p13CMFA: Parsimonious 13C metabolic flux analysis. [PDF]
PLoS Computational Biology, 2019 Deciphering the mechanisms of regulation of metabolic networks subjected to perturbations, including disease states and drug-induced stress, relies on tracing metabolic fluxes.
Carles Foguet +7 more
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Quantitative Metabolomics and Instationary 13C-Metabolic Flux Analysis Reveals Impact of Recombinant Protein Production on Trehalose and Energy Metabolism in Pichia pastoris [PDF]
Metabolites, 2014 Pichia pastoris has been recognized as an effective host for recombinant protein production. In this work, we combine metabolomics and instationary 13C metabolic flux analysis (INST 13C-MFA) using GC-MS and LC-MS/MS to evaluate the potential impact of ...
Joel Jordà +5 more
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13C-metabolic flux analysis reveals metabolic rewiring in HL-60 neutrophil-like cells through differentiation and immune stimulation [PDF]
Metabolic Engineering CommunicationsNeutrophils are innate immune cells and the first line of defense for the maintenance of homeostasis. However, our knowledge of the metabolic rewiring associated with their differentiation and immune stimulation is limited.
Takeo Taniguchi +2 more
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13C-metabolic flux analysis of Saccharomyces cerevisiae in complex media [PDF]
Metabolic Engineering CommunicationsSaccharomyces cerevisiae is often cultivated in complex media for applications in food and other biochemical production. However, 13C-metabolic flux analysis (13C-MFA) has been conducted for S.
Hayato Fujiwara +3 more
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Validation-based model selection for 13C metabolic flux analysis with uncertain measurement errors. [PDF]
PLoS Computational Biology, 2022 Accurate measurements of metabolic fluxes in living cells are central to metabolism research and metabolic engineering. The gold standard method is model-based metabolic flux analysis (MFA), where fluxes are estimated indirectly from mass isotopomer data
Nicolas Sundqvist +5 more
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13C metabolic flux analysis: Classification and characterization from the perspective of mathematical modeling and application in physiological research of neural cell [PDF]
Frontiers in Molecular Neuroscience, 2022 13C metabolic flux analysis (13C-MFA) has emerged as a forceful tool for quantifying in vivo metabolic pathway activity of different biological systems. This technology plays an important role in understanding intracellular metabolism and revealing patho-
Birui Tian +7 more
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Flux-Enabled Exploration of the Role of Sip1 in Galactose Yeast Metabolism [PDF]
Frontiers in Bioengineering and Biotechnology, 2017 13C metabolic flux analysis (13C MFA) is an important systems biology technique that has been used to investigate microbial metabolism for decades. The heterotrimer Snf1 kinase complex plays a key role in the preference Saccharomyces cerevisiae exhibits ...
Christopher M. Shymansky +21 more
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A peptide-based method for 13C Metabolic Flux Analysis in microbial communities. [PDF]
PLoS Computational Biology, 2014 The study of intracellular metabolic fluxes and inter-species metabolite exchange for microbial communities is of crucial importance to understand and predict their behaviour. The most authoritative method of measuring intracellular fluxes, 13C Metabolic
Amit Ghosh +7 more
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A guide to 13C metabolic flux analysis for the cancer biologist [PDF]
Experimental and Molecular Medicine, 2018 Cancer research: tagging to understand cancer metabolism Tracing tagged molecules can help researchers understand the altered metabolism of cancer cells.
Maciek R. Antoniewicz
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Mapping carbon utilization pathways in Histoplasma capsulatum through 13C-metabolic flux analysis [PDF]
mSystemsHistoplasma capsulatum is a human fungal pathogen that survives and proliferates within phagocytic immune cells. To sustain growth in the nutrient-limited phagosome environment, the pathogenic yeast scavenges available carbon sources, which must be ...
Adrian Heckart +5 more
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