Results 251 to 260 of about 35,777 (291)

13C metabolic flux analysis in cell line and bioprocess development

Current Opinion in Chemical Engineering, 2021
With an ever-increasing demand for biopharmaceuticals, there is a growing need for high-producing industrial cell lines. 13C metabolic flux analysis provides a comprehensive perspective of host metabolism by rigorously quantifying fluxes within intracellular metabolic networks.
Sarah A Sacco, Jamey D Young
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13C Metabolic Flux Analysis: From the Principle to Recent Applications

Current Bioinformatics, 2012
Metabolic flux analysis (MFA) has become a fundamental tool for quantifying metabolic pathways, which is essential for in-depth understanding of biological systems. In experimentally based MFA, isotopically labeled substrates are supplied to a biological system and the resulting labeling patterns are analyzed to obtain internal flux information.
Luciana Calheiros Gomes, Manuel Simões
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GC-MS-Based 13C Metabolic Flux Analysis

2014
The in vivo analysis of metabolic fluxes has become a valuable method for the investigation of microorganisms. It turned out especially useful in industrial biotechnology for the prediction of beneficial genetic targets for rational strain optimization.
Judith Becker, Christoph Wittmann
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An Introduction to 13C Metabolic Flux Analysis

2002
In recent years, metabolic flux analysis has become one of the major tools in metabolic engineering (1, 2, 3). The aim of MFA is the detailed quantification of all metabolic fluxes in the central metabolic pathways of a microorganism. The result is a flux map that shows the distribution of anabolic and catabolic fluxes over the metabolic network ...
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Synergizing 13C Metabolic Flux Analysis and Metabolic Engineering for Biochemical Production

2017
Metabolic engineering of industrial microorganisms to produce chemicals, fuels, and drugs has attracted increasing interest as it provides an environment-friendly and renewable route that does not depend on depleting petroleum sources. However, the microbial metabolism is so complex that metabolic engineering efforts often have difficulty in achieving ...
Weihua, Guo, Jiayuan, Sheng, Xueyang, Feng   +2 more
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13C-metabolic flux analysis for mevalonate-producing strain of Escherichia coli

Journal of Bioscience and Bioengineering, 2017
Mevalonate (MVA) is used to produce various useful products such as drugs, cosmetics and food additives. An MVA-producing strain of Escherichia coli (engineered) was constructed by introducing mvaES genes from Enterococcus faecalis. The engineered strain produced 1.84 mmol/gDCW/h yielding 22% (C-mol/C-mol) of MVA from glucose in the aerobic exponential
Keisuke, Wada, Yoshihiro, Toya, Satomi, Banno, Katsunori, Yoshikawa, Fumio, Matsuda, Hiroshi, Shimizu   +5 more
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Compartment-specific labeling information in 13C metabolic flux analysis of plants

Phytochemistry, 2007
Metabolic engineering of plants has great potential for the low cost production of chemical feedstocks and novel compounds, but to take full advantage of this potential a better understanding of plant central carbon metabolism is needed. Flux studies define the cellular phenotype of living systems and can facilitate rational metabolic engineering ...
Doug K, Allen, Yair, Shachar-Hill, John B, Ohlrogge   +2 more
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13C-Metabolic Flux Analysis for Escherichia coli

2014
(13)C-Metabolic flux analysis ((13)C-MFA) is used here to study the effects of the knockout of such genes as pgi, zwf, gnd, ppc, pck, pyk, and lpdA on the metabolic changes in Escherichia coli cultivated under aerobic condition. The metabolic regulation mechanisms were clarified by integrating such information as fermentation data, gene expression ...
Yu Matsuoka, Kazuyuki Shimizu
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Using Multiple Tracers for 13C Metabolic Flux Analysis

2013
(13)C-Metabolic flux analysis ((13)C-MFA) is a powerful technique for quantifying intracellular metabolic fluxes in living cells. These in vivo fluxes provide important information on the physiology of cells in culture, which can be used for metabolic engineering purposes and serve as inputs for systems biology modeling.
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Parallel labeling experiments with [U-13C]glucose validate E. coli metabolic network model for 13C metabolic flux analysis

Metabolic Engineering, 2012
(13)C-metabolic flux analysis (MFA) is a widely used method for measuring intracellular metabolic fluxes in living cells. (13)C MFA relies on several key assumptions: (1) the assumed metabolic network model is complete, in that it accounts for all significant enzymatic and transport reactions; (2) (13)C-labeling measurements are accurate and precise ...
Robert W, Leighty, Maciek R, Antoniewicz
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