Results 41 to 50 of about 1,357 (146)
Heterogeneous nuclear ribonucleoprotein H1 and its function in tumor development and metastasis [PDF]
Heterogeneous nuclear ribonucleoprotein (hnRNP) and heterogeneous nuclear RNA (hnRNA) form complexes that regulate RNA splicing and transportation. hnRNPH1 is a member of the hnRNP family of proteins and plays an important role in physiological processes,
FAN Zhisong, FENG Li, ZHANG Zongwen
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Zhongguo shiyan zhenduanxue, 2013 非编码RNAs（non-coding RNAs,ncRNAs）,包括以miRNA、siRNA和piRNA等为代表的短小RNA和长链非编码RNAs（long non-coding RNAs,lncRNAs）。近20年来,ncRNAs的发现和功能研究,大大改变了人们对RNA分子的认识。ncRNAs不仅承担了遗传信息中间载体的辅助性角色,而且在发育和基因表达调控中发挥着复杂而精确的功能[1]。目前,关于ncRNAs在疾病发生、发展和防治中的作用已有大量文献报导,研究主要集中于短ncRNAs在肿瘤 ...
孙牧男, 许淑芬, 方艳秋, 谭岩
doaj
LINC00665通过靶向miR-138/AKT1促进Hela细胞增殖和抑制凋亡的机制研究
Zhongguo shiyan zhenduanxue, 2021 目的探究长链非编码RNA LINC00665通过靶向微小RNA(microRNA,miR)-138/AKT1促进Hela细胞增殖和抑制凋亡的机制。方法分析TCGA数据库中宫颈癌患者miR-138水平与生存之间的关系。通过双荧光素酶报告验证LINC00665靶向miR-138和miR-138靶向AKT1。将宫颈癌细胞系Hela分为4组:对照组、mimic组、mimic+LINC00665组和LINC00665组,通过质粒转染技术过表达miR-138和/或LINC00665。分别通过qPCR和Western
刘晓娟, 谢双双, 张晶, 康燕华
doaj
microRNA-218通过靶向HOXA10抑制肝癌细胞的实验研究
Zhongguo shiyan zhenduanxue, 2019 微小RNA(microRNA,miRNAs)是一类长约22个核苷酸的单链非编码RNA,通过与靶mRNAs完全或不完全互补配对,导致靶基因降解或抑制其翻译,从而在基因的表达调控中发挥着重要作用。研究表明microRNAs参与了肝癌的发生、发展和侵袭转移的全过程[1,2]但是有关miR-218在肝癌(HCC)的生物学功能仍然未知 ...
肖钟迪, 王雅丽, 付凯
doaj
DANCR在人胚胎干细胞向内胚层分化中的调控功能 [PDF]
目的探讨DANCR在人胚胎干细胞（hESC）向内胚层（DE）分化中的作用。方法建立体外诱导hESC向DE分化的体系，检测DANCR的表达水平与DE分化的相关性；利用慢病毒体系敲低hESC的DANCR表达水平，将敲低DANCR的hESC进行DE分化；用qPCR和Western blot方法检测DE分子标志物SOX17和FOXA2，以及原条分子标志物Brachyury （T），EOMES，MIXL1和GSC的表达水平；用Dual luciferase reporter ...
彭丽妹 +3 more
core +1 more source
Cuproptosis-related lncRNA predicts prognosis and immune pathways in osteosarcoma patients [PDF]
Objective To identify cuproptosis-related lncRNAs (CRLs) and use them to construct models to predict survival in osteosarcoma (OS) patients. Methods RNA-seq data of OS patients were downloaded from the TARGET database along with relevant clinical ...
FENG Yanbin +3 more
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Zhongguo shiyan zhenduanxue, 2020 目的研究长链非编码RNA(long non-coding RNA,lncRNA)预测头颈部鳞状细胞癌(head and neck squamous cell carcinoma,HNSC)患者预后的作用。方法下载癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库中HNSC的lncRNA表达数据,使用edgeR包分析HNSC样本中差异表达的lncRNAs。使用COX回归分析筛选预测预后的关键lncRNAs,使用Kaplan ...
向琳, 徐晓晨, 谭君武, 杜波
doaj
Zhongguo shiyan zhenduanxue, 2022 目的 在胆管癌（CCA）中用铁死亡相关长链非编码RNA(lncRNA)建立有较好的预测能力的预后模型。方法 从TCGA数据集下载CCA的转录组RNA-seq数据和相应的临床数据。采用Pearson相关法筛选与铁死亡相关的差异表达的lncRNAs。单因素Cox回归分析用来筛选与预后相关的lncRNAs。通过多因素回归分析构建预后模型，采用受试者工作特征曲线（ROC）下面积和Kaplan-Meier曲线对模型进行评价。结果 筛选出3个铁死亡相关的差异表达lncRNAs:LINC01714 ...
张琳, 石张镇, 白元松
doaj
Clinical prospects and research strategies of long non-coding RNA encoding micropeptides. [PDF]
Zhejiang Da Xue Xue Bao Yi Xue Ban, 2023 Wang X +5 more
europepmc +1 more source
Progress of Microsatellite (GT/CA)n Repeat Polymorphisms in Non-small Cell Lung Cancer [PDF]
Non-small cell lung cancer (NSCLC) is the most important histological type of lung cancer. This disease affects a large number of patients, and the prognosis of advanced patients is poor.
FU Yuting +4 more
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