Results 11 to 20 of about 1,272,206 (308)

Ribosomal RNA methylation by GidB modulates discrimination of mischarged tRNA. [PDF]

Elife
Despite redundant cellular pathways to minimize translational errors, errors in protein synthesis are common. Pathways and mechanisms to minimize errors are classified as pre-ribosomal or ribosomal. Pre-ribosomal pathways are primarily concerned with the
Bi Z, Chen YX, Young ID, Dandan MT, Joshi H, Su HW, Chen Y, Hong J, Fraser JS, Javid B.   +9 more
europepmc   +2 more sources

16S ribosomal RNA modification drives transcript-specific translation efficiency. [PDF]

Cell Rep
Summary: Bacterial ribosomal RNAs (rRNAs) are decorated with conserved nucleotide modifications, but the functionality of these modifications is often underexplored. MraW (RsmH) is a 16S rRNA methyltransferase.
Park ZM, Savage CR, Decker-Farrell AR, Tai CH, Maity TK, Yang W, Jenkins LM, Ramamurthi KS.   +7 more
europepmc   +2 more sources

Efficient Detection of Pathogenic Leptospires Using 16S Ribosomal RNA. [PDF]

PLoS ONE, 2015
Pathogenic Leptospira species cause a prevalent yet neglected zoonotic disease with mild to life-threatening complications in a variety of susceptible animals and humans. Diagnosis of leptospirosis, which primarily relies on antiquated serotyping methods,
Brian T Backstedt, Ozlem Buyuktanir, Janet Lindow, Elsio A Wunder, Mitermayer G Reis, Sahar Usmani-Brown, Michel Ledizet, Albert Ko, Utpal Pal   +8 more
doaj   +5 more sources

Aptazyme-Mediated Regulation of 16S Ribosomal RNA

Chemistry & Biology, 2010
Developing artificial genetic switches in order to control gene expression via an external stimulus is an important aim in chemical and synthetic biology. Here, we expand the application range of RNA switches to the regulation of 16S rRNA function in Escherichia coli.
Wieland, Markus, Hartig, Jörg S., Berschneider, Barbara, Erlacher, Matthias D.   +3 more
openaire   +4 more sources

Novel and Efficient Method for Diagnosing Infective Endocarditis Using 16S Ribosomal RNA Gene Amplicon Sequence

JACC: Case Reports, 2023
We present 2 cases that both developed infective endocarditis and underwent mitral valve replacement. In addition to positive blood culture and echocardiographic findings, such as vegetation or mitral valve perforation, the 16S ribosomal RNA gene ...
Akihisa Kataoka, MD, PhD, Takahiko Shiba, DDS, PhD, Sayaka Katagiri, DDS, PhD, Shuhei Koyama, MD, Kento Kito, MD, Tatsuya Ota, MD, Taiga Katayama, MD, PhD, Yujin Ohsugi, DDS, PhD, Takahiko Nagai, DDS, Ryota Kobayashi, DDS, Keiji Komatsu, DDS, PhD, Masahiro Urata, MD, Tomohiro Imazuru, MD, PhD, Takanori Iwata, DDS, PhD, Ken Kozuma, MD, PhD   +14 more
doaj   +2 more sources

Influence of 16S Ribosomal RNA Gene Polymerase Chain Reaction and Sequencing on Antibiotic Management of Bone and Joint Infections [PDF]

Canadian Journal of Infectious Diseases and Medical Microbiology, 2013
INTRODUCTION: Amplification of the 16S ribosomal RNA gene by polymerase chain reaction (PCR) followed by analysis of generated sequences can be an important adjunct to conventional cultures.
B Alraddadi, S Al-Azri, KR Forward
doaj   +2 more sources

Updating the 97% identity threshold for 16S ribosomal RNA OTUs [PDF]

Bioinformatics, 2017
Abstract The 16S ribosomal RNA (rRNA) gene is widely used to survey microbial communities. Sequences are often clustered into Operational Taxonomic Units (OTUs) as proxies for species. The canonical clustering threshold is 97% identity, which was proposed in 1994 when few 16S rRNA sequences were available, motivating a reassessment on ...
Robert C. Edgar
openaire   +3 more sources

Functional Modification of 16S Ribosomal RNA by Kethoxal [PDF]

Proceedings of the National Academy of Sciences, 1972
Kethoxal reacts with 30S ribosomal subunits to give totally inactive particles, as measured by in vitro protein synthesis. It is postulated that functional modification occurs at the binding site for transfer RNA since ( a ) loss of specific binding of ...
H F, Noller, J B, Chaires
openaire   +2 more sources

A comparison of structural and evolutionary attributes of Escherichia coli and Thermus thermophilus small ribosomal subunits: signatures of thermal adaptation. [PDF]

PLoS ONE, 2013
Here we compare the structural and evolutionary attributes of Thermus thermophilus and Escherichia coli small ribosomal subunits (SSU). Our results indicate that with few exceptions, thermophilic 16S ribosomal RNA (16S rRNA) is densely packed compared to
Saurav Mallik, Sudip Kundu
doaj   +1 more source

16S ribosomal RNA gene-based metagenomics: A review

Biomedical Research Journal, 2020
With the advent of contemporary molecular tools, the conventional microbiological isolation, enrichment techniques, and approaches have changed considerably.
Asmita Kamble, Shriya Sawant, Harinder Singh   +2 more
doaj   +1 more source