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High-resolution 13C metabolic flux analysis
Nature Protocols, 2019Precise quantification of metabolic pathway fluxes in biological systems is of major importance in guiding efforts in metabolic engineering, biotechnology, microbiology, human health, and cell culture. 13C metabolic flux analysis (13C-MFA) is the predominant technique used for determining intracellular fluxes.
Christopher P. Long +1 more
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13C metabolic flux analysis in cell line and bioprocess development
Current Opinion in Chemical Engineering, 2021With an ever-increasing demand for biopharmaceuticals, there is a growing need for high-producing industrial cell lines. 13C metabolic flux analysis provides a comprehensive perspective of host metabolism by rigorously quantifying fluxes within intracellular metabolic networks.
Sarah A Sacco, Jamey D Young
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A Universal Framework for 13C Metabolic Flux Analysis
Metabolic Engineering, 2001A general methodology is presented for the modeling, simulation, design, evaluation, and statistical analysis of (13)C-labeling experiments for metabolic flux analysis. The universal software framework 13C-FLUX was implemented to support all steps of this process.
W, Wiechert +3 more
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Isotopically Nonstationary 13C Metabolic Flux Analysis
2013(13)C metabolic flux analysis (MFA) is a powerful approach for quantifying cell physiology based upon a combination of extracellular flux measurements and intracellular isotope labeling measurements. In this chapter, we present the method of isotopically nonstationary (13)C MFA (INST-MFA), which is applicable to systems that are at metabolic steady ...
Lara J, Jazmin, Jamey D, Young
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Metabolic Engineering, 2001
Metabolic flux analysis using 13C-labeled substrates has become an important tool in metabolic engineering. It allows the detailed quantification of all intracellular fluxes in the central metabolism of a microorganism. The method has strongly evolved in recent years by the introduction of new experimental procedures, measurement techniques, and ...
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Metabolic flux analysis using 13C-labeled substrates has become an important tool in metabolic engineering. It allows the detailed quantification of all intracellular fluxes in the central metabolism of a microorganism. The method has strongly evolved in recent years by the introduction of new experimental procedures, measurement techniques, and ...
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13C Metabolic Flux Analysis: From the Principle to Recent Applications
Current Bioinformatics, 2012Metabolic flux analysis (MFA) has become a fundamental tool for quantifying metabolic pathways, which is essential for in-depth understanding of biological systems. In experimentally based MFA, isotopically labeled substrates are supplied to a biological system and the resulting labeling patterns are analyzed to obtain internal flux information.
Luciana Calheiros Gomes, Manuel Simões
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13C metabolic flux analysis in complex systems
Current Opinion in Biotechnology, 2011Experimental determination of in vivo metabolic rates by methods of (13)C metabolic flux analysis is a pivotal approach to unravel structure and regulation of metabolic networks, in particular with microorganisms grown in minimal media. However, the study of real-life and eukaryotic systems calls for the quantification of fluxes also in cellular ...
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13C-metabolic flux analysis for mevalonate-producing strain of Escherichia coli
Journal of Bioscience and Bioengineering, 2017Mevalonate (MVA) is used to produce various useful products such as drugs, cosmetics and food additives. An MVA-producing strain of Escherichia coli (engineered) was constructed by introducing mvaES genes from Enterococcus faecalis. The engineered strain produced 1.84 mmol/gDCW/h yielding 22% (C-mol/C-mol) of MVA from glucose in the aerobic exponential
Keisuke, Wada +5 more
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