Results 1 to 10 of about 183,216 (261)

Variable copy number, intra-genomic heterogeneities and lateral transfers of the 16S rRNA gene in Pseudomonas. [PDF]

PLoS ONE, 2012
Even though the 16S rRNA gene is the most commonly used taxonomic marker in microbial ecology, its poor resolution is still not fully understood at the intra-genus level.
Josselin Bodilis, Sandrine Nsigue-Meilo, Ludovic Besaury, Laurent Quillet   +3 more
doaj   +1 more source

Community analysis of picocyanobacteria in an oligotrophic lake by cloning 16S rRNA gene and 16S rRNA gene amplicon sequencing

The Journal of General and Applied Microbiology, 2015
In this study, the picocyanobacterial species composition of Lake Miyagase was examined by analyzing the 16S rRNA gene in a clone library and by amplicon sequencing using a benchtop next-generation sequencer. Five separate samples were analyzed from different days over a ten-month period. In the picocyanobacterial lineage, 9 and 12 OTUs were identified
Fujimoto, Naoshi, Mizuno, Keigo, Yokoyama, Tomoki, Ohnishi, Akihiro, Suzuki, Masaharu, Watanabe, Satoru, Komatsu, Kenji, Sakata, Yoichi, Kishida, Naohiro, Akiba, Michihiro, Matsukura, Satoko   +10 more
openaire   +3 more sources

Two different 16S rRNA genes in a mycobacterial strain [PDF]

Journal of Clinical Microbiology, 1996
Sequencing of the gene coding for 16S rRNA (16S rDNA) is a well-established method used to identify bacteria, particularly mycobacteria. Unique sequences allow identification of a particular genus and species. If more than one 16S rDNA is present on one mycobacterial genome, their sequences are assumed to be strictly or almost identical.
Bescher Ninet, Béatrice Alice, Monod, M., Emler, Stefan, Pawlowski, Jan Wojciech, Metral, Claudine, Rohner, Peter, Auckenthaler, Raymond, Hirschel, Bernard   +7 more
openaire   +4 more sources

Information about variations in multiple copies of bacterial 16S rRNA genes may aid in species identification.

PLoS ONE, 2019
Variable region analysis of 16S rRNA gene sequences is the most common tool in bacterial taxonomic studies. Although used for distinguishing bacterial species, its use remains limited due to the presence of variable copy numbers with sequence variation ...
Jerald Conrad Ibal, Huy Quang Pham, Chang Eon Park, Jae-Ho Shin   +3 more
doaj   +1 more source

The efficacy of partial 16S rRNA gene sequencing for precise determination of phylogenetic relatedness among Salmonellae

Scientific African, 2021
More than 2,600 Salmonella enterica serovars have been described based on the expression of the somatic and flagellar antigens. For epidemiological tracing, the use of Salmonellae serotyping is inefficient because strains within the same serovar could be
I.M.T. Fadlalla, M.E. Hamid, A.G. ARahim, M.A. Osman   +3 more
doaj   +1 more source

Genetic Environment of 16S rRNA Methylase Gene rmtD [PDF]

Antimicrobial Agents and Chemotherapy, 2008
ABSTRACT The genetic environment of the 16S rRNA methylase gene rmtD was investigated. rmtD was flanked by a novel IS CR motif located downstream of class I integron In 163 in the original Pseudomonas aeruginosa
Doi, Y., Adams-Haduch, J. M., David Paterson   +2 more
openaire   +5 more sources

Defining reference sequences for Nocardia species by similarity and clustering analyses of 16S rRNA gene sequence data. [PDF]

PLoS ONE, 2011
BACKGROUND: The intra- and inter-species genetic diversity of bacteria and the absence of 'reference', or the most representative, sequences of individual species present a significant challenge for sequence-based identification.
Manal Helal, Fanrong Kong, Sharon C A Chen, Michael Bain, Richard Christen, Vitali Sintchenko   +5 more
doaj   +1 more source

Sequence Diversity of Neisseria meningitidis 16S rRNA Genes and Use of 16S rRNA Gene Sequencing as a Molecular Subtyping Tool [PDF]

Journal of Clinical Microbiology, 2002
ABSTRACT We investigated the diversity of the primary sequences of 16S rRNA genes among Neisseria meningitidis strains (Men) and evaluated the use of this approach as a molecular subtyping tool. We aligned and compared a 1,417-bp fragment of the 16S rRNA gene from 264 Men strains of serogroups A, B, C, and
Claudio T, Sacchi, Anne M, Whitney, Michael W, Reeves, Leonard W, Mayer, Tanja, Popovic   +4 more
openaire   +2 more sources

The profiling of microbiota in vaginal swab samples using 16S rRNA gene sequencing and IS-pro analysis

BMC Microbiology, 2021
Background 16S rRNA gene sequencing is currently the most common way of determining the composition of microbiota. This technique has enabled many new discoveries to be made regarding the relevance of microbiota to the health and disease of the host ...
M. Singer, R. Koedooder, M. P. Bos, L. Poort, S. Schoenmakers, P. H. M. Savelkoul, J. S. E. Laven, J. D. de Jonge, S. A. Morré, A. E. Budding   +9 more
doaj   +1 more source

Sequencing of 16S rRNA Gene: A Rapid Tool for Identification of Bacillus anthracis

Emerging Infectious Diseases, 2002
In a bioterrorism event, a tool is needed to rapidly differentiate Bacillus anthracis from other closely related spore-forming Bacillus species. During the recent outbreak of bioterrorism-associated anthrax, we sequenced the 16S rRNA generom these ...
Claudio T. Sacchi, Anne M. Whitney, Leonard W. Mayer, Roger Morey, Arnold Steigerwalt, Ariana Boras, Robin S. Weyant, Tanja Popovic   +7 more
doaj   +1 more source