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13C-Based Metabolic Flux Analysis: Fundamentals and Practice
2013Isotope-based metabolic flux analysis is one of the emerging technologies applied to system level metabolic phenotype characterization in metabolic engineering. Among the developed approaches, (13)C-based metabolic flux analysis has been established as a standard tool and has been widely applied to quantitative pathway characterization of diverse ...
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13C metabolic flux analysis: optimal design of isotopic labeling experiments
Current Opinion in Biotechnology, 2013Measuring fluxes by 13C metabolic flux analysis (13C-MFA) has become a key activity in chemical and pharmaceutical biotechnology. Optimal design of isotopic labeling experiments is of central importance to 13C-MFA as it determines the precision with which fluxes can be estimated.
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Biotekhnologiya, 2017
At present, 13C-MFA is a primary method for quantitatively characterizing intracellular carbon fluxes in cells in vivo under steady-state conditions. The method has been successfully used to investigate both the fundamental characteristics of prokaryotic and eukaryotic cell metabolism and to improve producer strains for more than twenty years.
L. I. Golubeva +2 more
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At present, 13C-MFA is a primary method for quantitatively characterizing intracellular carbon fluxes in cells in vivo under steady-state conditions. The method has been successfully used to investigate both the fundamental characteristics of prokaryotic and eukaryotic cell metabolism and to improve producer strains for more than twenty years.
L. I. Golubeva +2 more
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13C-Based Metabolic Flux Analysis of Recombinant Pichia pastoris
2014Overexpression of a foreign protein may negatively affect several cell growth parameters, as well as cause cellular stress. Central (or core) metabolism plays a crucial role since it supplies energy, reduction equivalents, and precursor molecules for the recombinant product, cell's maintenance, and growth needs.
Pau Ferrer, Joan Albiol
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Current status of 13C-metabolic flux analysis and future perspectives
Process Biochemistry, 2010Abstract Current 13 C-metabolic flux analysis methods were reviewed as well as the weakness of the conventional metabolic flux analysis without 13 C-labeled experiments. Although it has been recognized that 13 C-labeling technique is powerful in estimating the metabolic fluxes, and the program-based flux analysis is necessary, one may not be ...
Yu Matsuoka, Kazuyuki Shimizu
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13C metabolic flux analysis of recombinant expression hosts
Current Opinion in Biotechnology, 2014Identifying host cell metabolic phenotypes that promote high recombinant protein titer is a major goal of the biotech industry. (13)C metabolic flux analysis (MFA) provides a rigorous approach to quantify these metabolic phenotypes by applying isotope tracers to map the flow of carbon through intracellular metabolic pathways.
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Neurochemistry International, 2008
In this work, brain cell metabolism was investigated by (13)C NMR spectroscopy and metabolic flux analysis (MFA). Monotypic cultures of astrocytes were incubated with labeled glucose for 38 h, and the distribution of the label was analyzed by (13)C NMR spectroscopy.
Ana P, Teixeira +4 more
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In this work, brain cell metabolism was investigated by (13)C NMR spectroscopy and metabolic flux analysis (MFA). Monotypic cultures of astrocytes were incubated with labeled glucose for 38 h, and the distribution of the label was analyzed by (13)C NMR spectroscopy.
Ana P, Teixeira +4 more
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Modelling of Neural Metabolism Using 13C-NMR Spectroscopy and Metabolic Flux Analysis
2010A metabolic flux analysis (MFA) study was performed to infer about the metabolic plasticity of astrocytes when exposed to two environmental stresses. In the first study an astrocytic culture was subjected to a hypoglycemic period of 24 h and the metabolic response was investigated during and after the insult.
Ana P. Teixeira +6 more
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Bile acids and the gut microbiota: metabolic interactions and impacts on disease
Nature Reviews Microbiology, 2022Stephanie Collins, Andrew D Patterson
exaly