Results 21 to 30 of about 418,926 (311)

Microvariation artifacts introduced by PCR and cloning of closely related 16S rRNA gene sequences [PDF]

, 2001
A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experiment to assess and track sources of artifactual sequence variation in 16S rDNA clone libraries. At least 14% of the recovered clones contained aberrations.
Speksnijder, Arjen, Jong, S., de, Kowalchuk, G.A., Stephen, J.R., De Jong, S., Speksnijder, A.G.C.L., Jong, S. de, Kline, E., Laanbroek, H.J.   +8 more
core   +3 more sources

Species identification and profiling of complex microbial communities using shotgun Illumina sequencing of 16S rRNA amplicon sequences. [PDF]

, 2013
The high throughput and cost-effectiveness afforded by short-read sequencing technologies, in principle, enable researchers to perform 16S rRNA profiling of complex microbial communities at unprecedented depth and resolution. Existing Illumina sequencing
Niranjan Nagarajan, Eliza Xin Pei Ho, Andreas Wilm (7951), Hibberd, Martin Lloyd, Louie Low (400408), Nagarajan, Niranjan, Eliza Xin Pei Ho (400407), Ong, Swee Hoe, Christophe Lay (400406), Martin Lloyd Hibberd (259920), Low, Louie, Kukkillaya, Vinutha Uppoor, Andreas Wilm, Martin Lloyd Hibberd, Lay, Christophe, Niranjan Nagarajan (131213), Wilm, Andreas, Ho, Eliza Xin Pei, Vinutha Uppoor Kukkillaya (400405), Swee Hoe Ong, Louie Low, Swee Hoe Ong (400404), Vinutha Uppoor Kukkillaya, Christophe Lay   +23 more
core   +1 more source

A comparison of rpoB and 16S rRNA as markers in pyrosequencing studies of bacterial diversity [PDF]

, 2012
Background: The 16S rRNA gene is the gold standard in molecular surveys of bacterial and archaeal diversity, but it has the disadvantages that it is often multiple-copy, has little resolution below the species level and cannot be readily interpreted in ...
Kowalchuk, GA, Quince, Christopher, Christopher Quince, de Hollander, M, Quince Christopher, Vos, M., Pijl, Agata S., Vos Michiel, Michiel Vos, Vos, M, Pijl, A.S., Agata S. Pijl (183429), Quince, C., Pijl Agata S., Kowalchuk, G.A., Mattias de Hollander, Hollander, M. de, Michiel Vos (63922), Mattias de Hollander (183437), Vos, Michiel, Quince, C, George A Kowalchuk, Kowalchuk George A., Pijl, AS, de Hollander, Mattias, George A. Kowalchuk (183442), Agata S Pijl, Kowalchuk, George A., Christopher Quince (178494), de Hollander, M., de Hollander Mattias   +30 more
core   +4 more sources

Implementación de una estrategia de espectrometría de doble masa MALDI TOT/TOF para la identificación molecular de bacterias del intestino de trips del banano

Manglar, 2018
Los insectos plaga, conocidos como trips del banano (Chaetanaphothrips signipennis y Frankliniella parvula), vienen causando serios daños económicos al sector bananero de la costa norte del Perú.
Néstor Díaz-Castillo, Dicson Sánchez, Mercedes Oyola, Pedro Masías, R. García-Seminario, Virna Cedeño, Eric Mialhe   +6 more
doaj   +1 more source

High-throughput sequencing of 16S rRNA gene amplicons : effects of extraction procedure, primer length and annealing temperature [PDF]

, 2012
The analysis of 16S-rDNA sequences to assess the bacterial community composition of a sample is a widely used technique that has increased with the advent of high throughput sequencing.
Sergeant Martin J., Penn, Charles W, Cogan Tristan, Pallen, Mark J., Charles W Penn, Chrystala Constantinidou (161352), Charles W. Penn (161371), Sergeant, MJ, Cogan, Tristan, Tristan Cogan (161360), Constantinidou Chrystala, Constantinido, C, Mark J Pallen, Tristan Cogan, Martin J. Sergeant (161344), Constantinidou, Chrystala, Sergeant, Martin J, Penn Charles W., Pallen, Mark J, Sergeant, Martin J., Chrystala Constantinidou, Mark J. Pallen (8904), Penn, CW, Pallen, MJ, Penn, Charles W., Pallen Mark J., Martin J Sergeant, Cogan, TA   +27 more
core   +2 more sources

Boana xerophylla COI-16S

, 2022
Alignment used to estimate divergence time between populations located east and west of the Andes.
Fernando J. M. Rojas-Runjaic (627892), Camilo Montes (2841323), Erika Rodriguez-Muñoz (12048218), Andrew J. Crawford (133496)   +3 more
core   +1 more source

Insight into the molecular diversity of Perreyia flavipes, a comparison across its geographic distribution limits in South America [PDF]

Journal of Insect Biodiversity and Systematics
Perreyia flavipes Konow, 1899 is one of the fairly known species of the Pergidae family. The larval stage of the species presents a high incidence of intoxication outbreaks in South America with an important economic impact on cattle, sheep, and pig ...
Matías Feijoo, Jorge Escalona, Ana Corro, Mauro Pereira Soares, Fernando Dutra   +4 more
doaj   +1 more source

Jumping the green wall: The use of PNA‐DNA clamps to enhance microbiome sampling depth in wildlife microbiome research

Ecology and Evolution, 2020
As microbiome research moves away from model organisms to wildlife, new challenges for microbiome high‐throughput sequencing arise caused by the variety of wildlife diets.
Luis Víquez‐R, Ramona Fleischer, Kerstin Wilhelm, Marco Tschapka, Simone Sommer   +4 more
doaj   +1 more source

Plastid 16S rRNA gene diversity among eukaryotic picophytoplankton sorted by flow cytometry from the South Pacific Ocean [PDF]

, 2011
The genetic diversity of photosynthetic picoeukaryotes was investigated in the South East Pacific Ocean. Genetic libraries of the plastid 16S rRNA gene were constructed on picoeukaryote populations sorted by flow cytometry, using two different primer ...
Cécile Lepère, Vaulot, Daniel, Lepère Cécile, Scanlan, David J, David J. Scanlan, Xiao Li Shi, Shi, Xiao Li, Scanlan, David J., Lepère, Cécile, Scanlan David J., Shi Xiao Li, Vaulot Daniel, David J Scanlan, Daniel Vaulot   +13 more
core   +2 more sources

Intraspecific Genetic Variation of Five Wild Indonesian Striped Snakehead (Channa striata (Bloch, 1793)) Populations Assessed Through 16S rRNA Sequences [PDF]

, 2023
The striped snakehead (Channa striata) belongs to the order Perciformes, suborder Channoidei, and the family Channidae. This fish is native to Sumatra, Java, and Kalimantan, but introduced to Sulawesi and Papua.
Fajar, Ibnu, Hastiwi, Titik Tri, Agiestina, Putri, Suryani, Tuty Ari, Fitriani, Shentarya, Aji, Katon Waskito   +5 more
core   +2 more sources