Results 31 to 40 of about 35,774 (283)
Metabolites, 2014 13C metabolic flux analysis (MFA) is a tool of metabolic engineering for investigation of in vivo flux distribution. A direct 13C enrichment analysis of intracellular free amino acids (FAAs) is expected to reduce time for labeling experiments of the MFA.
Nobuyuki Okahashi +3 more
doaj +1 more source
mSystems, 2020 In Ralstonia solanacearum, a devastating phytopathogen whose metabolism is poorly understood, we observed that the Entner-Doudoroff (ED) pathway and nonoxidative pentose phosphate pathway (non-OxPPP) bypass glycolysis and OxPPP under glucose oxidation ...
Poonam Jyoti +6 more
doaj +1 more source
Systems biology analysis of drivers underlying hallmarks of cancer cell metabolism. [PDF]
, 2017 Malignant transformation is often accompanied by significant metabolic changes. To identify drivers underlying these changes, we calculated metabolic flux states for the NCI60 cell line collection and correlated the variance between metabolic states of ...
Bordbar, Aarash +5 more
core +1 more source
Bio-Protocol, 2023 The flux in photosynthesis can be studied by performing 13CO2 pulse labelling and analysing the temporal labelling kinetics of metabolic intermediates using gas or liquid chromatography linked to mass spectrometry.
Or Geffen, David Achaintre, Haim Treves
doaj +1 more source
HIF1A reduces acute lung injury by optimizing carbohydrate metabolism in the alveolar epithelium [PDF]
, 2013 Background: While acute lung injury (ALI) contributes significantly to critical illness, it resolves spontaneously in many instances. The majority of patients experiencing ALI require mechanical ventilation.
Bonney, Megan +9 more
core +3 more sources
BMC Biology, 2019 The study of tumorigenic rewiring of metabolic flux is at the heart of cancer metabolic research. Here, we review two widely used computational flux inference approaches: isotope tracing coupled with Metabolic Flux Analysis (13C-MFA) and COnstraint-Based
Shoval Lagziel +2 more
doaj +1 more source
Rapid Prediction of Bacterial Heterotrophic Fluxomics Using Machine Learning and Constraint Programming. [PDF]
PLoS Computational Biology, 2016 13C metabolic flux analysis (13C-MFA) has been widely used to measure in vivo enzyme reaction rates (i.e., metabolic flux) in microorganisms. Mining the relationship between environmental and genetic factors and metabolic fluxes hidden in existing ...
Stephen Gang Wu +8 more
doaj +1 more source
Fructose metabolism in Chromohalobacter salexigens: interplay between the Embden–Meyerhof–Parnas and Entner–Doudoroff pathways [PDF]
, 2019 Background The halophilic bacterium Chromohalobacter salexigens metabolizes glucose exclusively through the Entner–Doudoroff (ED) pathway, an adaptation which results in inefficient growth, with significant carbon overflow, especially at low salinity ...
Argandoña Bertrán, Montserrat +12 more
core +1 more source
Fluxomers: a new approach for 13C metabolic flux analysis [PDF]
BMC Systems Biology, 2011 Abstract Background The ability to perform quantitative studies using isotope tracers and metabolic flux analysis (MFA) is critical for detecting pathway bottlenecks and elucidating network regulation in biological systems, especially those that have been engineered to alter their native metabolic capacities ...
Srour, Orr +2 more
openaire +2 more sources
Two-Scale 13C Metabolic Flux Analysis for Metabolic Engineering [PDF]
, 2018 Accelerating the Design-Build-Test-Learn (DBTL) cycle in synthetic biology is critical to achieving rapid and facile bioengineering of organisms for the production of, e.g., biofuels and other chemicals. The Learn phase involves using data obtained from the Test phase to inform the next Design phase.
Ando, David, Garcia Martin, Hector
openaire +4 more sources