Results 241 to 250 of about 35,777 (291)

Phase-driven rewiring in Escherichia coli enhances coenzyme Q10 biosynthesis via temporal and energetic coordination. [PDF]

Appl Microbiol Biotechnol
Li H, Wu Y, Qin D, Xie J, Huang W, Chen R, Liu H, Wang Y, Zhao D.   +8 more
europepmc   +1 more source

Inferring the Metabolic Objectives of Mammalian Cells via Inverse Modeling of Fluxomics and Metabolomics

Morrissey J, Monteiro M, Betenbaugh M, Kontoravdi C.   +3 more
europepmc   +1 more source

Some of the next articles are maybe not open access.

Related searches:

In vivo 2H/13C flux analysis in metabolism research

Current Opinion in Biotechnology, 2021
Identifying the factors and mechanisms that regulate metabolism under normal and diseased states requires methods to quantify metabolic fluxes of live tissues within their physiological milieu. A number of recent developments have expanded the reach and depth of isotope-based in vivo flux analysis, which have in turn challenged existing dogmas in ...
Tomasz K Bednarski, Mohsin Rahim, Jamey D Young   +2 more
openaire   +2 more sources

High-resolution 13C metabolic flux analysis

Nature Protocols, 2019
Precise quantification of metabolic pathway fluxes in biological systems is of major importance in guiding efforts in metabolic engineering, biotechnology, microbiology, human health, and cell culture. 13C metabolic flux analysis (13C-MFA) is the predominant technique used for determining intracellular fluxes.
Christopher P. Long, Maciek R. Antoniewicz   +1 more
openaire   +2 more sources

A Universal Framework for 13C Metabolic Flux Analysis

Metabolic Engineering, 2001
A general methodology is presented for the modeling, simulation, design, evaluation, and statistical analysis of (13)C-labeling experiments for metabolic flux analysis. The universal software framework 13C-FLUX was implemented to support all steps of this process.
W, Wiechert, M, Möllney, S, Petersen, A A, de Graaf   +3 more
openaire   +2 more sources

Isotopically Nonstationary 13C Metabolic Flux Analysis

2013
(13)C metabolic flux analysis (MFA) is a powerful approach for quantifying cell physiology based upon a combination of extracellular flux measurements and intracellular isotope labeling measurements. In this chapter, we present the method of isotopically nonstationary (13)C MFA (INST-MFA), which is applicable to systems that are at metabolic steady ...
Lara J, Jazmin, Jamey D, Young
openaire   +2 more sources

13C Metabolic Flux Analysis

Metabolic Engineering, 2001
Metabolic flux analysis using 13C-labeled substrates has become an important tool in metabolic engineering. It allows the detailed quantification of all intracellular fluxes in the central metabolism of a microorganism. The method has strongly evolved in recent years by the introduction of new experimental procedures, measurement techniques, and ...
openaire   +2 more sources

13C flux analysis of cyanobacterial metabolism

Photosynthesis Research, 2014
(13)C metabolic flux analysis (MFA) has made important contributions to our understanding of the physiology of model strains of E. coli and yeast, and it has been widely used to guide metabolic engineering efforts in these microorganisms. Recent advancements in (13)C MFA methodology combined with publicly available software tools are creating new ...
Adeola O, Adebiyi, Lara J, Jazmin, Jamey D, Young   +2 more
openaire   +2 more sources

13C metabolic flux analysis in complex systems

Current Opinion in Biotechnology, 2011
Experimental determination of in vivo metabolic rates by methods of (13)C metabolic flux analysis is a pivotal approach to unravel structure and regulation of metabolic networks, in particular with microorganisms grown in minimal media. However, the study of real-life and eukaryotic systems calls for the quantification of fluxes also in cellular ...
openaire   +2 more sources

13C-Metabolic flux analysis of 3T3-L1 adipocytes illuminates its core metabolism under hypoxia

Metabolic Engineering, 2023
Hypoxia has been identified as a major factor in the pathogenesis of adipose tissue inflammation, which is a hallmark of obesity and obesity-linked type 2 diabetes mellitus. In this study, we have investigated the impact of hypoxia (1% oxygen) on the physiology and metabolism of 3T3-L1 adipocytes, a widely used cell culture model of adipose ...
Eleanor H. Oates, Maciek R. Antoniewicz
openaire   +2 more sources