Construction and identification of interference plasmid targeting on TNFAIP8 [PDF]
, 2015 目的:构建并筛选出干扰效率最佳的TnfAIP8-SHrnA-P SIrEn-rETrO Q干扰质粒。方法:通过生物软件选择3个TnfAIP8基因干扰位点,构建干扰质粒并测序验证,将干扰质粒及对照质粒分别转染至A549细胞,通过rT-PCr、WESTErn blOT检测干扰效率。结果:经rT-PCr和WESTErn-blOT证实TnfAIP8-SHrnA-P SIrEn-rETrO Q干扰质粒能有效干扰并抑制细胞内TnfAIP8基因的表达,通过流式检测发现降低TnVAIP8表达可以提高细胞对A dr5SC
刘忠臣 +7 more
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Disease Burden of Esophageal Cancer Attributable to High BMI and Death Prediction in China and the United States, 1990-2019 [PDF]
Objective To analyze trends in the disease burden of esophageal cancer attributable to high body mass index (BMI) in the Chinese and United States populations from 1990 to 2019 and predict deaths over the next 10 years.
HE Jiahui, HU Qinyong
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Adjuvant strategies for patients with T1b invasion after endoscopic submucosal dissection for esophageal squamous cell carcinoma [PDF]
Objective·To compare the prognostic effects of radical resection of esophageal cancer, concurrent chemoradiotherapy and simple follow-up observation on the prognosis of patients with T1b invasion of superficial esophageal squamous cell carcinoma after ...
LI Chunguang +6 more
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Zhongguo shiyan zhenduanxue, 2012 食管癌的侵袭和转移是导致患者死亡的主要原因,研究表明,基质金属蛋白酶（MMP）-9及其特异性组织金属蛋白酶抑制物（TIMP）-1在肿瘤侵袭转移中发挥重要作用,肿瘤细胞可通过自分化或诱导宿主细胞大量分化MMP和TIMP进入血液,以前的研究多集中在肿瘤组织MMP-9和TIMP-1表达,而本研究旨在探讨食管鳞癌患者血清中MMP-9和TIMP-1水平与肿瘤生物学行为关系。
谢林森
doaj
Preliminary exploration for the mechanism of LLGL2 regulated by SOX2 in esophageal squamous cell carcinoma [PDF]
, 2017 目的 探索SOX2敲低后，关键基因LLGL2在食管鳞癌发生及恶性进程中的作用机制。为食管鳞癌诊断和靶向治疗候选方案的建立奠定基础。 方法 1.应用实时荧光定量PCR和Westernblot分别从mRNA和蛋白水平观察SOX2敲低后，LLGL2的改变情况。 2.采用免疫荧光技术检测食管鳞癌和正常组织临床标本各15例，观察LLGL2的表达情况。 3.利用实时定量PCR技术，检测miR-142-3p在SOX2敲低后的表达情况以验证前期miRNA芯片结果。 4.构建含靶基因LLGL2mRNA3 ...
胡琼
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Advances in molecular mechanisms of cancer origin and early tumorigenesis [PDF]
A comprehensive understanding of the key molecular events underlying the origin and early evolution of malignant tumors is crucial for cancer prevention, early diagnosis, and the development of intervention strategies.
LIN Dongxin, XIAO Xinyi
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高表达的溶血卵磷脂酰基转移酶4促进胰腺癌细胞增殖的作用 [PDF]
目的探讨溶血卵磷脂酰基转移酶4（LPCAT4）在胰腺癌中的表达水平及其对胰腺癌细胞增殖的影响。方法在本研究中，利用在线数据库LinkedOmics在线分析KRAS突变型与野生型胰腺癌患者差异表达基因，使用阿拉巴马大学伯明翰分校癌症数据分析（UALCAN），桑格生信分析平台（Sangerbox 3.0）和表达谱交互分析2（GEPIA2）分析LPCAT4在胰腺癌组织中的表达情况。使用Kaplan-Meier Plotter数据库探究LPCAT4与胰腺癌患者预后的相关性 ...
卢嘉茵 +7 more
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Zhongguo shiyan zhenduanxue, 2014 近年来,生物治疗由于它抗肿瘤的独特特点,在恶性肿瘤的治疗中发挥着重要作用。树突状细胞（Dendritic Cells,DC）作为肿瘤生物治疗中的一种,近年来研究的比较多,尤其在递呈抗原抗肿瘤方面。本实验通过抗原负载的DC对食道癌细胞体外杀伤作用进行研究。1材料和方法1.1材料淋巴细胞分离液（比重1.078）购于中国医学科学院血研所科技公司;GM-CSF,TNF-α,IL-4购于Peprotech公司 ...
赵鑫 +6 more
doaj
Research progress on the role of glutamine metabolism-related proteins in tumor metastasis [PDF]
Tumor metastasis is closely related to high mortality rate of cancer. It is well known that glutamine plays an important role in the malignant progression of cancer.
LIU Xuerou, YANG Yumei, ZHAO Qian, RONG Xiangyu, LIU Wei, ZHENG Ruijie, PANG Jinlong, LI Xian, LI Shanshan
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Research progress of m6A methylation modification in regulating tumor immunity [PDF]
N6-methyladenosine (m6A) is the most prevalent modification that regulates gene expression in eukaryotes. It regulates splicing, degradation, stability, and translation of RNA.
ZHANG Jing, ZHOU Haixia
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