Results 11 to 20 of about 399,449 (201)
Circularization of 23S rRNA but not 16S rRNA within archaeal ribosomes [PDF]
Genome BiologyBackground Processing of archaeal 16S and 23S rRNAs is believed to involve excision of individual rRNAs from polycistronic precursors, circularization of excised rRNAs, and re-linearization before the incorporation into ribosomes.
Ling-Dong Shi +6 more
doaj +5 more sources
A comparison of 16S rRNA-gene and 16S rRNA-transcript derived microbial communities in bulk and rhizosphere soils [PDF]
Frontiers in MicrobiologyRoot exudates in a plant’s rhizosphere alters microbial community membership and activity, which can in turn alter a plant’s health and fitness. In this study we characterized bacterial community composition, using 16S rRNA-gene (DNA) sequencing to ...
Alessandra Ceretto +4 more
doaj +4 more sources
Simultaneous measurement of 16S-rRNA and pre-16S-rRNA as a strategy to monitor clinical tuberculosis [PDF]
Frontiers in AntibioticsBackgroundCulture-based biomarkers of TB treatment response monitoring, e.g., Mycobacterial Growth Indicatory Tube (MGIT), are compromised when bacteria enter a non-replicating persister phase limiting the measurement of antibiotic efficacy and ...
Evelin Dombay +8 more
doaj +2 more sources
Utility of 16S rRNA PCR performed on clinical specimens in patient management
International Journal of Infectious Diseases, 2017 Background: Broad-range 16S rRNA PCR can be used for the detection and identification of bacteria from clinical specimens in patients for whom there is a high suspicion of infection and cultures are negative.
A. Akram +4 more
exaly +3 more sources
Bacterial genotyping by 16S rRNA mass cataloging [PDF]
BMC Bioinformatics, 2006 Background It has recently been demonstrated that organism identifications can be recovered from mass spectra using various methods including base-specific fragmentation of nucleic acids.
Fox George E +3 more
doaj +3 more sources
Frontiers in Microbiology, 2023 The Lactobacillaceae are lactic acid bacteria harnessed to deliver important outcomes across numerous industries, and their unambiguous, species-level identification from mixed community environments is an important endeavor.
Sandra A. Olivier +9 more
doaj +1 more source
PCR biases distort bacterial and archaeal community structure in pyrosequencing datasets [PDF]
, 2012 As 16S rRNA gene targeted massively parallel sequencing has become a common tool for microbial diversity investigations, numerous advances have been made to minimize the influence of sequencing and chimeric PCR artifacts through rigorous quality control ...
Ameet J. Pinto +2 more
core +10 more sources
Phylogenetic Clustering of Soil Microbial Communities by 16S rRNA but Not 16S rRNA Genes [PDF]
Applied and Environmental Microbiology, 2012 ABSTRACT We evaluated phylogenetic clustering of bacterial and archaeal communities from redox-dynamic subtropical forest soils that were defined by 16S rRNA and rRNA gene sequences. We observed significant clustering for the RNA-based communities but not the DNA-based communities, as well as increasing clustering over time of the highly ...
Kristen M, DeAngelis, Mary K, Firestone
openaire +2 more sources
Variable Region Sequences Influence 16S rRNA Performance
Microbiology Spectrum, 2023 16S rRNA gene sequences are commonly analyzed for taxonomic and phylogenetic studies because they contain variable regions that can help distinguish different genera. However, intra-genus distinction using variable region homology is often impossible due
Nikhil Bose, Sean D. Moore
doaj +1 more source
OTU analysis using metagenomic shotgun sequencing data. [PDF]
PLoS ONE, 2012 Because of technological limitations, the primer and amplification biases in targeted sequencing of 16S rRNA genes have veiled the true microbial diversity underlying environmental samples. However, the protocol of metagenomic shotgun sequencing provides
Xiaolin Hao, Ting Chen
doaj +1 more source