Results 41 to 50 of about 1,272,206 (308)
BMC Genomics, 2023 Background Single-cell RNA-sequencing (scRNA-seq) relies on PCR amplification to retrieve information from vanishingly small amounts of starting material. To selectively enrich mRNA from abundant non-polyadenylated transcripts, poly(A) selection is a key
Kuang-Tse Wang, Carolyn E. Adler
doaj +1 more source
Optimizing PCR primers targeting the bacterial 16S ribosomal RNA gene
BMC Bioinformatics, 2018 Background Targeted amplicon sequencing of the 16S ribosomal RNA gene is one of the key tools for studying microbial diversity. The accuracy of this approach strongly depends on the choice of primer pairs and, in particular, on the balance between ...
Francesco Sambo +9 more
doaj +1 more source
Hayati Journal of Biosciences, 2015 A community of thermophiles within Tanjung Sakti Hot Spring (South Sumatera) have been cultivated and identified based on 16S ribosomal RNA gene sequence. The hot spring has temperature 80 °C–91 °C and pH 7–8.
Heni Yohandini, Julinar, Muharni
doaj +1 more source
Role of 16‐S RNA in Ribosome Messenger Recognition [PDF]
European Journal of Biochemistry, 1980 The deoxyoctanucleotide (5′‐3′)d(A‐A‐G‐G‐A‐G‐G‐T), which is complementary to the 3′ end of 16‐S RNA, inhibits the formation of the complex between the 30‐S subunit and MS2 RNA described in the preceding paper. If the complex is preformed, the octanucleotide cannot prevent entry of the complex into the ribosome cycle upon supplementation with the ...
C, Backendorf +5 more
openaire +2 more sources
PLoS ONE, 2019 The ribosome small subunit is expressed in all living cells. It performs numerous essential functions during translation, including formation of the initiation complex and proofreading of base-pairs between mRNA codons and tRNA anticodons.
Andrew M Smith +4 more
doaj +1 more source
Chromosomal 16S Ribosomal RNA Methyltransferase RmtE1 in Escherichia coli Sequence Type 448
Emerging Infectious Diseases, 2017 We identified rmtE1, an uncommon 16S ribosomal methyltransferase gene, in an aminoglycoside- and cephalosporin-resistant Escherichia coli sequence type 448 clinical strain co-harboring blaCMY-2.
Bin Li, Marissa P. Pacey, Yohei Doi
doaj +1 more source
Taxonomic and Characterization Methods of Streptomyces: A Review
Progress in Microbes and Molecular Biology, 2018 The 16S ribosomal RNA gene is the gold standard for taxonomic identification and phylogenetic study of most of the bacteria. However, the resolution of 16S rRNA gene is found to be insufficient to distinguish closely related Streptomyces species within ...
Jodi Woan-Fei Law +4 more
doaj +1 more source
Bakteri ribosoomide uurimus keemilise modifitseerimise meetoditega [PDF]
, 2010 Väitekirja elektrooniline versioon ei sisalda publikatsioone.Ribosoom on suur makromolekulaarne kompleks, mis kodeerib päriliku informatsiooni valgulisse olemusse.
Pulk, Arto
core
Translophagy—A potential link between autophagy impairment and translational errors
FEBS Letters, EarlyView.Neurodegenerative diseases are characterised by the accumulation of abnormal proteins and protein aggregates, but their origin often remains unknown. We propose that selective autophagy removes damaged protein‐making machinery, preventing errors during protein synthesis.
Mykola V. Korolchuk +11 more
wiley +1 more source
Charakterisierung eines Protein:RNA-Komplexes : die Funktion von ribosomal binding factor A [PDF]
, 2007 In this thesis the three dimensional solution strucutre of the RbfA protein from Thermotoga maritima was solved using multidimensional heteronuclear NMR spectroscopy. The RbfA protein binds to the helix I region of the 16S rRNA. To gain insights into the
Grimm, Steffen Kaspar
core