Results 11 to 20 of about 4,126 (135)

Structural and functional insights into extreme thermal stability and activity of two GH12 domains of a multidomain glycosidase from a hyperthermophilic euryarchaeon. [PDF]

FEBS J
The multidomain glycosidase MDG from the hyperthermophilic euryarchaeon Thermococcus sp. contains five protein domains: three catalytic domains (GH5, GH12‐1, and GH12‐2) and two cellulose‐binding modules (CBM2). In this study, the GH12‐1 and GH12‐2 domains were individually purified and biochemically characterized.
Zayulina KS, Frolov EN, Stracke C, Klyukina AA, Khusnutdinova AN, Stogios P, Skarina T, Yakunin AF, Golyshin PN, Siebers B, Shugaeva TE, Kublanov IV.   +11 more
europepmc   +2 more sources

Elucidation of the noncovalent interactions driving enzyme activity guides branching enzyme engineering for α-glucan modification. [PDF]

Nat Commun
Branching enzymes (BEs) confer to α-glucans, the primary energy-storage reservoir in nature, a variety of features, like slow digestion. The full catalytic cycle of BEs can be divided in six steps, namely two covalent catalytic steps involving ...
Zong Z, Zhang X, Chen P, Fu Z, Zeng Y, Wang Q, Chipot C, Leggio LL, Sun Y.   +8 more
europepmc   +4 more sources

GH13 Glycogen branching enzymes can adapt the substrate chain length towards their preferences via α-1,4-transglycosylation [PDF]

, 2021
Glycogen branching enzymes (GBEs; 1,4-α-glucan branching enzyme; E.C. 2.4.1.18) have so far been described to be capable of both α-1,6-transglycosylation (branching) and α-1,4-hydrolytic activity. The aim of the present study was to elucidate the mode of
Bax, Hilda Hubertha Maria, Gaenssle, Aline Lucie Odette, Jurak, Edita, van der Maarel, Marc Jos Elise Cornelis   +3 more
core   +1 more source

Proteomic analysis of Bifidobacterium longum subsp. infantis reveals the metabolic insight on consumption of prebiotics and host glycans. [PDF]

, 2013
Bifidobacterium longum subsp. infantis is a common member of the intestinal microbiota in breast-fed infants and capable of metabolizing human milk oligosaccharides (HMO).
An, Hyun Joo, Garrido, Daniel, German, J Bruce, Kim, Jae-Han, Lebrilla, Carlito B, Mills, David A   +5 more
core   +10 more sources

Liver transplantation for type I and type IV glycogen storage disease [PDF]

, 1993
Progressive liver failure or hepatic complications of the primary disease led to orthotopic liver transplantation in eight children with glycogen storage disease over a 9-year period.
A. G. Tzakis, AS Guerra, B Illingworth, B. I. Brown, BI Brown, BI Brown, BI Brown, CA Alper, D Raum, DA Merrill, E. Yunis, GA Bannayan, GB Reed, GM Greene, GT Cori, H Zellweger, HG Hers, HL Greene, IT Ferguson, JJ Malatack, JJ Siekierka, KR McMaster, MW Harding, N Kashiwagi, N Kashiwagi, R. S. Kendall, R. Selby, RC Nordlie, S Servidei, S. Todo, T Ishihara, T. E. Starzl, TE Starl, TE Starzl, TE Starzl, TE Starzl, TE Starzl, V Mazzaferro   +37 more
core   +1 more source

Alpha-1,4-transglycosylation Activity of GH57 Glycogen Branching Enzymes Is Higher in the Absence of a Flexible Loop with a Conserved Tyrosine Residue [PDF]

, 2023
Starch-like polymers can be created through the use of enzymatic modification with glycogen branching enzymes (GBEs). GBEs are categorized in the glycoside hydrolase (GH) family 13 and 57. Both GH13 and GH57 GBEs exhibit branching and hydrolytic activity.
Bax, Hilda Hubertha Maria, Jurak, Edita, van der Maarel, Marc Jos Elise Cornelis   +2 more
core   +1 more source

Metabolic and molecular events occurring during chromoplast biogenesis [PDF]

, 2011
Chromoplasts are nonphotosynthetic plastids that accumulate carotenoids. They derive from other plastid forms, mostly chloroplasts. The biochemical events responsible for the interconversion of one plastid form into another are poorly documented. However,
Barsan, Cristina, Bian, Wanping, Bouzayen, Mondher, Chervin, Christian, Egea, Isabel, Latché, Alain, Pech, Jean-Claude, Purgatto, Eduardo, Zouine, Mohamed   +8 more
core   +2 more sources

Functional and structural characterization of plastidic starch phosphorylase during barley endosperm development [PDF]

, 2017
The production of starch is essential for human nutrition and represents a major metabolic flux in the biosphere. The biosynthesis of starch in storage organs like barley endosperm operates via two main pathways using different substrates: starch ...
Cuesta-Seijo, Jose A., Ruzanski, Christian, Krucewicz, Katarzyna, Meier, Sebastian, Hägglund, Per, Svensson, Birte, Palcic, Monica M.   +6 more
core   +1 more source

Structure of branching enzyme- and amylomaltase modified starch produced from well-defined amylose to amylopectin substrates [PDF]

, 2016
International audienceThermostable branching enzyme (BE, EC 2.4.1.18) from Rhodothermus obamensis in combination with amylomaltase (AM, EC 2.4.1.25) from Thermus thermophilus was used to modify starch structure exploring potentials to extensively ...
Blennow, Andreas, Hamaker, Bruce R., Hebelstrup, Kim H., Jansson, Anita M., Lee, Byung-Hoo, Meier, Sebastian, Rolland-Sabaté, Agnès, Sagnelli, Domenico, Sorndecha, Waraporn, Tongta, Sunanta   +9 more
core   +3 more sources

Assembly of α-Glucan by GlgE and GlgB in Mycobacteria and Streptomycetes [PDF]

, 2016
Actinomycetes, such as mycobacteria and streptomycetes, synthesize α-glucan with α-1,4 linkages and α-1,6 branching to help evade immune responses and to store carbon.
Abdul M. Rashid, Bailey J. M., Cywes C., Farzana Miah, Guzman L. M., Hendrik Koliwer-Brandl, J. Elaine Barclay, Karl Syson, Karol P. Nartowski, Kieser T., Kim C. Findlay, McCleary B. V., Misaki A., Preiss J., Rainer Kalscheuer, Sibyl F. D. Batey, Stephen Bornemann, Summer R., Yaroslav Z. Khimyak   +18 more
core   +2 more sources