Results 1 to 10 of about 5,736,241 (346)

Defining Culture Conditions for the Hidden Nitrite-Oxidizing Bacterium Nitrolancea

Frontiers in Microbiology, 2020
Nitrification is a key process for N-removal in engineered and natural environments, but recent findings of novel nitrifying microorganisms with surprising features revealed that our knowledge of this functional guild is still incomplete.
Eva Spieck, Katharina Sass, Sabine Keuter, Sophia Hirschmann, Michael Spohn, Daniela Indenbirken, Linnea F. M. Kop, Sebastian Lücker, Alejandra Giaveno   +8 more
doaj   +1 more source

Methods to improve the accuracy of next-generation sequencing

Frontiers in Bioengineering and Biotechnology, 2023
Next-generation sequencing (NGS) is present in all fields of life science, which has greatly promoted the development of basic research while being gradually applied in clinical diagnosis.
Chu Cheng, Zhongjie Fei, Pengfeng Xiao
semanticscholar   +1 more source

Identification of mutations in laboratory evolved microbes from next-generation sequencing data using breseq

Methods in molecular biology, 2014
Summary Next-generation DNA sequencing (NGS) can be used to reconstruct eco-evolutionary population dynamics and to identify the genetic basis of adaptation in laboratory evolution experiments.
Daniel E. Deatherage, Jeffrey E. Barrick
semanticscholar   +1 more source

The Human Gene Mutation Database: towards a comprehensive repository of inherited mutation data for medical research, genetic diagnosis and next-generation sequencing studies

Human Genetics, 2017
The Human Gene Mutation Database (HGMD®) constitutes a comprehensive collection of published germline mutations in nuclear genes that underlie, or are closely associated with human inherited disease.
Peter D. Stenson, M. Mort, Edward V. Ball, Katy Evans, Matthew Hayden, Sally Heywood, Michelle Hussain, A. D. Phillips, D. Cooper   +8 more
semanticscholar   +1 more source

Next-Generation Sequencing Technologies [PDF]

Cold Spring Harbor Perspectives in Medicine, 2018
Although DNA and RNA sequencing has a history spanning five decades, large-scale massively parallel sequencing, or next-generation sequencing (NGS), has only been commercially available for about 10 years. Nonetheless, the meteoric increase in sequencing throughput with NGS has dramatically changed our understanding of our genome and ourselves ...
McCombie, W Richard, McPherson, John D, Mardis, Elaine R   +2 more
openaire   +4 more sources

The Evolution of Post-Vaccine G8P[4] Group a Rotavirus Strains in Rwanda; Notable Variance at the Neutralization Epitope Sites

Pathogens, 2023
Africa has a high level of genetic diversity of rotavirus strains, which is suggested to be a possible reason contributing to the suboptimal effectiveness of rotavirus vaccines in this region.
Peter N. Mwangi, Robyn-Lee Potgieter, Jeannine Uwimana, Leon Mutesa, Narcisse Muganga, Didier Murenzi, Lisine Tusiyenge, Jason M. Mwenda, Milton T. Mogotsi, Kebareng Rakau, Mathew D. Esona, A. Duncan Steele, Mapaseka L. Seheri, Martin M. Nyaga   +13 more
doaj   +1 more source

Next-Generation Sequencing (NGS) and Third-Generation Sequencing (TGS) for the Diagnosis of Thalassemia

Diagnostics, 2023
Thalassemia is one of the most heterogeneous diseases, with more than a thousand mutation types recorded worldwide. Molecular diagnosis of thalassemia by conventional PCR-based DNA analysis is time- and resource-consuming owing to the phenotype ...
S. Hassan, Rosnah Bahar, M. F. Johan, Ezzeddin Kamil Mohamed Hashim, W. Abdullah, E. Esa, F. S. Abdul Hamid, Z. Zulkafli   +7 more
semanticscholar   +1 more source

Advancements in Next-Generation Sequencing [PDF]

Annual Review of Genomics and Human Genetics, 2016
The term next-generation sequencing is almost a decade old, but it remains the colloquial way to describe highly parallel or high-output sequencing methods that produce data at or beyond the genome scale. Since the introduction of these technologies, the number of applications and methods that leverage the power of genome-scale sequencing has ...
Shawn E, Levy, Richard M, Myers
openaire   +2 more sources

High Detection Frequency of Vaccine-Associated Polioviruses and Non-Polio Enteroviruses in the Stools of Asymptomatic Infants from the Free State Province, South Africa

Microorganisms
Enterovirus (EV) infections are widespread and associated with a range of clinical conditions, from encephalitis to meningitis, gastroenteritis, and acute flaccid paralysis.
Milton T. Mogotsi, Ayodeji E. Ogunbayo, Hester G. O’Neill, Martin M. Nyaga   +3 more
doaj   +1 more source

Next-Generation Sequencing Strategies [PDF]

Cold Spring Harbor Perspectives in Medicine, 2018
More than a decade ago, the term "next-generation" sequencing was coined to describe what was, at the time, revolutionary new methods to sequence RNA and DNA at a faster pace and cheaper cost than could be performed by standard bench-top protocols. Since then, the field of DNA sequencing has evolved at a rapid pace, with new breakthroughs allowing ...
Shawn E, Levy, Braden E, Boone
openaire   +2 more sources