Results 21 to 30 of about 35,774 (283)

13CFLUX - third-generation high-performance engine for isotopically (non)stationary 13C metabolic flux analysis. [PDF]

Bioinformatics
Abstract Summary 13C-based metabolic flux analysis (13C-MFA) is a cornerstone of quantitative systems biology, yet its increasing data complexity and methodological diversity place high demands on simulation software.
Stratmann A, Beyß M, Jadebeck JF, Wiechert W, Nöh K.   +4 more
europepmc   +3 more sources

Publishing 13C metabolic flux analysis studies: a review and future perspectives. [PDF]

Metab Eng, 2013
(13)C-Metabolic flux analysis ((13)C-MFA) is a powerful model-based analysis technique for determining intracellular metabolic fluxes in living cells. It has become a standard tool in many labs for quantifying cell physiology, e.g., in metabolic engineering, systems biology, biotechnology, and biomedical research.
Crown SB, Antoniewicz MR.
europepmc   +4 more sources

Mapping Salmonella typhimurium pathways using 13C metabolic flux analysis [PDF]

Metabolic Engineering, 2019
In the last years, Salmonella has been extensively studied not only due to its importance as a pathogen, but also as a host to produce pharmaceutical compounds. However, the full exploitation of Salmonella as a platform for bioproduct delivery has been hampered by the lack of information about its metabolism.
Daniela M, Correia, Cintia R, Sargo, Adilson J, Silva, Sophia T, Santos, Roberto C, Giordano, Eugénio C, Ferreira, Teresa C, Zangirolami, Marcelo P A, Ribeiro, Isabel, Rocha   +8 more
openaire   +2 more sources

Characterization of growth and metabolism of the haloalkaliphile Natronomonas pharaonis [PDF]

, 2010
Natronomonas pharaonis is an archaeon adapted to two extreme conditions: high salt concentration and alkaline pH. It has become one of the model organisms for the study of extremophilic life.
A Varma, AM Feist, AM Feist, B Teusink, BJ Tindall, C Francke, CH Schilling, CM Gomes, D Desmarais, D Oesterhelt, Dieter Oesterhelt, Eduardo Mendoza, F Pfeiffer, Friedhelm Pfeiffer, G Soliman, H Ma, I Schomburg, I Schomburg, J Edwards, J Edwards, J Edwards, JL Reed, LL Grochowski, Locedie Mansueto, M Covert, M Covert, M Falb, M Falb, M Kanehisa, N Price, Nathan D. Price, O Gonzalez, O Gonzalez, Orland Gonzalez, R Hartmann, R Ramakrishna, R Schuetz, R White, Ralf Zimmer, S Schuster, SJ Beard, Tanja Oberwinkler, U Sauer   +42 more
core   +7 more sources

13C MRS: An outstanding tool for metabolic studies [PDF]

, 2005
We provide an overview of 13C magnetic resonance spectroscopy methods and their applications as an established metabolic tool with an emphasis on the use of high-resolution 13C magnetic resonance spectroscopy and 13C isotopomer analysis. Topics addressed
Cerdán, Sebastián, Rodrigues, Tiago B.
core   +1 more source

ScalaFlux: A scalable approach to quantify fluxes in metabolic subnetworks.

PLoS Computational Biology, 2020
13C-metabolic flux analysis (13C-MFA) allows metabolic fluxes to be quantified in living organisms and is a major tool in biotechnology and systems biology.
Pierre Millard, Uwe Schmitt, Patrick Kiefer, Julia A Vorholt, Stéphanie Heux, Jean-Charles Portais   +5 more
doaj   +1 more source

Dynamic 13C Flux Analysis Captures the Reorganization of Adipocyte Glucose Metabolism in Response to Insulin

iScience, 2020
Summary: Cellular metabolism is dynamic, but quantifying non-steady metabolic fluxes by stable isotope tracers presents unique computational challenges.
Lake-Ee Quek, James R. Krycer, Satoshi Ohno, Katsuyuki Yugi, Daniel J. Fazakerley, Richard Scalzo, Sarah D. Elkington, Ziwei Dai, Akiyoshi Hirayama, Satsuki Ikeda, Futaba Shoji, Kumi Suzuki, Jason W. Locasale, Tomoyoshi Soga, David E. James, Shinya Kuroda   +15 more
doaj   +1 more source

Investigation of useful carbon tracers for 13C-metabolic flux analysis of Escherichia coli by considering five experimentally determined flux distributions

Metabolic Engineering Communications, 2016
The 13C-MFA experiments require an optimal design since the precision or confidence intervals of the estimated flux levels depends on factors such as the composition of 13C-labeled carbon sources, as well as the metabolic flux distribution of interest ...
Kousuke Maeda, Nobuyuki Okahashi, Yoshihiro Toya, Fumio Matsuda, Hiroshi Shimizu   +4 more
doaj   +1 more source

13C-assisted metabolic flux analysis to investigate heterotrophic and mixotrophic metabolism in Cupriavidus necator H16 [PDF]

, 2018
Introduction. Cupriavidus necator H16 is a gram-negative bacterium, capable of lithoautotrophic growth by utilizing hydrogen as an energy source and fixing carbon dioxide (CO2) through Calvin-Benson-Bassham (CBB) cycle. The potential to utilize synthesis
A Pohlmann, A Steinbüchel, B Bowien, B Christensen, C Wittmann, CG Friedrich, CH Poskar, CJ Brigham, E Schwartz, E Schwartz, E Wilde, HG Schlegel, HG Schlegel, JD Young, JL Ienczak, JM Park, K Peplinski, K Walsh, K-H Tang, L Stryer, LE Quek, M Lopar, M Raberg, M Weitzel, MR Antoniewicz, N Bruland, N Tanadchangsaeng, N Zamboni, N Zamboni, Naglis Malys, Nigel P. Minton, P Millard, R Shimizu, R Shimizu, S Alagesan, Swathi Alagesan, T Fukui, W Wiechert, W Wiechert, X Feng   +39 more
core   +2 more sources

BCKDH: the missing link in apicomplexan mitochondrial metabolism is required for full virulence of Toxoplasma gondii and Plasmodium berghei [PDF]

, 2014
While the apicomplexan parasites Plasmodium falciparum and Toxoplasma gondii are thought to primarily depend on glycolysis for ATP synthesis, recent studies have shown that they can fully catabolize glucose in a canonical TCA cycle.
Barrett, Michael P., Billker, Oliver, Creek, Darren J., Limenitakis, Julien, Macrae, James I., McConville, Malcolm J., Modrzynska, Katarzyna K., Oppenheim, Rebecca D., Pino, Paco, Polonais, Valerie, Seeber, Frank, Soldati-Favre, Dominique   +11 more
core   +1 more source