Results 1 to 10 of about 410,225 (302)

Bacterial genotyping by 16S rRNA mass cataloging [PDF]

BMC Bioinformatics, 2006
Background It has recently been demonstrated that organism identifications can be recovered from mass spectra using various methods including base-specific fragmentation of nucleic acids.
Fox George E, McNichols Roger J, Jackson George W, Willson Richard C   +3 more
doaj   +3 more sources

Molecular cloning of the rRNA genes of Prevotella bryantii B14

Acta Agriculturae Slovenica, 2000
Parts of the rRNA operons of anaerobic rumen bacteria Prevotella bryantii B14 were cloned. 350 colonies carrying pBluescriptII with HindIII DNA fragments were screened with 16S rDNA specific probe and six positive clones were obtained.
Matjaž PETERKA, Gorazd AVGUŠTIN
doaj   +1 more source

Diagnostic Yield and Impact on Antimicrobial Management of 16S rRNA Testing of Clinical Specimens

Microbiology Spectrum, 2022
16S rRNA gene sequencing is increasingly used in clinical practice for bacterial identification of clinical specimens. However, studies on its applicability to direct clinical specimens are limited. Here, we studied the diagnostic yield and impact of 16S
Pruke Eamsakulrat, Pitak Santanirand, Angsana Phuphuakrat   +2 more
doaj   +1 more source

Concatenated 16S rRNA sequence analysis improves bacterial taxonomy [version 3; peer review: 2 approved]

F1000Research, 2023
Background: Microscopic, biochemical, molecular, and computer-based approaches are extensively used to identify and classify bacterial populations.
Bobby Paul
doaj   +1 more source

Microbial signatures in amniotic fluid at preterm birth and association with bronchopulmonary dysplasia

Respiratory Research, 2023
Background Microbiome dysbiosis can have long-lasting effects on our health and induce the development of various diseases. Bronchopulmonary dysplasia (BPD) is a multifactorial disease with pre- and postnatal origins including intra-amniotic infection as
Birte Staude, Silvia Gschwendtner, Tina Frodermann, Frank Oehmke, Thomas Kohl, Susanne Kublik, Michael Schloter, Harald Ehrhardt   +7 more
doaj   +1 more source

In-Depth Analysis of Bacillus anthracis 16S rRNA Genes and Transcripts Reveals Intra- and Intergenomic Diversity and Facilitates Anthrax Detection

mSystems, 2022
Analysis of 16S rRNA (rRNA) genes provides a central means of taxonomic classification of bacterial species. Based on presumed sequence identity among species of the Bacillus cereus sensu lato group, the 16S rRNA genes of B.
Peter Braun, Fee Zimmermann, Mathias C. Walter, Sonja Mantel, Karin Aistleitner, Inga Stürz, Gregor Grass, Kilian Stoecker   +7 more
doaj   +1 more source

Effect of Intragenomic Sequence Heterogeneity among Multiple 16S rRNA Genes on Species Identification of Elizabethkingia

Microbiology Spectrum, 2022
Accurate identification of Elizabethkingia species mostly requires the use of molecular techniques, and 16S rRNA gene sequencing is generally considered the method of choice.
Jiun-Nong Lin, Chung-Hsu Lai, Shang-Yi Lin, Ching-Chi Lee, Nan-Yao Lee, Po-Yu Liu, Chih-Hui Yang, Yi-Han Huang   +7 more
doaj   +1 more source

Information about variations in multiple copies of bacterial 16S rRNA genes may aid in species identification.

PLoS ONE, 2019
Variable region analysis of 16S rRNA gene sequences is the most common tool in bacterial taxonomic studies. Although used for distinguishing bacterial species, its use remains limited due to the presence of variable copy numbers with sequence variation ...
Jerald Conrad Ibal, Huy Quang Pham, Chang Eon Park, Jae-Ho Shin   +3 more
doaj   +1 more source

Dumpster diving for diatom plastid 16S rRNA genes [PDF]

PeerJ, 2021
High throughput sequencing is improving the efficiency of monitoring diatoms, which inhabit and support aquatic ecosystems across the globe. In this study, we explored the potential of a standard V4 515F-806RB primer pair in recovering diatom plastid 16S
Krista L. Bonfantine, Stacey M. Trevathan-Tackett, Ty G. Matthews, Ana Neckovic, Han Ming Gan   +4 more
doaj   +2 more sources

16S rRNA Methylase–producing, Gram-Negative Pathogens, Japan

Emerging Infectious Diseases, 2007
To investigate the exact isolation frequency of 16S rRNA methylase–producing, gram-negative pathogenic bacteria, we tested 87,626 clinical isolates from 169 hospitals. Twenty-six strains from 16 hospitals harbored 16S rRNA methylase genes, which suggests
Kunikazu Yamane, Jun-ichi Wachino, Satowa Suzuki, Naohiro Shibata, Haru Kato, Keigo Shibayama, Kouji Kimura, Kumiko Kai, Satoshi Ishikawa, Yoshiyuki Ozawa, Toshifumi Konda, Yoshichika Arakawa   +11 more
doaj   +1 more source