Results 1 to 10 of about 712,979 (385)

A comparison of 16S rRNA-gene and 16S rRNA-transcript derived microbial communities in bulk and rhizosphere soils [PDF]

Frontiers in Microbiology
Root exudates in a plant’s rhizosphere alters microbial community membership and activity, which can in turn alter a plant’s health and fitness. In this study we characterized bacterial community composition, using 16S rRNA-gene (DNA) sequencing to ...
Alessandra Ceretto, Alessandra Ceretto, Cynthia Weinig, Cynthia Weinig, Cynthia Weinig   +4 more
doaj   +2 more sources

Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations [PDF]

Applied and Environmental Microbiology, 1990
Rudolf Amann, Brian J. Binder, Robert Olson, Sallie W. Chisholm, Richard Devereux, David A. Stahl   +5 more
openalex   +2 more sources

16S-ITS-23S rRNA operon segment sequencing provides necessary and sufficient conditions for bacterial species-specific identification

Инфекция и иммунитет, 2022
Introduction. Sequencing of the 16S rRNA gene is the predominant method for assessing microbial communities and strain molecular identification. The short reads (2nd generation sequencing)-based technology does not allow analysis beyond the 16S rRNA gene.
O. B. Ogarkov, S. N. Zhdanova, E. A. Orlova, P. A. Khromova, N. L. Belkova, V. V. Sinkov, I. G. Kondratov   +6 more
doaj   +1 more source

16S-FASAS: an integrated pipeline for synthetic full-length 16S rRNA gene sequencing data analysis [PDF]

PeerJ, 2022
Background The full-length 16S rRNA sequencing can better improve the taxonomic and phylogenetic resolution compared to the partial 16S rRNA gene sequencing.
Ke Zhang, Rongnan Lin, Yujun Chang, Qing Zhou, Zhi Zhang   +4 more
doaj   +2 more sources

NGS in the clinical microbiology settings

Frontiers in Cellular and Infection Microbiology, 2022
We hypothesized that targeted NGS sequencing might have an advantage over Sanger sequencing, especially in polymicrobial infections. The study included 55 specimens from 51 patients.
Milena Pitashny, Milena Pitashny, Balqees Kadry, Raya Shalaginov, Liat Gazit, Yaniv Zohar, Moran Szwarcwort, Yoav Stabholz, Mical Paul   +8 more
doaj   +1 more source

Description of larvae of Eleotris perniger (Teleostei: Eleotridae) in transition from saltwater to freshwater from Montserrat, West Indies

Novitates Caribaea, 2021
Larvae of the Smallscaled Spinycheek Sleeper (Eleotris perniger) in transition from saltwater to freshwater were identified using COI and 16S gene sequences and the morphology and color pattern were then described.
Robert E. Schmidt, Erin McMullin, Jeremy J. Wright, Bryan Weatherwax   +3 more
doaj   +1 more source

DNA barcoding uncovers cryptic diversity in 50% of deep-sea Antarctic polychaetes [PDF]

Royal Society Open Science, 2016
The Antarctic marine environment is a diverse ecosystem currently experiencing some of the fastest rates of climatic change. The documentation and management of these changes requires accurate estimates of species diversity.
Madeleine J. Brasier, Helena Wiklund, Lenka Neal, Rachel Jeffreys, Katrin Linse, Henry Ruhl, Adrian G. Glover   +6 more
doaj   +1 more source

Species Identification and Profiling of Complex Microbial Communities Using Shotgun Illumina Sequencing of 16S rRNA Amplicon Sequences [PDF]

, 2013
The high throughput and cost-effectiveness afforded by short-read sequencing technologies, in principle, enable researchers to perform 16S rRNA profiling of complex microbial communities at unprecedented depth and resolution. Existing Illumina sequencing
Hibberd, Martin Lloyd, Ho, Eliza Xin Pei, Kukkillaya, Vinutha Uppoor, Lay, Christophe, Low, Louie, Nagarajan, Niranjan, Ong, Swee Hoe, Wilm, Andreas   +7 more
core   +9 more sources

The Pattern Recognition Receptor FLS2 Can Shape the Arabidopsis Rhizosphere Microbiome β-Diversity but Not EFR1 and CERK1

Plants, 2022
Pathogen associated molecular pattern (PAMP) triggered immunity (PTI) is the first line of plant defense. We hypothesized that the absence of pattern recognition receptors (PRRs) in plants could influence the rhizosphere microbiome.
Jose P. Fonseca, Venkatachalam Lakshmanan, Clarissa Boschiero, Kirankumar S. Mysore   +3 more
doaj   +1 more source

Comparison between 16S rRNA and shotgun sequencing data for the taxonomic characterization of the gut microbiota

Scientific Reports, 2021
In this paper we compared taxonomic results obtained by metataxonomics (16S rRNA gene sequencing) and metagenomics (whole shotgun metagenomic sequencing) to investigate their reliability for bacteria profiling, studying the chicken gut as a model system.
Francesco Durazzi, C. Sala, G. Castellani, G. Manfreda, D. Remondini, A. De Cesare   +5 more
semanticscholar   +1 more source