Results 21 to 30 of about 35,777 (291)

13C MRS: An outstanding tool for metabolic studies [PDF]

, 2005
We provide an overview of 13C magnetic resonance spectroscopy methods and their applications as an established metabolic tool with an emphasis on the use of high-resolution 13C magnetic resonance spectroscopy and 13C isotopomer analysis. Topics addressed
Cerdán, Sebastián, Rodrigues, Tiago B.
core   +1 more source

ScalaFlux: A scalable approach to quantify fluxes in metabolic subnetworks.

PLoS Computational Biology, 2020
13C-metabolic flux analysis (13C-MFA) allows metabolic fluxes to be quantified in living organisms and is a major tool in biotechnology and systems biology.
Pierre Millard, Uwe Schmitt, Patrick Kiefer, Julia A Vorholt, Stéphanie Heux, Jean-Charles Portais   +5 more
doaj   +1 more source

Dynamic 13C Flux Analysis Captures the Reorganization of Adipocyte Glucose Metabolism in Response to Insulin

iScience, 2020
Summary: Cellular metabolism is dynamic, but quantifying non-steady metabolic fluxes by stable isotope tracers presents unique computational challenges.
Lake-Ee Quek, James R. Krycer, Satoshi Ohno, Katsuyuki Yugi, Daniel J. Fazakerley, Richard Scalzo, Sarah D. Elkington, Ziwei Dai, Akiyoshi Hirayama, Satsuki Ikeda, Futaba Shoji, Kumi Suzuki, Jason W. Locasale, Tomoyoshi Soga, David E. James, Shinya Kuroda   +15 more
doaj   +1 more source

SUMOFLUX: A Generalized Method for Targeted 13C Metabolic Flux Ratio Analysis. [PDF]

PLoS Computational Biology, 2016
Metabolic fluxes are a cornerstone of cellular physiology that emerge from a complex interplay of enzymes, carriers, and nutrients. The experimental assessment of in vivo intracellular fluxes using stable isotopic tracers is essential if we are to ...
Maria Kogadeeva, Nicola Zamboni
doaj   +1 more source

Investigation of useful carbon tracers for 13C-metabolic flux analysis of Escherichia coli by considering five experimentally determined flux distributions

Metabolic Engineering Communications, 2016
The 13C-MFA experiments require an optimal design since the precision or confidence intervals of the estimated flux levels depends on factors such as the composition of 13C-labeled carbon sources, as well as the metabolic flux distribution of interest ...
Kousuke Maeda, Nobuyuki Okahashi, Yoshihiro Toya, Fumio Matsuda, Hiroshi Shimizu   +4 more
doaj   +1 more source

13C-assisted metabolic flux analysis to investigate heterotrophic and mixotrophic metabolism in Cupriavidus necator H16 [PDF]

, 2018
Introduction. Cupriavidus necator H16 is a gram-negative bacterium, capable of lithoautotrophic growth by utilizing hydrogen as an energy source and fixing carbon dioxide (CO2) through Calvin-Benson-Bassham (CBB) cycle. The potential to utilize synthesis
A Pohlmann, A Steinbüchel, B Bowien, B Christensen, C Wittmann, CG Friedrich, CH Poskar, CJ Brigham, E Schwartz, E Schwartz, E Wilde, HG Schlegel, HG Schlegel, JD Young, JL Ienczak, JM Park, K Peplinski, K Walsh, K-H Tang, L Stryer, LE Quek, M Lopar, M Raberg, M Weitzel, MR Antoniewicz, N Bruland, N Tanadchangsaeng, N Zamboni, N Zamboni, Naglis Malys, Nigel P. Minton, P Millard, R Shimizu, R Shimizu, S Alagesan, Swathi Alagesan, T Fukui, W Wiechert, W Wiechert, X Feng   +39 more
core   +2 more sources

Two-Scale 13C Metabolic Flux Analysis for Metabolic Engineering [PDF]

, 2018
Accelerating the Design-Build-Test-Learn (DBTL) cycle in synthetic biology is critical to achieving rapid and facile bioengineering of organisms for the production of, e.g., biofuels and other chemicals. The Learn phase involves using data obtained from the Test phase to inform the next Design phase.
Ando, David, Garcia Martin, Hector
openaire   +4 more sources

BCKDH: the missing link in apicomplexan mitochondrial metabolism is required for full virulence of Toxoplasma gondii and Plasmodium berghei [PDF]

, 2014
While the apicomplexan parasites Plasmodium falciparum and Toxoplasma gondii are thought to primarily depend on glycolysis for ATP synthesis, recent studies have shown that they can fully catabolize glucose in a canonical TCA cycle.
Barrett, Michael P., Billker, Oliver, Creek, Darren J., Limenitakis, Julien, Macrae, James I., McConville, Malcolm J., Modrzynska, Katarzyna K., Oppenheim, Rebecca D., Pino, Paco, Polonais, Valerie, Seeber, Frank, Soldati-Favre, Dominique   +11 more
core   +1 more source

Reliable Metabolic Flux Estimation in Escherichia coli Central Carbon Metabolism Using Intracellular Free Amino Acids

Metabolites, 2014
13C metabolic flux analysis (MFA) is a tool of metabolic engineering for investigation of in vivo flux distribution. A direct 13C enrichment analysis of intracellular free amino acids (FAAs) is expected to reduce time for labeling experiments of the MFA.
Nobuyuki Okahashi, Shuichi Kajihata, Chikara Furusawa, Hiroshi Shimizu   +3 more
doaj   +1 more source

Mapping Salmonella typhimurium pathways using 13C metabolic flux analysis [PDF]

Metabolic Engineering, 2019
In the last years, Salmonella has been extensively studied not only due to its importance as a pathogen, but also as a host to produce pharmaceutical compounds. However, the full exploitation of Salmonella as a platform for bioproduct delivery has been hampered by the lack of information about its metabolism.
Daniela M, Correia, Cintia R, Sargo, Adilson J, Silva, Sophia T, Santos, Roberto C, Giordano, Eugénio C, Ferreira, Teresa C, Zangirolami, Marcelo P A, Ribeiro, Isabel, Rocha   +8 more
openaire   +2 more sources