Results 21 to 30 of about 614,915 (309)

High-throughput Quant-iT PicoGreen assay using an automated liquid handling system

BioTechniques, 2019
Workflows in NGS facilities require high-standard practices and high-throughput pipelines to process the large number of samples received in a timely manner. Downstream protocols such as NGS library preparation require accurate estimation of nucleic acid
Kay Anantanawat, Nicola Pitsch, Caroline Fromont, Caroline Janitz   +3 more
doaj   +1 more source

Next-generation sequencing [PDF]

Breast Cancer Research, 2009
Next-generation sequencing (also known as massively parallel sequencing) technologies are revolutionising our ability to characterise cancers at the genomic, transcriptomic and epigenetic levels. Cataloguing all mutations, copy number aberrations and somatic rearrangements in an entire cancer genome at base pair resolution can now be performed in a ...
openaire   +2 more sources

Diagnostic applications of next generation sequencing: working towards quality standards [PDF]

, 2012
Over the past 6 years, next generation sequencing (NGS) has been established as a valuable high-throughput method for research in molecular genetics and has successfully been employed in the identification of rare and common genetic variations. All major
Klein, Hanns-Georg, Biskup, S., Vogl, Ina, Hirv, K., Greif, P.A., Kotschote, S., Gehring, A., Kuhn, M., Stuhrmann, Manfred, Bergmann, C., Eck, Sebastian H., Biskup, Saskia, Metzeler, Klaus H., Greif, Philipp A., Stuhrmann, M., Bolz, Hanno Joern, Benet-Pages, Anna, Hirv, Kaimo, Benet-Pagès, A., Klein, H.G., Vogl, I., Bolz, H.J., Kuhn, Marius, Kotschote, Stefan, Gehring, Andrea, Bergmann, Carsten, Metzeler, K.H., Eck, S.H.   +27 more
core   +1 more source

RADSeq: next-generation population genetics [PDF]

, 2010
Next-generation sequencing technologies are making a substantial impact on many areas of biology, including the analysis of genetic diversity in populations.
Davey, John L, M. L. Blaxter, Blaxter, Mark W, J. W. Davey   +3 more
core   +1 more source

Argonaute3-SF3B3 complex controls pre-mRNA splicing to restrain type 2 immunity

Cell Reports, 2023
Summary: Argonaute (AGO) proteins execute microRNA (miRNA)-mediated gene silencing. However, it is unclear whether all 4 mammalian AGO proteins (AGO1, AGO2, AGO3, and AGO4) are required for miRNA activity. We generate Ago1, Ago3, and Ago4-deficient mice (
Riccardo Guidi, Christopher Wedeles, Daqi Xu, Krzysztof Kolmus, Sarah E. Headland, Grace Teng, Joseph Guillory, Yi Jimmy Zeng, Tommy K. Cheung, Subhra Chaudhuri, Zora Modrusan, Yuxin Liang, Stuart Horswell, Benjamin Haley, Sascha Rutz, Christopher Rose, Yvonne Franke, Donald S. Kirkpatrick, Jason A. Hackney, Mark S. Wilson   +19 more
doaj   +1 more source

Comprehensive Detection of Candidate Pathogens in the Lower Respiratory Tract of Pediatric Patients With Unexpected Cardiopulmonary Deterioration Using Next-Generation Sequencing [PDF]

, 2020
Objectives: Next-generation sequencing has been applied to the investigation of microorganisms in several clinical settings. We investigated the infectious etiologies in respiratory specimens from pediatric patients with unexpected cardiopulmonary ...
Suzuki, Takako, Kazuhiro Horiba, Makoto Yamaguchi, Jun-ichi Kawada, Horiba, Kazuhiro, Toshihiko Okumura, Takanari Ikeyama, Okumura, Toshihiko, Ikeyama, Takanari, Shinji Kawabe, Kawabe, Shinji, Ito, Yoshinori, Yuka Torii, Takeuchi, Suguru, Kawada, Jun-ichi, Sho Wada, Yamaguchi, Makoto, Torii, Yuka, Yoshinori Ito, Suguru Takeuchi, Wada, Sho, Takako Suzuki   +21 more
core   +1 more source

Uncovering the First Atypical DS-1-like G1P[8] Rotavirus Strains That Circulated during Pre-Rotavirus Vaccine Introduction Era in South Africa

Pathogens, 2020
Emergence of DS-1-like G1P[8] group A rotavirus (RVA) strains during post-rotavirus vaccination period has recently been reported in several countries.
Peter N. Mwangi, Milton T. Mogotsi, Sebotsana P. Rasebotsa, Mapaseka L. Seheri, M. Jeffrey Mphahlele, Valantine N. Ndze, Francis E. Dennis, Khuzwayo C. Jere, Martin M. Nyaga   +8 more
doaj   +1 more source

Genomic Analysis of G2P[4] Group A Rotaviruses in Zambia Reveals Positive Selection in Amino Acid Site 7 of Viral Protein 3

Viruses, 2023
The G2P[4] genotype is among the rotavirus strains that circulate commonly in humans. Several countries have reported its immediate upsurge after the introduction of rotavirus vaccination, raising concern about sub-optimal vaccine effectiveness against ...
Peter N. Mwangi, Robyn-Lee Potgieter, Julia Simwaka, Evans M. Mpabalwani, Jason M. Mwenda, Milton T. Mogotsi, Nonkululeko Magagula, Mathew D. Esona, A. Duncan Steele, Mapaseka L. Seheri, Martin M. Nyaga   +10 more
doaj   +1 more source

Next-generation sequencing in dermatology

Frontiers in Medicine, 2023
Over the past decade, Next-Generation Sequencing (NGS) has advanced our understanding, diagnosis, and management of several areas within dermatology. NGS has emerged as a powerful tool for diagnosing genetic diseases of the skin, improving upon traditional PCR-based techniques limited by significant genetic heterogeneity associated with these disorders.
Andrew D. King, Hany Deirawan, Paytra A. Klein, Bahar Dasgeb, Catherine I. Dumur, Darius R. Mehregan   +5 more
openaire   +3 more sources

A genome-wide survey of mutations in the Jurkat cell line

BMC Genomics, 2018
Background The Jurkat cell line has an extensive history as a model of T cell signaling. But at the turn of the 21st century, some expression irregularities were observed, raising doubts about how closely the cell line paralleled normal human T cells ...
Louis Gioia, Azeem Siddique, Steven R. Head, Daniel R. Salomon, Andrew I. Su   +4 more
doaj   +1 more source