Efficient Detection of Pathogenic Leptospires Using 16S Ribosomal RNA. [PDF]
Pathogenic Leptospira species cause a prevalent yet neglected zoonotic disease with mild to life-threatening complications in a variety of susceptible animals and humans. Diagnosis of leptospirosis, which primarily relies on antiquated serotyping methods,
Brian T Backstedt +8 more
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Analyzing 16S ribosomal RNA (rRNA) sequences allows researchers to elucidate the prokaryotic composition of an environment. In recent years, third-generation sequencing technology has provided opportunities for researchers to perform full-length sequence
Yuan-Mao Hung +2 more
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Transcriptomic detection of Candidatus Allocryptoplasma (Anaplasmataceae) in Galápagos marine iguanas (Amblyrhynchus cristatus, Iguanidae) [PDF]
Background Globally, the disease ecology of reptiles remains understudied, even for threatened and iconic species such as the Galápagos marine iguana (Amblyrhynchus cristatus).
Flora Uesseler +9 more
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Ribosome assembly is a complex fundamental cellular process that involves assembling multiple ribosomal proteins and several ribosomal RNA species in a highly coordinated yet flexible and resilient manner.
Vignesh M. P. Babu +3 more
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Functional Modification of 16S Ribosomal RNA by Kethoxal [PDF]
Kethoxal reacts with 30S ribosomal subunits to give totally inactive particles, as measured by in vitro protein synthesis. It is postulated that functional modification occurs at the binding site for transfer RNA since ( a ) loss of specific binding of transfer RNA, but not binding of ...
H F, Noller, J B, Chaires
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A comparison of structural and evolutionary attributes of Escherichia coli and Thermus thermophilus small ribosomal subunits: signatures of thermal adaptation. [PDF]
Here we compare the structural and evolutionary attributes of Thermus thermophilus and Escherichia coli small ribosomal subunits (SSU). Our results indicate that with few exceptions, thermophilic 16S ribosomal RNA (16S rRNA) is densely packed compared to
Saurav Mallik, Sudip Kundu
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16S ribosomal RNA gene-based metagenomics: A review
With the advent of contemporary molecular tools, the conventional microbiological isolation, enrichment techniques, and approaches have changed considerably.
Asmita Kamble +2 more
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Selection of reference genes for expression analysis using quantitative real-time PCR in the pea aphid, Acyrthosiphon pisum (Harris) (Hemiptera, Aphidiae). [PDF]
To facilitate gene expression study and obtain accurate qRT-PCR analysis, normalization relative to stable expressed housekeeping genes is required. In this study, expression profiles of 11 candidate reference genes, including actin (Actin), elongation ...
Chunxiao Yang +3 more
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Computer modeling 16 S ribosomal RNA [PDF]
A three-dimensional structure for 16 S RNA has been produced with a computer protocol that is not dependent on human intervention. This protocol improves upon traditional modeling techniques by using distance geometry to fold the molecule in an objective and reproducible fashion.
J M, Hubbard, J E, Hearst
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The structure of a methylated tetraloop in 16S ribosomal RNA [PDF]
Ribosomal RNAs contain many modified nucleotides. The functions of these nucleotides are poorly understood and few of them are strongly conserved. The final stem loop in 16S-like rRNAs is an exception in both regards. In both prokaryotes and eukaryotes, the tetranucleotide loop that caps the 3'-terminal stem contains two N6, N6-dimethyladenosine ...
Rife, Jason P, Moore, Peter B
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