Results 51 to 60 of about 10,357 (168)

基于组合神经网络模型的快堆堆芯瞬态热工水力参数预测方法研究

open access: yesHe jishu
对于反应堆热工水力参数的预测,现有的研究多使用单一神经网络的预测方法,但在噪声较大的情况下,单一神经网络不能很好地剔除噪声的影响。本文使用基于经验模态分解法(Empirical Mode Decomposition,EMD)与奇异谱分析法(Singular Spectrum Analysis,SSA)结合自适应径向基神经网络(Radial Basis Function Neural Network,RBF)的组合模型提高堆芯热工参数瞬态预测的精度。采用1/2中国实验快堆(China ...
赵 梓炎   +4 more
doaj   +1 more source

Mating system, brood parasitism and extra-pair behaviour in the Chinese egret (Egretta eulophotes) basing on the microsatellite technology [PDF]

open access: yes, 2015
动物的婚配制度影响着个体的繁殖成功率,直接关系到群体的遗传多样性和种群动态的发展变化,是保护遗传学和生态学领域中非常重要的研究内容。已有学者野外观察到黄嘴白鹭(Egrettaeulophotes)存在婚外交配(EPC)行为,然而,有关黄嘴白鹭的婚配制度、EPC的产生机制以及其是否会导致婚外父权(EPP)现象还未清楚。此外,许多集群繁殖的鸟类都存在种内巢寄生(CBP)现象,而关于黄嘴白鹭巢寄生行为的研究也尚未开展。因此,本研究围绕黄嘴白鹭的婚配制度问题,主要开展以下几方面研究: (1)对3种非损伤性样品 ...
戴宇飞
core  

Abundant top predators increase species interaction network complexity in northeastern Chinese forests

open access: yesJournal of Animal Ecology, Volume 94, Issue 4, Page 745-759, April 2025.
Our study reveals how top predators influence mammalian interaction networks by increasing complexity and redistributing interactions through indirect effects. These findings imply the important role of top predators in structuring communities, offering novel insights into the conservation and management of mammal communities in northeastern China ...
Wen She   +5 more
wiley   +1 more source

Generation and Characterization of HCMV Specific Human Antibody to Explore Antibody Response Profile in HCMV Naturally Infected Individuals [PDF]

open access: yes, 2015
人巨细胞病毒(Humancytomegalovirus,HCMV)是一种常见的疱疹病毒,在人群中普遍感染,儿童感染率约为10%-40%,成人为50%-90%,大部分感染者为无临床症状的健康人。但机体免疫功能低下的人群如器官移植患者及AIDS病人,更易发生HCMV病毒的新发或复发感染,引起致命疾病如肺炎、肝炎、脑炎等,从而导致器官移植失败及术后死亡;同时HCMV病毒也是宫内感染中危害最大的病毒之一,感染率占怀孕妇女的0.5%-2%,尤其是在发展中国家较为常见,孕妇感染后自身多无明显症状或轻微症状 ...
夏琳
core  

Effects of snake fungal disease (ophidiomycosis) on the skin microbiome across two major experimental scales

open access: yesConservation Biology, Volume 39, Issue 2, April 2025.
Abstract Emerging infectious diseases are increasingly recognized as a significant threat to global biodiversity conservation. Elucidating the relationship between pathogens and the host microbiome could lead to novel approaches for mitigating disease impacts.
Alexander S. Romer   +25 more
wiley   +1 more source

高分辨率熔解曲线法检测CBS基因SNP的技术优化

open access: yesZhongguo shiyan zhenduanxue, 2014
目的探索高分辨率熔解曲线法(high resolution melting,HRM)检测胱硫醚β-合酶(CBS)基因SNP位点所需的最佳反应体系和条件,建立快速高效的对CBS基因分型的方法。方法通过普通PCR初步确立引物的退火温度范围,在实时荧光定量PCR(qPCR)-HRM反应中调整确定最佳退火温度。对影响qPCR-HRM的因素包括反应程序、模板DNA、MgCl2再分别进行优化,确立最佳反应体系和反应条件,对在此基础上得出的基因分型结果通过测序验证其准确性 ...
于坤坤   +4 more
doaj  

雌性罗氏鼢鼠肠道微生物群落与功能预测的初步分析

open access: yes野生动物学报, 2021
罗氏鼢鼠(Myospalax rothschildi)是陕西南部农区主要害鼠,研究其与当地环境的相互作用对鼠害防控有重要意义。肠道微生物对宿主行为及生理的调控机制是近年来啮齿类动物防控研究新方向。本研究通过Illumina 高通量测序技术,对雌性罗氏鼢鼠的盲肠样品进行 16S rRNA V3V4区的高通量测序,分析性别及食物因素对罗氏鼢鼠肠道细菌多样性的影响。结果显示,雌性罗氏鼢鼠的肠道内容物中的细菌隶属10门9纲11目13属。肠道革兰氏阳性厚壁菌门(Firmicutes)和革兰氏阴性菌类的变形菌门 ...
陈晓宁 封托 侯祥 安晓雷 王京 常罡
doaj  

Study on the distribution and diversity of nanoflagellate in the Yangtze estuary and continental shelf of East China Sea [PDF]

open access: yes, 2015
本研究应用荧光显微技术、荧光标记颗粒示踪法以及Illumina高通量测序技术,分别于2013年春季、2013年夏季和2013年秋季开展了对长江口及东海陆架区微型鞭毛虫的丰度分布、异养细菌的摄食和群落多样性的研究,并初步探讨了影响微型鞭毛虫分布、摄食和群落多样性的因素。主要结果如下: (1)长江口及东海陆架区微型鞭毛虫的丰度变化范围为0.41-7.15×103cellsml-1,微型鞭毛虫丰度的最高值出现在夏季,最低值出现在秋季,表现出明显的季节变化特征 ...
吴林南
core  

Landscape‐scale predictions of future grassland conversion to cropland or development

open access: yesConservation Biology, Volume 39, Issue 1, February 2025.
Abstract Grassland conservation planning often focuses on high‐risk landscapes, but many grassland conversion models are not designed to optimize conservation planning because they lack multidimensional risk assessments and are misaligned with ecological and conservation delivery scales.
Kevin W. Barnes   +2 more
wiley   +1 more source

小黄鱼jnk1jnk2基因克隆及响应高温应激的表达特征分析

open access: yesHaiyang yu huzhao
JNKs (c-Jun氨基末端激酶)具有调节细胞凋亡的功能,可对多种细胞外信号刺激产生反应。为研究高温对小黄鱼(Larimichthys polyactis)肝脏细胞凋亡的调控机制,对细胞凋亡相关基因jnk进行研究。以NCBI中大黄鱼(Larimichthys crocea) jnk1和jnk2的CDS序列作为参照,克隆获得小黄鱼这两个基因的CDS序列。通过生物信息学分析,发现小黄鱼jnk1 ORF (开放阅读框)序列共1 155 bp,编码384个氨基酸;jnk2 ORF序列共1 263 bp ...
张 天乐   +7 more
doaj   +1 more source

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