Results 41 to 50 of about 75,285 (265)

Association between semen microbiome disorder and sperm DNA damage

Microbiology Spectrum
DNA fragmentation index (DFI), a new biomarker to diagnose male infertility, is closely associated with poor reproductive outcomes. Previous research reported that seminal microbiome correlated with sperm DNA integrity, suggesting that the microbiome may
Junxian He, Menghui Ma, Zhenhan Xu, Jintao Guo, Haicheng Chen, Xing Yang, Peigen Chen, Guihua Liu   +7 more
doaj   +1 more source

Influence of 16S Ribosomal RNA Gene Polymerase Chain Reaction and Sequencing on Antibiotic Management of Bone and Joint Infections

Canadian Journal of Infectious Diseases and Medical Microbiology, 2013
INTRODUCTION: Amplification of the 16S ribosomal RNA gene by polymerase chain reaction (PCR) followed by analysis of generated sequences can be an important adjunct to conventional cultures.
B Alraddadi, S Al-Azri, KR Forward
doaj   +1 more source

Deciphering transcriptional plasticity in pancreatic ductal adenocarcinoma reveals alterations in sensory neuron innervation

Molecular Oncology, EarlyView.
Pancreatic sensory neurons innervating healthy and PDAC tissue were retrogradely labeled and profiled by single‐cell RNA sequencing. Tumor‐associated innervation showed a dominant neurofilament‐positive subtype, altered mitochondrial gene signatures, and reduced non‐peptidergic neurons.
Elena Genova, Michele Montrone, Uday Rangaswamy, Francesco Diversi, Irene Schiavo, Denise Ferrarini, Roberta Di Florio, Irene Longo, Michele Coscia, Nicola Zamboni, Giorgia Demontis, Lisa Veghini, Vincenzo Corbo, Remo Sanges, Paul Heppenstall   +14 more
wiley   +1 more source

16S Ribosomal RNA-Based Analysis of Thermophilic Bacteria in Gedongsongo Hot Spring

Microbiology Indonesia, 2010
Denaturing gradient gel electrophoresis was used to identify the bacterial community at the Gedongsongo (WGS-2) hot spring. The bacterial samples were obtained from both culture dependent and independent strategies.
AGUSTINA LULUSTYANINGATI NURUL AMININ1, FIDA MADAYANTI, PINGKAN ADITIAWATI, . AKHMALOKA   +3 more
doaj   +1 more source

Heterozygous loss‐of‐function alleles associate the conserved 3′‐5′ exoribonuclease EXOSC10 with hypersensitivity to the anticancer drug 5‐fluorouracil

Molecular Oncology, EarlyView.
EXOSC10, an essential nuclear RNA exosome‐associated 3′‐5′ exoribonuclease, is inhibited by the anticancer drug 5‐fluorouracil (5‐FU), and EXOSC10 depletion increases 5‐FU sensitivity. The colon‐cancer variant EXOSC10S402T, located in a proteolysis motif, is stable and nuclear but nonfunctional in vivo.
Radhika Sain, Yann Le Page, Frédéric Percevault, Emmanuelle Becker, Luc Negroni, Almudena Fernandez, Marta Cantero, Diego Muñoz‐Santos, Lluís Montoliu, Cyrille Garnier, Michael Primig   +10 more
wiley   +1 more source

The complete mitochondrial genome of Glossaulax reiniana (Littorinimorpha: Naticidae)

Mitochondrial DNA. Part B. Resources, 2018
In the present study, the complete mitochondrial genome of Glossaulax reiniana was determined using the next-generation sequencing. The circular genome was found to be 15,254 bp in length and had an overall nucleotide composition of 30.6% A, 14.1% C, 15 ...
Peng-Yu Li, Yi Yang, Yong-Guo Li, Shao-E Sun   +3 more
doaj   +1 more source

Frequency of Bacetrial Content Finding in Persistant Periapical Lesions

Acta Stomatologica Croatica, 2017
Objectives: To determine the percentage of persistant apical lesions positive for bacterial nucleic acids, to detect microorganisms difficult to cultivate in persistant apical lesions by PCR and relate them to endodontic failure, clinical symptoms and ...
Joško Grgurević, Ana Ivanišević Malčić, Arjana Tambić Andrašević, Goranka Prpić Mehičić, Sania Kuzmac, Silvana Jukić   +5 more
doaj   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

MOLECULAR IDENTIFICATION OF SEVERAL CLINICALLYIMPORTANT BACTERIA INCLUDING ENTEROBACTERIA BYPARTIAL 16S RIBOSOMAL RNA GENE SEQUENCE COMPARISON

Zdravniški Vestnik, 2008
BACKGROUND The correct identification of bacterial isolates is paramount for the correct diagnosis andtreatment of patients. The standard approach for bacterial identification relies on phenotypic tests, which are often time-consuming and unreliable when
Janez Šimenc, Andrej Golle, Uroš Potočnik   +2 more
doaj  

Acute caffeine treatment protects the developing retina from ischemia‐induced cell death

FEBS Open Bio, EarlyView.
Caffeine reduces cell death in the developing retina under ischemia (OGD). This effect does not involve BDNF upregulation or antioxidant pathways (NRF2/VEGF). Neuroprotection occurs mainly through adenosine A2A receptor antagonism, decreasing glutamate release and excitotoxicity, highlighting caffeine's potential as an acute neuroprotective agent in ...
Amanda Alves Nascimento, Danniel Pereira‐Figueiredo, Gabriella Grossi de Lima Dias, Gabriel Ferreira dos Santos, Mariana Rodrigues Pereira, Rafael Brito, Karin C Calaza   +6 more
wiley   +1 more source