Conservation Biology, EarlyView.Abstract Illegal wildlife trade (IWT) is one of the largest global illegal activities, and it negatively affects biodiversity and sustainable development worldwide. DNA barcoding coupled with high‐throughput sequencing (i.e., metabarcoding) is useful in identifying taxa affected by IWT and has been used routinely for decades.
Maria Joana Ferreira da Silva +9 more
wiley +1 more source
Microeukaryotic community structure and diversity in Xiamen offshore waters and Shenhu Bay, Fujian [PDF]
, 2015 微型真核浮游生物是海洋生态系统的重要组成成分，同时在海洋经典食物链和微食物网中发挥重要作用。该类群个体较小，对环境变化较为敏感，作为生产者、消费者和分解者的微型真核浮游生物在海洋生态系统的生物地球化学过程和生态健康评价等方面有着重要意义。本研究选择厦门岛近海海域和福建省泉州市深沪湾为例，对目标海区进行微型真核浮游生物样品的釆集，运用变性梯度凝胶电泳（DGGE）、克隆测序和高通量测序技术方法对微型真核浮游生物群落组成和多样性进行研究，分析其时空分布和群落结构特征 ...
于凌宇
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Screening and Characteristics of Bacteria and Enzyme for Degradation of Malachite Green from Marine Sediment [PDF]
, 2015 孔雀石绿是一种三苯基甲烷类染料，广泛应用于水产、纺织、造纸等产业，导致水生环境化学需氧量(COD)和生物需氧量(BOD)大幅提高，阻挡光的透射，严重影响水生生物的生长，同时其毒性和致癌性也会通过食物链传递给人类及其他陆生生物。本研究以深海和红树林沉积物中微生物为研究对象，采用富集培养和宏基因组技术对可降解孔雀石绿的微生物及功能基因资源进行挖掘，并对其降解途径及代谢产物进行研究，为近海环境修复提供潜在的、可应用的生物资源 ...
曲武
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Conservation Biology, EarlyView.Abstract Prioritization in conservation is crucial for the development of efficient and effective decision‐making policies. For many decades, the importance of some species and their habitats has been assessed and applied in conservation legislation, but bats and their diurnal roosts have ofbeen overlooked.
David López‐Bosch +6 more
wiley +1 more source
Genetic mapping and quantitative trait loci analysis of growth-related traits in the small abalone Haliotis diversicolor using next-generation sequencing [PDF]
, 2016 杂色鲍(Haliotisdiversicolor)广泛分布于韩国、日本、中国、越南和菲利宾沿岸，是一种具有重要经济价值的养殖鲍种。应用传统的育种方式，杂色鲍育种工作虽然已经取得了一定进展，但是开展分子标记辅助选育仍然是加速该进程的必然选择。下一代测序技术可以高通量、低成本地完成SNP标记的发掘与分型，目前已被广泛应用于遗传研究的各个方面。本文利用下一代测序技术完成了杂色鲍的SNP标记开发与验证、高密度连锁图谱构建、QTL定位和候选基因的关联分析等工作。主要结果如下： (1) 杂色鲍高密度连锁图谱的构建 ...
任鹏
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Comprehensive framework for assessing and optimizing existing research networks
Conservation Biology, EarlyView.Abstract Conservation, monitoring, and research networks, or collections of ecological research sites unified under a common mission of data collection or a research mission, are essential infrastructure for understanding large landscapes. However, most networks developed opportunistically over decades rather than through systematic design, creating ...
Alyson East +2 more
wiley +1 more source
Sequencing and correlation analysis of miRNAome and transcriptome of Larimichthys crocea infected by Cryptocaryon irritans [PDF]
, 2016 大黄鱼（LarimichthyscroceaRichardson，1846）曾是我国的传统四大海洋渔业对象之一，也是我国目前最主要的海水网箱养殖经济鱼类之一，2015年养殖年产量超过14万吨。近年来由于发展模式的制约，养殖管理的缺失，加之养殖环境的严重污染，大黄鱼养殖过程中的各种病毒性疾病，细菌疾病和寄生虫疾病日趋严重，其中以刺激隐核虫（Cryptocaryonirritans）病的危害最为严重。其所感染大黄鱼并导致继发性的细菌感染，造成养殖大黄鱼大面积死亡，对该养殖产业损失极大 ...
乔莹
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Genomic responses to increased temperature and pollinator selection in Brassica rapa L.
New Phytologist, EarlyView.Summary Rapid environmental change reshapes both abiotic stress and biotic interactions, yet it remains unclear how these combined forces structure plants' genomic adaptation. In particular, the joint influence of temperature and pollinator identity, two ecological axes undergoing simultaneous global shifts, has rarely been quantified at genomic ...
Yanqian Ding, Florian P. Schiestl
wiley +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2022 目的本研究通过第二代高通量测序（NGS）检测配对骨肉瘤转移灶和原发灶标本，分析骨肉瘤原发灶与转移灶的基因图谱差异，以期发现促进骨肉瘤发生和转移的相关分子及可能机制。方法12例转移患者的原发灶与转移病灶肿瘤组织样本，使用第二代高通量测序（NGS）进行检测，其中9对panel检测（678个基因）以及3对全外显子检测（WES），分析比较骨肉瘤原发灶与转移灶的基因图谱差异：基因拷贝数变异通过EXCAVATOR检测；DNA层面的融合突变通过Lumpy检测；RNA层面的融合突变通过Defuse + STAR ...
许明贤 +4 more
doaj
Research on Biological Nitrogen Removal by Aerobic Denitrification Process at Low-temperature and the Psychrolerant Mechanism of Functional Bacteria [PDF]
, 2015 本研究针对传统生物脱氮工艺存在能耗大、基建和运行费用高、低温运行效果差等缺陷，采用好氧反硝化工艺处理人工模拟含氮污水，实现好氧条件下同一反应器内全程生物脱氮过程。采用逐步提高曝气时间及曝气强度的方式，以KNO3为氮源，以普通城市污水处理厂的活性污泥为接种泥，实现了好氧反硝化菌的富集及工艺的常温启动。在此基础上，研究了温度对好氧反硝化工艺的性能影响，及低温环境下反应器运行条件的调控效果。此外，针对分离得到的异养硝化-好氧反硝化纯菌的耐冷特性，通过测定其在短期降温过程中ATP含量的变化情况 ...
王语颖
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