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GC-MS-Based 13C Metabolic Flux Analysis
2014The in vivo analysis of metabolic fluxes has become a valuable method for the investigation of microorganisms. It turned out especially useful in industrial biotechnology for the prediction of beneficial genetic targets for rational strain optimization.
Judith Becker, Christoph Wittmann
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Compartment-specific labeling information in 13C metabolic flux analysis of plants
Phytochemistry, 2007Metabolic engineering of plants has great potential for the low cost production of chemical feedstocks and novel compounds, but to take full advantage of this potential a better understanding of plant central carbon metabolism is needed. Flux studies define the cellular phenotype of living systems and can facilitate rational metabolic engineering ...
Doug K, Allen +2 more
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13C-Metabolic flux analysis of 3T3-L1 adipocytes illuminates its core metabolism under hypoxia
Metabolic Engineering, 2023Hypoxia has been identified as a major factor in the pathogenesis of adipose tissue inflammation, which is a hallmark of obesity and obesity-linked type 2 diabetes mellitus. In this study, we have investigated the impact of hypoxia (1% oxygen) on the physiology and metabolism of 3T3-L1 adipocytes, a widely used cell culture model of adipose ...
Eleanor H. Oates, Maciek R. Antoniewicz
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13C-Metabolic Flux Analysis for Escherichia coli
2014(13)C-Metabolic flux analysis ((13)C-MFA) is used here to study the effects of the knockout of such genes as pgi, zwf, gnd, ppc, pck, pyk, and lpdA on the metabolic changes in Escherichia coli cultivated under aerobic condition. The metabolic regulation mechanisms were clarified by integrating such information as fermentation data, gene expression ...
Yu Matsuoka, Kazuyuki Shimizu
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Current status of 13C-metabolic flux analysis and future perspectives
Process Biochemistry, 2010Abstract Current 13 C-metabolic flux analysis methods were reviewed as well as the weakness of the conventional metabolic flux analysis without 13 C-labeled experiments. Although it has been recognized that 13 C-labeling technique is powerful in estimating the metabolic fluxes, and the program-based flux analysis is necessary, one may not be ...
Yu Matsuoka, Kazuyuki Shimizu
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An Introduction to 13C Metabolic Flux Analysis
2002In recent years, metabolic flux analysis has become one of the major tools in metabolic engineering (1, 2, 3). The aim of MFA is the detailed quantification of all metabolic fluxes in the central metabolic pathways of a microorganism. The result is a flux map that shows the distribution of anabolic and catabolic fluxes over the metabolic network ...
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Synergizing 13C Metabolic Flux Analysis and Metabolic Engineering for Biochemical Production
2017Metabolic engineering of industrial microorganisms to produce chemicals, fuels, and drugs has attracted increasing interest as it provides an environment-friendly and renewable route that does not depend on depleting petroleum sources. However, the microbial metabolism is so complex that metabolic engineering efforts often have difficulty in achieving ...
Weihua, Guo +2 more
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Using Multiple Tracers for 13C Metabolic Flux Analysis
2013(13)C-Metabolic flux analysis ((13)C-MFA) is a powerful technique for quantifying intracellular metabolic fluxes in living cells. These in vivo fluxes provide important information on the physiology of cells in culture, which can be used for metabolic engineering purposes and serve as inputs for systems biology modeling.
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13C metabolic flux analysis: optimal design of isotopic labeling experiments
Current Opinion in Biotechnology, 2013Measuring fluxes by 13C metabolic flux analysis (13C-MFA) has become a key activity in chemical and pharmaceutical biotechnology. Optimal design of isotopic labeling experiments is of central importance to 13C-MFA as it determines the precision with which fluxes can be estimated.
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Phytochemistry, 2007
Metabolic flux analysis is increasingly recognized as an integral component of systems biology. However, techniques for experimental measurement of system-wide metabolic fluxes in purely photoautotrophic systems (growing on CO(2) as the sole carbon source) have not yet been developed due to the unique problems posed by such systems.
Avantika A, Shastri, John A, Morgan
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Metabolic flux analysis is increasingly recognized as an integral component of systems biology. However, techniques for experimental measurement of system-wide metabolic fluxes in purely photoautotrophic systems (growing on CO(2) as the sole carbon source) have not yet been developed due to the unique problems posed by such systems.
Avantika A, Shastri, John A, Morgan
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