Results 41 to 50 of about 35,777 (291)
Serine one-carbon catabolism with formate overflow [PDF]
, 2016 Serine catabolism to glycine and a one-carbon unit has been linked to the anabolic requirements of proliferating mammalian cells. However, genome-scale modeling predicts a catabolic role with one-carbon release as formate. We experimentally prove that in
Athineos, Dimitris +11 more
core +1 more source
Stable isotope metabolomics of pulmonary artery smooth muscle and endothelial cells in pulmonary hypertension and with TGF-beta treatment. [PDF]
, 2020 Altered metabolism in pulmonary artery smooth muscle cells (PASMCs) and endothelial cells (PAECs) contributes to the pathology of pulmonary hypertension (PH), but changes in substrate uptake and how substrates are utilized have not been fully ...
D' Alessandro, Angelo +12 more
core +1 more source
Fluxomers: a new approach for 13C metabolic flux analysis [PDF]
BMC Systems Biology, 2011 Abstract Background The ability to perform quantitative studies using isotope tracers and metabolic flux analysis (MFA) is critical for detecting pathway bottlenecks and elucidating network regulation in biological systems, especially those that have been engineered to alter their native metabolic capacities ...
Srour, Orr +2 more
openaire +2 more sources
Cmah-dystrophin deficient mdx mice display an accelerated cardiac phenotype that is improved following peptide-PMO exon skipping treatment [PDF]
, 2018 Duchenne muscular dystrophy (DMD) is caused by loss of dystrophin protein, leading to progressive muscle weakness and premature death due to respiratory and/or cardiac complications.
Ball, V +15 more
core +3 more sources
BMC Bioinformatics, 2017 Background Modeling of microbial metabolism is a topic of growing importance in biotechnology. Mathematical modeling helps provide a mechanistic understanding for the studied process, separating the main drivers from the circumstantial ones, bounding the
Garrett W. Birkel +8 more
doaj +1 more source
Imaging glutathione depletion in the rat brain using ascorbate-derived hyperpolarized MR and PET probes. [PDF]
, 2018 Oxidative stress is a critical feature of several common neurologic disorders. The brain is well adapted to neutralize oxidative injury by maintaining a high steady-state concentration of small-molecule intracellular antioxidants including glutathione in
Carroll, Valerie N +10 more
core +1 more source
13C-based metabolic flux analysis
Nature Protocols, 2009 Stable isotope, and in particular (13)C-based flux analysis, is the exclusive approach to experimentally quantify the integrated responses of metabolic networks. Here we describe a protocol that is based on growing microbes on (13)C-labeled glucose and subsequent gas chromatography mass spectrometric detection of (13)C-patterns in protein-bound amino ...
Zamboni, Nicola +3 more
openaire +4 more sources
Bioengineering, 2015 Metabolic engineering of various industrial microorganisms to produce chemicals, fuels, and drugs has raised interest since it is environmentally friendly, sustainable, and independent of nonrenewable resources.
Weihua Guo, Jiayuan Sheng, Xueyang Feng
doaj +1 more source
Hyperpolarized 13C-pyruvate MRI detects real-time metabolic flux in prostate cancer metastases to bone and liver: a clinical feasibility study. [PDF]
, 2020 BackgroundHyperpolarized (HP) 13C-pyruvate MRI is a stable-isotope molecular imaging modality that provides real-time assessment of the rate of metabolism through glycolytic pathways in human prostate cancer.
Aggarwal, Rahul +13 more
core
Updates in metabolomics tools and resources: 2014-2015 [PDF]
, 2016 Data processing and interpretation represent the most challenging and time-consuming steps in high-throughput metabolomic experiments, regardless of the analytical platforms (MS or NMR spectroscopy based) used for data acquisition.
Misra, Biswapriya B. +1 more
core +1 more source