Results 41 to 50 of about 292,385 (304)

Microvariation artifacts introduced by PCR and cloning of closely related 16S rRNA gene sequences

, 2001
A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experiment to assess and track sources of artifactual sequence variation in 16S rDNA clone libraries. At least 14% of the recovered clones contained aberrations.
Speksnijder, Arjen, Jong, S., de, Kowalchuk, G.A., Stephen, J.R., De Jong, S., Speksnijder, A.G.C.L., Jong, S. de, Kline, E., Laanbroek, H.J.   +8 more
core   +1 more source

Comparative evaluation of sequence analysis of 16S rRNA and rpoB genes for identification of aquatic mycobacteria [PDF]

, 2016
The nucleotide sequences of partial 16S rRNA and bacterial RNA polymerase ß-subunit (rpoB) genes for 57 mycobacterial strains were determined.
Pourahmad, Fazel, Richards, Randolph
core   +1 more source

Characterization of spiny lobsters from Bangladesh waters using morphology, COI and 16S rRNA sequences

Heliyon, 2022
This study aims to taxonomically identify and characterise the phylogenetic relationships of spiny lobsters based on mitochondrial cytochrome c oxidase I (COI) and 16S rRNA genes from Bangladesh waters.
Md. Sagir Ahmed, Anindita Barua, Sujan Kumar Datta, Tonmoy Saha, Durjoy Raha Antu, Sumaiya Ahmed   +5 more
doaj   +1 more source

Mitochondrial 16S rRNA Sequence Diversity of Hominoids [PDF]

Journal of Heredity, 2001
We determined nucleotide sequences of the 16S rRNA gene of mitochondrial DNA (mtDNA) (about 1.6 kb) for 35 chimpanzee, 13 bonobo, 10 gorilla, 16 orangutan, and 23 gibbon individuals. We compared those data with published sequences and estimated nucleotide diversity for each species. All the ape species showed higher diversity than human.
R, Noda, C G, Kim, O, Takenaka, R E, Ferrell, T, Tanoue, I, Hayasaka, S, Ueda, T, Ishida, N, Saitou   +8 more
openaire   +2 more sources

Analysis of the bacterial communities in the rhizosphere of Chrysanthemum via denaturing gradient gel electrophoresis of PCR- amplified 16S rRNA as well as DNA fragments coding for 16S rRNA

, 2001
The effect of developing chrysanthemum roots on the presence and activity of bacterial populations in the rhizosphere was examined by using culture-independent methods.
Anneke Keijzer, Van Elsas, J.D., Duineveld, B.M., Kowalchuk, G.A., Van Veen, J.A., George A. Kowalchuk, Jan Dirk van Elsas, Keijzer, A., Veen, J.A., van, Bernadette M. Duineveld, Elsas, J.D., van, Johannes A. van Veen   +11 more
core   +1 more source

Louse (Insecta : Phthiraptera) mitochondrial 12S rRNA secondary structure is highly variable [PDF]

, 2002
Lice are ectoparasitic insects hosted by birds and mammals. Mitochondrial 12S rRNA sequences obtained from lice show considerable length variation and are very difficult to align.
Page, R.D.M., Cruickshank, R., Johnson, K.P.   +2 more
core   +1 more source

16S-ITGDB: An Integrated Database for Improving Species Classification of Prokaryotic 16S Ribosomal RNA Sequences

Frontiers in Bioinformatics, 2022
Analyzing 16S ribosomal RNA (rRNA) sequences allows researchers to elucidate the prokaryotic composition of an environment. In recent years, third-generation sequencing technology has provided opportunities for researchers to perform full-length sequence
Yu-Peng Hsieh, Yuan-Mao Hung, Mong-Hsun Tsai, Mong-Hsun Tsai, Liang-Chuan Lai, Liang-Chuan Lai, Eric Y. Chuang, Eric Y. Chuang, Eric Y. Chuang, Eric Y. Chuang   +9 more
doaj   +1 more source

The human gut microbiome across the life course

FEBS Letters, EarlyView.
Despite significant individual variation and continuous change throughout life, the human gut microbiome follows some life stage‐specific trends. This article provides a brief overview of how gut microbiome composition shifts across different phases of life. Created in BioRender. Özkurt, E. (2026) https://BioRender.com/8q4nrnc.
Alise J. Ponsero, Basak Bahcivanci, Antonietta Hayhoe, Animesh Acharjee, Ezgi Özkurt   +4 more
wiley   +1 more source

Choice of 16S Ribosomal RNA Primers Impacts Male Urinary Microbiota Profiling

Frontiers in Cellular and Infection Microbiology, 2022
Accessibility to next-generation sequencing (NGS) technologies has enabled the profiling of microbial communities living in distinct habitats. 16S ribosomal RNA (rRNA) gene sequencing is widely used for microbiota profiling with NGS technologies.
Vitor Heidrich, Vitor Heidrich, Lilian T. Inoue, Paula F. Asprino, Fabiana Bettoni, Antonio C. H. Mariotti, Diogo A. Bastos, Denis L. F. Jardim, Marco A. Arap, Anamaria A. Camargo   +9 more
doaj   +1 more source

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

FEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source