Results 1 to 10 of about 267,584 (295)

Detection of Pathogens by a Novel User-Developed Broad-Range BR 16S PCR rRNA Polymerase Chain Reaction/Gene Sequencing Assay: Multiyear Experience in a Large Canadian Healthcare Zone. [PDF]

Microorganisms
Between 2015 and 2022, we evaluated a novel broad-range (BR) 16S PCR rDNA PCR/Sanger sequencing assay to improve diagnosis of invasive infections in culture-negative specimens.
Griener T, Chow B, Church D.
europepmc   +2 more sources

16S-ITS-23S rRNA operon segment sequencing provides necessary and sufficient conditions for bacterial species-specific identification

Инфекция и иммунитет, 2022
Introduction. Sequencing of the 16S rRNA gene is the predominant method for assessing microbial communities and strain molecular identification. The short reads (2nd generation sequencing)-based technology does not allow analysis beyond the 16S rRNA gene.
O. B. Ogarkov, S. N. Zhdanova, E. A. Orlova, P. A. Khromova, N. L. Belkova, V. V. Sinkov, I. G. Kondratov   +6 more
doaj   +1 more source

16S-FASAS: an integrated pipeline for synthetic full-length 16S rRNA gene sequencing data analysis [PDF]

PeerJ, 2022
Background The full-length 16S rRNA sequencing can better improve the taxonomic and phylogenetic resolution compared to the partial 16S rRNA gene sequencing.
Ke Zhang, Rongnan Lin, Yujun Chang, Qing Zhou, Zhi Zhang   +4 more
doaj   +2 more sources

Interleukin-16 [PDF]

Journal of Leukocyte Biology, 1997
Abstract Interleukin 16 (IL-16) was initially described in 1982 as the first T cell chemoattractant. Through interaction with CD4, IL-16 has now been characterized as a chemoattractant for a variety of CD4+ immune cells. Recent in vivo studies have more fully characterized IL-16 as an immunom  odulatory cytokine that contributes to the ...
W W, Cruikshank, H, Kornfeld, D M, Center   +2 more
openaire   +3 more sources

NGS in the clinical microbiology settings

Frontiers in Cellular and Infection Microbiology, 2022
We hypothesized that targeted NGS sequencing might have an advantage over Sanger sequencing, especially in polymicrobial infections. The study included 55 specimens from 51 patients.
Milena Pitashny, Milena Pitashny, Balqees Kadry, Raya Shalaginov, Liat Gazit, Yaniv Zohar, Moran Szwarcwort, Yoav Stabholz, Mical Paul   +8 more
doaj   +1 more source

Description of larvae of Eleotris perniger (Teleostei: Eleotridae) in transition from saltwater to freshwater from Montserrat, West Indies

Novitates Caribaea, 2021
Larvae of the Smallscaled Spinycheek Sleeper (Eleotris perniger) in transition from saltwater to freshwater were identified using COI and 16S gene sequences and the morphology and color pattern were then described.
Robert E. Schmidt, Erin McMullin, Jeremy J. Wright, Bryan Weatherwax   +3 more
doaj   +1 more source

DNA barcoding uncovers cryptic diversity in 50% of deep-sea Antarctic polychaetes [PDF]

Royal Society Open Science, 2016
The Antarctic marine environment is a diverse ecosystem currently experiencing some of the fastest rates of climatic change. The documentation and management of these changes requires accurate estimates of species diversity.
Madeleine J. Brasier, Helena Wiklund, Lenka Neal, Rachel Jeffreys, Katrin Linse, Henry Ruhl, Adrian G. Glover   +6 more
doaj   +1 more source

The Pattern Recognition Receptor FLS2 Can Shape the Arabidopsis Rhizosphere Microbiome β-Diversity but Not EFR1 and CERK1

Plants, 2022
Pathogen associated molecular pattern (PAMP) triggered immunity (PTI) is the first line of plant defense. We hypothesized that the absence of pattern recognition receptors (PRRs) in plants could influence the rhizosphere microbiome.
Jose P. Fonseca, Venkatachalam Lakshmanan, Clarissa Boschiero, Kirankumar S. Mysore   +3 more
doaj   +1 more source

MinION™ Nanopore Sequencing of Skin Microbiome 16S and 16S-23S rRNA Gene Amplicons

Frontiers in Cellular and Infection Microbiology, 2022
Human skin microbiome dysbiosis can have clinical consequences. Characterizing taxonomic composition of bacterial communities associated with skin disorders is important for dermatological advancement in both diagnosis and novel treatments.
Miquel Rozas, Miquel Rozas, François Brillet, Chris Callewaert, Bernhard Paetzold   +4 more
doaj   +1 more source

16S-ITGDB: An Integrated Database for Improving Species Classification of Prokaryotic 16S Ribosomal RNA Sequences

Frontiers in Bioinformatics, 2022
Analyzing 16S ribosomal RNA (rRNA) sequences allows researchers to elucidate the prokaryotic composition of an environment. In recent years, third-generation sequencing technology has provided opportunities for researchers to perform full-length sequence
Yu-Peng Hsieh, Yuan-Mao Hung, Mong-Hsun Tsai, Mong-Hsun Tsai, Liang-Chuan Lai, Liang-Chuan Lai, Eric Y. Chuang, Eric Y. Chuang, Eric Y. Chuang, Eric Y. Chuang   +9 more
doaj   +1 more source