Reconstructing 16S rRNA genes in metagenomic data [PDF]
Abstract Metagenomic data, which contains sequenced DNA reads of uncultured microbial species from environmental samples, provide a unique opportunity to thoroughly analyze microbial species that have never been identified before. Reconstructing 16S ribosomal RNA, a phylogenetic marker gene, is usually required to analyze the composition
Cheng Yuan 0001 +3 more
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Comparative analysis of pyrosequencing and a phylogenetic microarray for exploring microbial community structures in the human distal intestine [PDF]
BACKGROUND: Variations in the composition of the human intestinal microbiota are linked to diverse health conditions. High-throughput molecular technologies have recently elucidated microbial community structure at much higher resolution than was ...
Vos, W.M., de +47 more
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Correlation between Protection against Sepsis by Probiotic Therapy and Stimulation of a Novel Bacterial Phylotype [PDF]
Item does not contain fulltextProphylactic probiotic therapy has shown beneficial effects in an experimental rat model for acute pancreatitis on the health status of the animals.
Smidt, H. +24 more
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16S rRNA Phylogeny of Sponge-Associated Cyanobacteria [PDF]
ABSTRACT Phylogenetic analyses of 16S rRNA sequences of sponge-associated cyanobacteria showed them to be polyphyletic, implying that they derived from multiple independent symbiotic events. Most of the symbiont sequences were affiliated to a group of Synechococcus and Prochlorococcus
Laura, Steindler +3 more
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Mitochondrial 16S rRNA Sequence Diversity of Hominoids [PDF]
We determined nucleotide sequences of the 16S rRNA gene of mitochondrial DNA (mtDNA) (about 1.6 kb) for 35 chimpanzee, 13 bonobo, 10 gorilla, 16 orangutan, and 23 gibbon individuals. We compared those data with published sequences and estimated nucleotide diversity for each species. All the ape species showed higher diversity than human.
R, Noda +8 more
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Groundtruthing next-gen sequencing for microbial ecology-biases and errors in community structure estimates from PCR amplicon pyrosequencing [PDF]
Analysis of microbial communities by high-throughput pyrosequencing of SSU rRNA gene PCR amplicons has transformed microbial ecology research and led to the observation that many communities contain a diverse assortment of rare taxa-a phenomenon termed ...
Polson, Shawn W. +20 more
core +1 more source
Clinical usefulness of 16S ribosomal RNA real-time PCR for the diagnosis of scrub typhus
Scrub typhus is a major acute febrile disease in the Asia–Pacific region. The purpose of the present study is to investigate the clinical usefulness of real-time PCR (Q-PCR) of 16S rRNA for the diagnosis of scrub typhus.
Na Ra Yun +4 more
doaj +1 more source
Yeast Rrp8p, a novel methyltransferase responsible for m1A 645 base modification of 25S rRNA [PDF]
Ribosomal RNA undergoes various modifications to optimize ribosomal structure and expand the topological potential of RNA. The most common nucleotide modifications in ribosomal RNA (rRNA) are pseudouridylations and 2'-O methylations (Nm), performed by H ...
Sharma, Sunny +5 more
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The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates [PDF]
Using three reference strains of Brachyspira hyodysenteriae (B204, B234, B169), one B. pilosicoli (P43/6/78), one B. murdochii (56-150), one B. intermedia (PWS/A), one B. innocens (B256) and ten Korean isolates, PCR-RFLP analysis of DNA encoding 23S rRNA
Lee, J.L. +3 more
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Variable copy number, intra-genomic heterogeneities and lateral transfers of the 16S rRNA gene in Pseudomonas. [PDF]
Even though the 16S rRNA gene is the most commonly used taxonomic marker in microbial ecology, its poor resolution is still not fully understood at the intra-genus level.
Josselin Bodilis +3 more
doaj +1 more source

