Results 21 to 30 of about 5,736,241 (346)

Metagenomic Analysis of Respiratory RNA Virome of Children with and without Severe Acute Respiratory Infection from the Free State, South Africa during COVID-19 Pandemic Reveals Higher Diversity and Abundance in Summer Compared with Winter Period

Viruses, 2022
Viral respiratory infections contribute to significant morbidity and mortality in children. Currently, there are limited reports on the composition and abundance of the normal commensal respiratory virome in comparison to those in severe acute ...
Ayodeji E. Ogunbayo, Milton T. Mogotsi, Hlengiwe Sondlane, Kelebogile R. Nkwadipo, Saheed Sabiu, Martin M. Nyaga   +5 more
doaj   +1 more source

ResFinder – an open online resource for identification of antimicrobial resistance genes in next-generation sequencing data and prediction of phenotypes from genotypes

Microbial Genomics, 2022
Antimicrobial resistance (AMR) is one of the most important health threats globally. The ability to accurately identify resistant bacterial isolates and the individual antimicrobial resistance genes (ARGs) is essential for understanding the evolution and
A. F. Florensa, R. Kaas, P. T. Clausen, D. Aytan-Aktug, F. Aarestrup   +4 more
semanticscholar   +1 more source

Next-generation sequencing demands next-generation phenotyping [PDF]

Human Mutation, 2012
Next-generation sequencing (NGS) is the most powerful diagnostic tool since the roentgenogram. NGS will facilitate diagnosis on a massive scale, allowing interrogation of all genes in a single assay. It has been suggested that NGS will decrease the need for phenotyping in general and medical geneticists in particular. We argue that NGS will shift focus
Hennekam, Raoul C. M., Biesecker, Leslie G.   +1 more
openaire   +3 more sources

Next generation sequencing in cytology [PDF]

Cytopathology, 2021
AbstractThe application of next generation sequencing (NGS) technology to cytological samples has significantly modified molecular cytopathology practice. Cytological samples represent a valid source of high‐quality DNA for NGS analysis, especially for predicting patients' response to targeted treatments and for refining the risk of malignancy in ...
Pisapia, Pasquale, Pepe, Francesco, Sgariglia, Roberta, Nacchio, Mariantonia, Russo, Gianluca, Conticelli, Floriana, Girolami, Ilaria, Eccher, Albino, Bellevicine, Claudio, Vigliar, Elena, Malapelle, Umberto, Troncone, Giancarlo   +11 more
openaire   +3 more sources

In silico prediction of the enzymes involved in the degradation of the herbicide molinate by Gulosibacter molinativorax ON4T

Scientific Reports, 2022
Gulosibacter molinativorax ON4T is the only known organism to produce molinate hydrolase (MolA), which catalyses the breakdown of the thiocarbamate herbicide into azepane-1-carboxylic acid (ACA) and ethanethiol.
A. R. Lopes, E. Bunin, A. T. Viana, H. Froufe, A. Muñoz-Merida, D. Pinho, J. Figueiredo, C. Barroso, I. Vaz-Moreira, X. Bellanger, C. Egas, O. C. Nunes   +11 more
doaj   +1 more source

High-throughput Quant-iT PicoGreen assay using an automated liquid handling system

BioTechniques, 2019
Workflows in NGS facilities require high-standard practices and high-throughput pipelines to process the large number of samples received in a timely manner. Downstream protocols such as NGS library preparation require accurate estimation of nucleic acid
Kay Anantanawat, Nicola Pitsch, Caroline Fromont, Caroline Janitz   +3 more
doaj   +1 more source

Transcriptomic studies reveal a key metabolic pathway contributing to a well-maintained photosynthetic system under drought stress in foxtail millet (Setaria italica L.) [PDF]

PeerJ, 2018
Drought stress is one of the most important abiotic factors limiting crop productivity. A better understanding of the effects of drought on millet (Setaria italica L.) production, a model crop for studying drought tolerance, and the underlying molecular ...
Weiping Shi, Jingye Cheng, Xiaojie Wen, Jixiang Wang, Guanyan Shi, Jiayan Yao, Liyuan Hou, Qian Sun, Peng Xiang, Xiangyang Yuan, Shuqi Dong, Pingyi Guo, Jie Guo   +12 more
doaj   +2 more sources

CD-HIT: accelerated for clustering the next-generation sequencing data

Bioinform., 2012
Summary: CD-HIT is a widely used program for clustering biological sequences to reduce sequence redundancy and improve the performance of other sequence analyses.
Limin Fu, Beifang Niu, Zhengwei Zhu, Sitao Wu, Weizhong Li   +4 more
semanticscholar   +1 more source

Argonaute3-SF3B3 complex controls pre-mRNA splicing to restrain type 2 immunity

Cell Reports, 2023
Summary: Argonaute (AGO) proteins execute microRNA (miRNA)-mediated gene silencing. However, it is unclear whether all 4 mammalian AGO proteins (AGO1, AGO2, AGO3, and AGO4) are required for miRNA activity. We generate Ago1, Ago3, and Ago4-deficient mice (
Riccardo Guidi, Christopher Wedeles, Daqi Xu, Krzysztof Kolmus, Sarah E. Headland, Grace Teng, Joseph Guillory, Yi Jimmy Zeng, Tommy K. Cheung, Subhra Chaudhuri, Zora Modrusan, Yuxin Liang, Stuart Horswell, Benjamin Haley, Sascha Rutz, Christopher Rose, Yvonne Franke, Donald S. Kirkpatrick, Jason A. Hackney, Mark S. Wilson   +19 more
doaj   +1 more source

deepTools2: a next generation web server for deep-sequencing data analysis

Nucleic Acids Res., 2016
We present an update to our Galaxy-based web server for processing and visualizing deeply sequenced data. Its core tool set, deepTools, allows users to perform complete bioinformatic workflows ranging from quality controls and normalizations of aligned ...
Fidel Ramírez, D. Ryan, Björn A. Grüning, V. Bhardwaj, Fabian Kilpert, Andreas S. Richter, Steffen Heyne, F. Dündar, T. Manke   +8 more
semanticscholar   +1 more source