种前列腺素E2受体亚型(EP1?EP2?EP3?EP4)mRNA表达水平;用Western Blot法检测EP1-4?PKA及PI3K两种亚型(p110α?p110γ)蛋白的表达情况 ...
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Organ‐specific ozone and nitrogen legacies strengthen substrate control over litter decomposition
Functional Ecology, EarlyView.Read the free Plain Language Summary for this article on the Journal blog. Abstract Elevated tropospheric ozone (O3) and nitrogen (N) deposition are concurrent atmospheric changes that strongly influence terrestrial carbon cycling, yet their combined effects on below‐ground decomposition remain poorly understood.
Xiaofan Hou +8 more
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International Journal of Applied Linguistics, EarlyView.ABSTRACT This study investigates how the learner‐related factors of language dominance, encompassing language history, proficiency, use, and attitude, modulate congruency effects in multi‐word unit (MWU) processing among early bilinguals. Seventy Cantonese–Putonghua bilinguals completed lexical decision tasks measuring reaction time and accuracy for ...
Mingjia Cai, Yuan Liang
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Functional Ecology, Volume 40, Issue 7, Page 2068-2082, July 2026.Read the free Plain Language Summary for this article on the Journal blog. Abstract Grasslands worldwide are experiencing severe degradation due to overgrazing, climate change and anthropogenic disturbances, resulting in substantial declines in biodiversity and ecosystem functioning.
Xiaoqi Wang +5 more
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Integrative Conservation, Volume 5, Issue 2, Page 192-204, June 2026.We developed an effective ex‐situ conservation approach for an endangered orchid species, Dendrobium sinominutiflorum. A compatible fungal strain Tulasnella sp. was obtained by using in‐situ seed baiting technique. The Symbiotic germination with Tulasnella sp. significantly promotes seed germination and seedling growth.
Han‐Xiao Huang +6 more
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Rare Metals, Volume 45, Issue 6, June 2026.ABSTRACT Lung cancer is one of the most common and deadly malignancies worldwide. Although immune checkpoint inhibitors, including PD‐1/PD‐L1 blockers, have benefited a subset of patients, response rates remain limited, and recurrence is frequent because of the immunosuppressive tumor microenvironment (TME), characterized by hypoxia and dysregulated ...
Yi Ma +6 more
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