Intraspecific Genetic Variation of Five Wild Indonesian Striped Snakehead (Channa striata (Bloch, 1793)) Populations Assessed Through 16S rRNA Sequences [PDF]
The striped snakehead (Channa striata) belongs to the order Perciformes, suborder Channoidei, and the family Channidae. This fish is native to Sumatra, Java, and Kalimantan, but introduced to Sulawesi and Papua.
Fajar, Ibnu +5 more
core +2 more sources
The potential for aerobic and anaerobic microbial natural attenuation of PCBs in freshwater sediments is described by PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets generated, in duplicate, from 27 sediment samples ...
Timothy E. Mattes +6 more
doaj +1 more source
A renaissance for the pioneering 16S rRNA gene [PDF]
Culture-independent molecular surveys using the 16S rRNA gene have become a mainstay for characterizing microbial community structure over the past quarter century. More recently this approach has been overshadowed by metagenomics, which provides a global overview of a community's functional potential rather than just an inventory of its inhabitants ...
Tringe, SG, Hugenholtz, P
openaire +5 more sources
16S rRNA sequencing analysis: the devil is in the details [PDF]
User-friendly computational tools for 16S ribosomal RNA (rRNA) sequencing analysis enable researchers who are not bioinformaticians to analyze and interpret sequencing data from microbial communities. These tools' easy-to-use interfaces belie the sophisticated and rapidly-evolving science of their underlying algorithms.
Amy M. Tsou +3 more
openaire +3 more sources
A highly resolved taxonomy for ammonia-oxidizing archaea (AOA) based on the alpha subunit of ammonia monooxygenase (amoA) was recently established, which uncovered novel environmental patterns of AOA, challenging previous generalizations.
Haitao Wang +6 more
doaj +1 more source
Species identification and profiling of complex microbial communities using shotgun Illumina sequencing of 16S rRNA amplicon sequences. [PDF]
The high throughput and cost-effectiveness afforded by short-read sequencing technologies, in principle, enable researchers to perform 16S rRNA profiling of complex microbial communities at unprecedented depth and resolution. Existing Illumina sequencing
Niranjan Nagarajan +23 more
core +1 more source
A comparison of rpoB and 16S rRNA as markers in pyrosequencing studies of bacterial diversity [PDF]
Background: The 16S rRNA gene is the gold standard in molecular surveys of bacterial and archaeal diversity, but it has the disadvantages that it is often multiple-copy, has little resolution below the species level and cannot be readily interpreted in ...
Kowalchuk, GA +30 more
core +4 more sources
Plastid 16S rRNA gene diversity among eukaryotic picophytoplankton sorted by flow cytometry from the South Pacific Ocean [PDF]
The genetic diversity of photosynthetic picoeukaryotes was investigated in the South East Pacific Ocean. Genetic libraries of the plastid 16S rRNA gene were constructed on picoeukaryote populations sorted by flow cytometry, using two different primer ...
Cécile Lepère +13 more
core +2 more sources
RmtC 16S rRNA Methyltransferase in Australia [PDF]
Proteus mirabilis isolate JIE273 was recovered in March 2007 from the urine of an inpatient at Blacktown Hospital, Sydney. In vitro susceptibility tests (Phoenix NMIC/ID-101 panel; BD, Franklin Lakes, NJ) indicated that JIE273 was susceptible to imipenem (MIC, ≤2 μg/ml), cefoxitin (MIC, ≤4 μg/
Zhiyong, Zong +2 more
openaire +2 more sources
Microvariation artifacts introduced by PCR and cloning of closely related 16S rRNA gene sequences [PDF]
A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experiment to assess and track sources of artifactual sequence variation in 16S rDNA clone libraries. At least 14% of the recovered clones contained aberrations.
Speksnijder, Arjen +8 more
core +3 more sources

