Results 21 to 30 of about 399,449 (201)

Latitudinal variation in the potential activity of Atlantic Ocean bacterioplankton revealed through 16S rRNA and 16S rRNA gene metabarcoding

open access: yesFrontiers in Marine Science, 2023
The activities of bacterioplankton sustain open ocean biogeochemical and ecological processes, however, little is known about the activity of specific bacterioplankton, especially related to their biogeography across oceanic scales.
Ro Allen   +5 more
doaj   +1 more source

Engineering CRISPR/Cas9 to mitigate abundant host contamination for 16S rRNA gene-based amplicon sequencing

open access: yesMicrobiome, 2020
Background High-throughput sequencing of bacterial 16S rRNA gene (16S-seq) is a useful and common method for studying bacterial community structures. However, contamination of the 16S rRNA genes from the mitochondrion and plastid hinders the sensitive ...
Luyang Song, Kabin Xie
doaj   +1 more source

A renaissance for the pioneering 16S rRNA gene [PDF]

open access: yesCurrent Opinion in Microbiology, 2008
Culture-independent molecular surveys using the 16S rRNA gene have become a mainstay for characterizing microbial community structure over the past quarter century. More recently this approach has been overshadowed by metagenomics, which provides a global overview of a community's functional potential rather than just an inventory of its inhabitants ...
Tringe, SG, Hugenholtz, P
openaire   +4 more sources

A Quantitative Sequencing Framework for Absolute Abundance Measurements of Mucosal and Lumenal Microbial Communities [PDF]

open access: yes, 2020
A fundamental goal in microbiome studies is determining which microbes affect host physiology. Standard methods for determining changes in microbial taxa measure relative, rather than absolute abundances.
Barlow, Jacob T.   +2 more
core   +1 more source

Microbial communities in polychlorinated biphenyl (PCB)-contaminated wastewater lagoon sediments: PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets

open access: yesData in Brief, 2021
The potential for aerobic and anaerobic microbial natural attenuation of PCBs in freshwater sediments is described by PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets generated, in duplicate, from 27 sediment samples ...
Timothy E. Mattes   +6 more
doaj   +1 more source

RmtC 16S rRNA Methyltransferase in Australia [PDF]

open access: yesAntimicrobial Agents and Chemotherapy, 2008
Proteus mirabilis isolate JIE273 was recovered in March 2007 from the urine of an inpatient at Blacktown Hospital, Sydney. In vitro susceptibility tests (Phoenix NMIC/ID-101 panel; BD, Franklin Lakes, NJ) indicated that JIE273 was susceptible to imipenem (MIC, ≤2 μg/ml), cefoxitin (MIC, ≤4 μg/
Zhiyong, Zong   +2 more
openaire   +2 more sources

16S rRNA sequencing analysis: the devil is in the details [PDF]

open access: yesGut Microbes, 2020
User-friendly computational tools for 16S ribosomal RNA (rRNA) sequencing analysis enable researchers who are not bioinformaticians to analyze and interpret sequencing data from microbial communities. These tools' easy-to-use interfaces belie the sophisticated and rapidly-evolving science of their underlying algorithms.
Amy M. Tsou   +3 more
openaire   +3 more sources

Mycoplasma gallisepticum16S rRNA genes [PDF]

open access: yesFEMS Microbiology Letters, 1995
The genome of Mycoplasma gallisepticum A5969 contains a truncated pseudogene for 16S rRNA in addition to a single unsplit rRNA-operon and a second discontinuous set of rRNA genes. Other M. gallisepticum strains tested do not possess the truncated gene.
A, Skamrov   +3 more
openaire   +3 more sources

Linking 16S rRNA Gene Classification to amoA Gene Taxonomy Reveals Environmental Distribution of Ammonia-Oxidizing Archaeal Clades in Peatland Soils

open access: yesmSystems, 2021
A highly resolved taxonomy for ammonia-oxidizing archaea (AOA) based on the alpha subunit of ammonia monooxygenase (amoA) was recently established, which uncovered novel environmental patterns of AOA, challenging previous generalizations.
Haitao Wang   +6 more
doaj   +1 more source

Molecular cloning of the rRNA genes of Prevotella bryantii B14

open access: yesActa Agriculturae Slovenica, 2000
Parts of the rRNA operons of anaerobic rumen bacteria Prevotella bryantii B14 were cloned. 350 colonies carrying pBluescriptII with HindIII DNA fragments were screened with 16S rDNA specific probe and six positive clones were obtained.
Matjaž PETERKA, Gorazd AVGUŠTIN
doaj   +1 more source

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